A5FB49 (HUTI_FLAJ1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Imidazolonepropionase
EC=3.5.2.7

Alternative name(s):
Imidazolone-5-propionate hydrolase

Gene names

Name:	hutI
Ordered Locus Names:	Fjoh_4570

Organism

Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / UW101) (Cytophaga johnsonae) [Complete proteome] [HAMAP]

Taxonomic identifier

376686 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Flavobacteriia › Flavobacteriales › Flavobacteriaceae › Flavobacterium

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+. HAMAP MF_00372
Cofactor	Binds 1 zinc or iron ion per subunit By similarity. HAMAP MF_00372
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 3/3. HAMAP MF_00372
Subcellular location	Cytoplasm Potential HAMAP MF_00372.
Sequence similarities	Belongs to the HutI family.

Ontologies

Keywords
Biological process	Histidine metabolism
Cellular component	Cytoplasm
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	histidine catabolic process to glutamate and formamide Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	imidazolonepropionase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 411	411	Imidazolonepropionase HAMAP MF_00372		PRO_1000079822
Sites
	Metal binding	78	1	Zinc or iron By similarity
	Metal binding	80	1	Zinc or iron By similarity
	Metal binding	248	1	Zinc or iron By similarity
	Metal binding	322	1	Zinc or iron By similarity
	Binding site	87	1	Substrate By similarity
	Binding site	100	1	Substrate By similarity
	Binding site	150	1	Substrate By similarity
	Binding site	183	1	Substrate By similarity
	Binding site	251	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5FB49 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: A4542F936046042F	FASTA	411	45,076
	10 20 30 40 50 60 MITLIKNIQE LLQVRQTSIS KVSGAEMAEL PTIKNAFLVI KDDLIADFGS MENLPEIKAD 70 80 90 100 110 120 SIINASGRVV LPAWCDSHTH IVYAGNREQE FVDRINGFTY EEIANRGGGI LNSAKKLNET 130 140 150 160 170 180 SEEEIYEQSK IRLEEVMRLG TGAVEIKSGY GLTVEGELKM LRVIKKLAEN YPISIKATFL 190 200 210 220 230 240 GAHAFPTHYK ENKAGYIDEI ITKMLPEIAQ NKLADYVDVF CESGYFSVEE TEKIMQAGID 250 260 270 280 290 300 FGLKPKIHVN QFNSIGGIQS GVKFNALSVD HLEIMNPEDI EALKGTETMP VALPSCSYFL 310 320 330 340 350 360 SIPYTPAREM IKAGLPLALA TDFNPGSTPS GNMNFVVATA CIKMKMTPEE AINAATINGA 370 380 390 400 410 YAMGLSETHG SITKGKKANL IITKPISSYY QIPYAFGSNL IEDVLIDGQI I

References

[1]

"Complete sequence of Flavobacterium johnsoniae UW101." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Goltsman E., Singan V., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Xie G., McBride M., Richardson P. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17061 / DSM 2064 / UW101.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000685 Genomic DNA. Translation: ABQ07569.1.
RefSeq	YP_001196888.1. NC_009441.1.
3D structure databases
ProteinModelPortal	A5FB49.
ModBase	Search...
Protein-protein interaction databases
STRING	A5FB49.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5092881.
GenomeReviews	Gene locus Fjoh_4570 in contig CP000685_GR.
KEGG	fjo:Fjoh_4570.
PATRIC	21903777. VBIFlaJoh53613_4726.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1228.
HOGENOM	HBG686142.
OMA	MNMACTL.
ProtClustDB	PRK09356.
Enzyme and pathway databases
BioCyc	FJOH376686:FJOH_4570-MONOMER.
Family and domain databases
HAMAP	MF_00372. HutI. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR005920. HutI. IPR011059. Metal-dep_hydrolase_composite. [Graphical view]
KO	K01468.
PANTHER	PTHR22642. PTHR22642. 1 hit.
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
SUPFAM	SSF51338. Metalo_hydrolase. 1 hit.
TIGRFAMs	TIGR01224. HutI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HUTI_FLAJ1

Accession

Primary (citable) accession number: A5FB49

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	June 12, 2007
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program