A5FD11 (KATG_FLAJ1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	Fjoh_3897

Organism

Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / UW101) (Cytophaga johnsonae) [Complete proteome] [HAMAP]

Taxonomic identifier

376686 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Flavobacteriia › Flavobacteriales › Flavobacteriaceae › Flavobacterium

Protein attributes

Sequence length	757 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 757	757	Catalase-peroxidase HAMAP MF_01961		PRO_0000354787
Sites
	Active site	101	1	Proton acceptor By similarity
	Metal binding	288	1	Iron (heme axial ligand) By similarity
	Site	97	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	100 ↔ 247		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-273) By similarity
	Cross-link	247 ↔ 273		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-100) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5FD11 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 9BC9972154BAE01B	FASTA	757	83,445
	10 20 30 40 50 60 MENQSNDISK CPFHNGSMDN QAASGTKNND WWPKQLKVNI LRQNSSLSNP LSKDFDYAEA 70 80 90 100 110 120 FKTLDLEAVK KDLHVLMTDS QDWWPADFGH YGGLFIRMAW HSAGTYRVHD GRGGAGAGQQ 130 140 150 160 170 180 RFAPLNSWPD NVSLDKARRL LWPIKQKYGQ KISWADLMIL TGNVALESMG FKTFGFAGGR 190 200 210 220 230 240 ADVWEPDESV YWGSETTWLG GDERYNNGSD GVPKDHGVVS ADDDADGKVH SRNLEKPLAA 250 260 270 280 290 300 VQMGLIYVNP EGPDGNPDPI LAAKDIRDTF GRMAMNDEET VALIAGGHTF GKTHGAASSD 310 320 330 340 350 360 HVDKEPEAAG LELQGFGWKN SFGSGKGADA ITSGLEVTWT KTPTQWSNNF FENLFAFEWE 370 380 390 400 410 420 LSKSPAGAHQ WVAKNAEAII PDAFDSTKKH LPTMLTTDLS LRLDPEYEKI SRRFLENPDQ 430 440 450 460 470 480 FADAFSRAWF KLTHRDMGPR ARYLGPDVPQ EVLLWQDPIP EVNHKLIDEN DIKQLKEKIL 490 500 510 520 530 540 NSGLSISQLV AAAWASASTF RGSDKRGGAN GARVRLAPQK DWEVNNPAKL AQVLSKLETI 550 560 570 580 590 600 QTEFNASQND GKKVSLADLI VLAGSAGVEK AAKDAGSSVT VSFNPGRMDA SAEETDVESF 610 620 630 640 650 660 GYLEPKADGF RNYRKTKSAV STEELLIDKA NLLTLTAPEL TVLLGGLRVL DINADGSKNG 670 680 690 700 710 720 VFTHRPGQLT NDFFVNLLDM NTQWQAVSND KELYAGNDRS TGQPKWIATR ADLVFGSNSE 730 740 750 LRAVAEVYAS TDANEKFVND FIKAWTKVMN LDRFDLA

References

[1]

"Complete sequence of Flavobacterium johnsoniae UW101." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Goltsman E., Singan V., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Xie G., McBride M., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17061 / DSM 2064 / UW101.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000685 Genomic DNA. Translation: ABQ06908.1.
RefSeq	YP_001196227.1. NC_009441.1.
3D structure databases
ProteinModelPortal	A5FD11.
SMR	A5FD11. Positions 24-757.
ModBase	Search...
Protein-protein interaction databases
STRING	A5FD11.
Protein family/group databases
PeroxiBase	3617. FjCP01_UW101.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5090128.
GenomeReviews	Gene locus Fjoh_3897 in contig CP000685_GR.
KEGG	fjo:Fjoh_3897.
PATRIC	21902389. VBIFlaJoh53613_4037.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0376.
HOGENOM	HBG285610.
OMA	KRHAPSM.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	FJOH376686:FJOH_3897-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_FLAJ1

Accession

Primary (citable) accession number: A5FD11

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	June 12, 2007
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program