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A5FGV5

- DEF_FLAJ1

UniProt

A5FGV5 - DEF_FLAJ1

Protein

Peptide deformylase

Gene

def

Organism
Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / UW101) (Cytophaga johnsonae)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 1 (12 Jun 2007)
      Previous versions | rss
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    Functioni

    Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions.UniRule annotation

    Catalytic activityi

    Formyl-L-methionyl peptide + H2O = formate + methionyl peptide.UniRule annotation

    Cofactori

    Binds 1 Fe2+ ion.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi105 – 1051IronUniRule annotation
    Metal bindingi147 – 1471IronUniRule annotation
    Active sitei148 – 1481UniRule annotation
    Metal bindingi151 – 1511IronUniRule annotation

    GO - Molecular functioni

    1. iron ion binding Source: InterPro
    2. peptide deformylase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. translation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciFJOH376686:GIXN-2589-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Peptide deformylaseUniRule annotation (EC:3.5.1.88UniRule annotation)
    Short name:
    PDFUniRule annotation
    Alternative name(s):
    Polypeptide deformylaseUniRule annotation
    Gene namesi
    Name:defUniRule annotation
    Ordered Locus Names:Fjoh_2532
    OrganismiFlavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / UW101) (Cytophaga johnsonae)
    Taxonomic identifieri376686 [NCBI]
    Taxonomic lineageiBacteriaBacteroidetesFlavobacteriiaFlavobacterialesFlavobacteriaceaeFlavobacterium
    ProteomesiUP000006694: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 196196Peptide deformylasePRO_1000076944Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi376686.Fjoh_2532.

    Structurei

    3D structure databases

    ProteinModelPortaliA5FGV5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the polypeptide deformylase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0242.
    HOGENOMiHOG000243509.
    KOiK01462.
    OMAiPNQYAEV.
    OrthoDBiEOG664CMF.

    Family and domain databases

    Gene3Di3.90.45.10. 1 hit.
    HAMAPiMF_00163. Pep_deformylase.
    InterProiIPR000181. Fmet_deformylase.
    IPR023635. Peptide_deformylase.
    [Graphical view]
    PANTHERiPTHR10458. PTHR10458. 1 hit.
    PfamiPF01327. Pep_deformylase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF004749. Pep_def. 1 hit.
    PRINTSiPR01576. PDEFORMYLASE.
    SUPFAMiSSF56420. SSF56420. 1 hit.
    TIGRFAMsiTIGR00079. pept_deformyl. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A5FGV5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MILPIVGYGD PVLRKVGTAI TPDYPNLKET IANMYETMYN AYGVGLAAPQ    50
    VGLPIRIFVI DTTPFSDDED LPADEQKDLK GFKRTFINAK IVKEEGEEWS 100
    FNEGCLSIPD VREDVYRKPT VTIEYCEEDF VMKTEVFDGL IARVIQHEYD 150
    HIEGVLFTDK ISSLKKRLIQ KKLKNITEGK TFQEYRMKFA AAKKGR 196
    Length:196
    Mass (Da):22,410
    Last modified:June 12, 2007 - v1
    Checksum:iDDB295FFA9FACCEC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000685 Genomic DNA. Translation: ABQ05559.1.
    RefSeqiYP_001194878.1. NC_009441.1.

    Genome annotation databases

    EnsemblBacteriaiABQ05559; ABQ05559; Fjoh_2532.
    GeneIDi5091986.
    KEGGifjo:Fjoh_2532.
    PATRICi21899503. VBIFlaJoh53613_2603.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000685 Genomic DNA. Translation: ABQ05559.1 .
    RefSeqi YP_001194878.1. NC_009441.1.

    3D structure databases

    ProteinModelPortali A5FGV5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 376686.Fjoh_2532.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABQ05559 ; ABQ05559 ; Fjoh_2532 .
    GeneIDi 5091986.
    KEGGi fjo:Fjoh_2532.
    PATRICi 21899503. VBIFlaJoh53613_2603.

    Phylogenomic databases

    eggNOGi COG0242.
    HOGENOMi HOG000243509.
    KOi K01462.
    OMAi PNQYAEV.
    OrthoDBi EOG664CMF.

    Enzyme and pathway databases

    BioCyci FJOH376686:GIXN-2589-MONOMER.

    Family and domain databases

    Gene3Di 3.90.45.10. 1 hit.
    HAMAPi MF_00163. Pep_deformylase.
    InterProi IPR000181. Fmet_deformylase.
    IPR023635. Peptide_deformylase.
    [Graphical view ]
    PANTHERi PTHR10458. PTHR10458. 1 hit.
    Pfami PF01327. Pep_deformylase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF004749. Pep_def. 1 hit.
    PRINTSi PR01576. PDEFORMYLASE.
    SUPFAMi SSF56420. SSF56420. 1 hit.
    TIGRFAMsi TIGR00079. pept_deformyl. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis."
      McBride M.J., Xie G., Martens E.C., Lapidus A., Henrissat B., Rhodes R.G., Goltsman E., Wang W., Xu J., Hunnicutt D.W., Staroscik A.M., Hoover T.R., Cheng Y.Q., Stein J.L.
      Appl. Environ. Microbiol. 75:6864-6875(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 17061 / DSM 2064 / UW101.

    Entry informationi

    Entry nameiDEF_FLAJ1
    AccessioniPrimary (citable) accession number: A5FGV5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: June 12, 2007
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3