A5FEX8 (A5FEX8_FLAJ1) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase 2 HAMAP MF_00012
Short name=DAD 2 HAMAP MF_00012
EC=4.2.1.9 HAMAP MF_00012

Gene names

Name:	ilvD2 HAMAP MF_00012
Ordered Locus Names:	Fjoh_3218

Organism

Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / UW101) (Cytophaga johnsonae) [Complete proteome] [HAMAP]

Taxonomic identifier

376686 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Flavobacteriia › Flavobacteriales › Flavobacteriaceae › Flavobacterium

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster By similarity. HAMAP MF_00012
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family. HAMAP MF_00012

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis HAMAP MF_00012
Ligand	4Fe-4S HAMAP MF_00012 Iron Iron-sulfur Metal-binding
Molecular function	Lyase HAMAP MF_00012 EMBL ABQ06235.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	119	1	Iron-sulfur (4Fe-4S) By similarity HAMAP MF_00012
	Metal binding	192	1	Iron-sulfur (4Fe-4S) By similarity HAMAP MF_00012

Sequences

	Sequence		Length	Mass (Da)
	A5FEX8 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: E5B52E5A699DCF98	FASTA	562	59,812
	10 20 30 40 50 60 MRSDEVKKGV QRTPHRSLLR ATGLKNEDFG KPFIGVANSF IEIIPGHFFL NKVSEIIKEE 70 80 90 100 110 120 IRANGCVPFE FNTIGIDDGI AMGHDGMLYS LPSREIIANS IESVMNAHKL DAMIAIPNCD 130 140 150 160 170 180 KIVPGMIMGA LRVDVPTIFV SGGPMSKGYT KNGTPIDLAT AFEAVGKHEA GSITDEELYD 190 200 210 220 230 240 IECNACPSGG SCSGMFTANS MNTLMEAMGI ALPGNGTILA QTPEREQLYR KAARRICAIA 250 260 270 280 290 300 KDQHDIEKFK LKNILNENAV RNAFAVDMAM GGSSNTVLHM LAIANEANVD FKLKDINTIS 310 320 330 340 350 360 GNVSHIAKIS PSLSTVHMED INRAGGVNAV MKEMNKRGSG VLIDNLTISG ETLLEKIDNA 370 380 390 400 410 420 EIKDTTIIHT IDNPYSSVGG LAVLYGNLAE EGAVIKTAGL TGARVFTGKA VCFDGQAEAI 430 440 450 460 470 480 AGIMMGKVKA GNVVVIRYEG PKGGPGMQEM LAPTSLIMGM GLGSSVALIT DGRFSGATRG 490 500 510 520 530 540 ASIGHVSPEA AEGGMIGLLK DGDEIHIDVD QYILSVNLSD EEIKTRKAAF QPIKKVLNSK 550 560 WLGQYRALVT NASNGAILKT DL

References

[1]

"Complete sequence of Flavobacterium johnsoniae UW101." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Goltsman E., Singan V., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Xie G., McBride M., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17061 / DSM 2064 / UW101.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000685 Genomic DNA. Translation: ABQ06235.1.
RefSeq	YP_001195554.1. NC_009441.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A5FEX8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5093380.
GenomeReviews	Gene locus Fjoh_3218 in contig CP000685_GR.
KEGG	fjo:Fjoh_3218.
PATRIC	21900985. VBIFlaJoh53613_3335.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0129.
HOGENOM	HBG671001.
OMA	SQDCRWP.
ProtClustDB	PRK00911.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A5FEX8_FLAJ1

Accession

Primary (citable) accession number: A5FEX8

Entry history

Integrated into UniProtKB/TrEMBL:	June 12, 2007
Last sequence update:	June 12, 2007
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)