A5FBA3 (KDSB_FLAJ1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-deoxy-manno-octulosonate cytidylyltransferase
EC=2.7.7.38

Alternative name(s):
CMP-2-keto-3-deoxyoctulosonic acid synthase
Short name=CKS
Short name=CMP-KDO synthase

Gene names

Name:	kdsB
Ordered Locus Names:	Fjoh_4513

Organism

Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / UW101) (Cytophaga johnsonae) [Complete proteome] [HAMAP]

Taxonomic identifier

376686 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Flavobacteriia › Flavobacteriales › Flavobacteriaceae › Flavobacterium

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria By similarity. HAMAP MF_00057
Catalytic activity	CTP + 3-deoxy-D-manno-octulosonate = diphosphate + CMP-3-deoxy-D-manno-octulosonate. HAMAP MF_00057
Pathway	Nucleotide-sugar biosynthesis; CMP-3-deoxy-D-manno-octulosonate biosynthesis; CMP-3-deoxy-D-manno-octulosonate from 3-deoxy-D-manno-octulosonate and CTP: step 1/1. HAMAP MF_00057 Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. HAMAP MF_00057
Subcellular location	Cytoplasm Potential HAMAP MF_00057.
Sequence similarities	Belongs to the KdsB family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cytoplasm
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-deoxy-manno-octulosonate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 244	244	3-deoxy-manno-octulosonate cytidylyltransferase HAMAP MF_00057		PRO_1000116889

Sequences

	Sequence		Length	Mass (Da)
	A5FBA3 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 7D3598843568BF44	FASTA	244	27,468
	10 20 30 40 50 60 MKIIAVIPAR YASTRFPAKL MQDLGGKTVI LRTYEAAVST KLFDDVFVVT DSDLIFDEIV 70 80 90 100 110 120 SNGGKAIMSI KEHESGSDRI AEAVANLDVD IVVNVQGDEP FTEAGPLEQV LSVFKNDPDK 130 140 150 160 170 180 KIDLASLMRE ITNEDEINNP NNVKVVVDQS QFALYFSRSV IPYPREKDAG VRYFQHIGIY 190 200 210 220 230 240 AFRKQALLDF YSLPMKSLEA SEKLEQLRYL EFGKRIKMVE TTHVGIGIDT AEDLERARAM LRDI

References

[1]

"Complete sequence of Flavobacterium johnsoniae UW101." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Goltsman E., Singan V., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Xie G., McBride M., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17061 / DSM 2064 / UW101.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000685 Genomic DNA. Translation: ABQ07512.1.
RefSeq	YP_001196831.1. NC_009441.1.
3D structure databases
ProteinModelPortal	A5FBA3.
ModBase	Search...
Protein-protein interaction databases
STRING	A5FBA3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5092586.
GenomeReviews	Gene locus Fjoh_4513 in contig CP000685_GR.
KEGG	fjo:Fjoh_4513.
PATRIC	21903661. VBIFlaJoh53613_4668.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1212.
HOGENOM	HBG637773.
OMA	INVQADE.
ProtClustDB	CLSK851559.
Enzyme and pathway databases
BioCyc	FJOH376686:FJOH_4513-MONOMER.
Family and domain databases
HAMAP	MF_00057. CMP-KDO_synth. [Tree]
InterPro	IPR003329. Cytidylyl_trans. IPR004528. KdsB. [Graphical view]
KO	K00979.
PANTHER	PTHR21485:SF4. PTHR21485:SF4. 1 hit.
Pfam	PF02348. CTP_transf_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00466. KdsB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDSB_FLAJ1

Accession

Primary (citable) accession number: A5FBA3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	June 12, 2007
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program