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A5F8Z3

- SYI_VIBC3

UniProt

A5F8Z3 - SYI_VIBC3

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei567 – 5671Aminoacyl-adenylateUniRule annotation
Binding sitei611 – 6111ATPUniRule annotation
Metal bindingi912 – 9121ZincUniRule annotation
Metal bindingi915 – 9151ZincUniRule annotation
Metal bindingi932 – 9321ZincUniRule annotation
Metal bindingi935 – 9351ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:VC0395_A0214, VC395_0699
OrganismiVibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)
Taxonomic identifieri345073 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000000249: Chromosome 2, UP000001630: Chromosome I

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 949949Isoleucine--tRNA ligasePRO_1000073712Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi345073.VC0395_A0214.

Structurei

3D structure databases

ProteinModelPortaliA5F8Z3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi608 – 6125"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5F8Z3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSEYKDTLNL PETGFPMRGD LAKREPEMLQ RWYQEDLYGA IRQAKKGKKS
60 70 80 90 100
FVLHDGPPYA NGDIHIGHAL NKILKDVIIK SKTLSGFDAP YIPGWDCHGL
110 120 130 140 150
PIELMVEKKV GKPGQKVTAA EFREKCREYA AGQVEGQKES FKRLGILGEW
160 170 180 190 200
DKPYRTMDFV TEANIIRALG KIADNGHLLK GFKPVHWCTD CGSALAEAEV
210 220 230 240 250
EYKNKVSPSI DVRFKAADEA AVLAKFGLAA GHEGKGDVSI VIWTTTPWTL
260 270 280 290 300
PANRAVCLRA DLEYVLIQVE GEQPERIIVA SELAKSVMDR AGIEHFHNLG
310 320 330 340 350
FATGADLELV QFQHPFYSFT VPAILGDHVT TDSGTGVVHT APGHGQEDFA
360 370 380 390 400
VGQQYGLEVA NPVGSNGVYL PDTELFAGQH VFKANDSVLE VLKEKGALLH
410 420 430 440 450
HHAYEHSYPH CWRHKTPIIF RATPQWFVSM EQAGLREQAL TAIKGVHWMP
460 470 480 490 500
DWGQSRIEGM VAGRPEWCIS RQRTWGVPIA LFVHKETAEL HPNSADLIEK
510 520 530 540 550
VAQLVEQKGI QAWWDLDTAE LLGAEDAANY EKVLDTLDVW FDSGVTHSAV
560 570 580 590 600
VDARQEFNGA EADMYLEGSD QHRGWFQSSL ISSVAMKSKA PYKEVLTHGF
610 620 630 640 650
VVDGQGRKMS KSIGNVVAPQ DVTNKLGADI LRLWVASTDY TGEVAVSDEI
660 670 680 690 700
LKRSADAYRR IRNTARFFLA NLNGFNPTTD IIPVEDMVAL DRWAVGRALA
710 720 730 740 750
AQQEIIQAYQ DYNLHAVVQR LMNFCSIEMG SFYLDVIKDR QYTAKRGGHA
760 770 780 790 800
QRSCQTALFF IVEALVRWMA PIMSFTADEI WNAMPAQQAD GSARDKFVFT
810 820 830 840 850
TEWFDGLFGL AEGEELNNAF WNDIQKVRGS VNKLLENARN EKLIGGSLQA
860 870 880 890 900
ELVLFADDAL ASKLAKLGDE LRFVLLTSKA VVKPLAEKSE AAQATDIDGL
910 920 930 940
FVQVNKTEAE KCDRCWHHTP DVGTIAGHTT ICGRCVSNVE GEGEVRKFA
Length:949
Mass (Da):105,413
Last modified:June 12, 2007 - v1
Checksum:i5B4212359E2AD71A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000627 Genomic DNA. Translation: ABQ21661.1.
CP001235 Genomic DNA. Translation: ACP08717.1.
RefSeqiYP_001216177.1. NC_009457.1.
YP_002818953.1. NC_012582.1.

Genome annotation databases

EnsemblBacteriaiABQ21661; ABQ21661; VC0395_A0214.
ACP08717; ACP08717; VC395_0699.
GeneIDi5137625.
7774718.
KEGGivco:VC0395_A0214.
vcr:VC395_0699.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000627 Genomic DNA. Translation: ABQ21661.1 .
CP001235 Genomic DNA. Translation: ACP08717.1 .
RefSeqi YP_001216177.1. NC_009457.1.
YP_002818953.1. NC_012582.1.

3D structure databases

ProteinModelPortali A5F8Z3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 345073.VC0395_A0214.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABQ21661 ; ABQ21661 ; VC0395_A0214 .
ACP08717 ; ACP08717 ; VC395_0699 .
GeneIDi 5137625.
7774718.
KEGGi vco:VC0395_A0214.
vcr:VC395_0699.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Heidelberg J.
    Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39541 / Classical Ogawa 395 / O395.
  2. "A recalibrated molecular clock and independent origins for the cholera pandemic clones."
    Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J., Wang W., Wang J., Qian W., Li D., Wang L.
    PLoS ONE 3:E4053-E4053(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39541 / Classical Ogawa 395 / O395.

Entry informationi

Entry nameiSYI_VIBC3
AccessioniPrimary (citable) accession number: A5F8Z3
Secondary accession number(s): C3LXZ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: June 12, 2007
Last modified: October 29, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3