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A5F8Z3

- SYI_VIBC3

UniProt

A5F8Z3 - SYI_VIBC3

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Protein

Isoleucine--tRNA ligase

Gene
ileS, VC0395_A0214, VC395_0699
Organism
Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei567 – 5671Aminoacyl-adenylate By similarity
Binding sitei611 – 6111ATP By similarity
Metal bindingi912 – 9121Zinc By similarity
Metal bindingi915 – 9151Zinc By similarity
Metal bindingi932 – 9321Zinc By similarity
Metal bindingi935 – 9351Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:VC0395_A0214, VC395_0699
OrganismiVibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)
Taxonomic identifieri345073 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000000249: Chromosome 2, UP000001630: Chromosome I

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 949949Isoleucine--tRNA ligaseUniRule annotationPRO_1000073712Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi345073.VC0395_A0214.

Structurei

3D structure databases

ProteinModelPortaliA5F8Z3.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotationAdd
BLAST
Motifi608 – 6125"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5F8Z3-1 [UniParc]FASTAAdd to Basket

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MSEYKDTLNL PETGFPMRGD LAKREPEMLQ RWYQEDLYGA IRQAKKGKKS    50
FVLHDGPPYA NGDIHIGHAL NKILKDVIIK SKTLSGFDAP YIPGWDCHGL 100
PIELMVEKKV GKPGQKVTAA EFREKCREYA AGQVEGQKES FKRLGILGEW 150
DKPYRTMDFV TEANIIRALG KIADNGHLLK GFKPVHWCTD CGSALAEAEV 200
EYKNKVSPSI DVRFKAADEA AVLAKFGLAA GHEGKGDVSI VIWTTTPWTL 250
PANRAVCLRA DLEYVLIQVE GEQPERIIVA SELAKSVMDR AGIEHFHNLG 300
FATGADLELV QFQHPFYSFT VPAILGDHVT TDSGTGVVHT APGHGQEDFA 350
VGQQYGLEVA NPVGSNGVYL PDTELFAGQH VFKANDSVLE VLKEKGALLH 400
HHAYEHSYPH CWRHKTPIIF RATPQWFVSM EQAGLREQAL TAIKGVHWMP 450
DWGQSRIEGM VAGRPEWCIS RQRTWGVPIA LFVHKETAEL HPNSADLIEK 500
VAQLVEQKGI QAWWDLDTAE LLGAEDAANY EKVLDTLDVW FDSGVTHSAV 550
VDARQEFNGA EADMYLEGSD QHRGWFQSSL ISSVAMKSKA PYKEVLTHGF 600
VVDGQGRKMS KSIGNVVAPQ DVTNKLGADI LRLWVASTDY TGEVAVSDEI 650
LKRSADAYRR IRNTARFFLA NLNGFNPTTD IIPVEDMVAL DRWAVGRALA 700
AQQEIIQAYQ DYNLHAVVQR LMNFCSIEMG SFYLDVIKDR QYTAKRGGHA 750
QRSCQTALFF IVEALVRWMA PIMSFTADEI WNAMPAQQAD GSARDKFVFT 800
TEWFDGLFGL AEGEELNNAF WNDIQKVRGS VNKLLENARN EKLIGGSLQA 850
ELVLFADDAL ASKLAKLGDE LRFVLLTSKA VVKPLAEKSE AAQATDIDGL 900
FVQVNKTEAE KCDRCWHHTP DVGTIAGHTT ICGRCVSNVE GEGEVRKFA 949
Length:949
Mass (Da):105,413
Last modified:June 12, 2007 - v1
Checksum:i5B4212359E2AD71A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000627 Genomic DNA. Translation: ABQ21661.1.
CP001235 Genomic DNA. Translation: ACP08717.1.
RefSeqiYP_001216177.1. NC_009457.1.
YP_002818953.1. NC_012582.1.

Genome annotation databases

EnsemblBacteriaiABQ21661; ABQ21661; VC0395_A0214.
ACP08717; ACP08717; VC395_0699.
GeneIDi5137625.
7774718.
KEGGivco:VC0395_A0214.
vcr:VC395_0699.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000627 Genomic DNA. Translation: ABQ21661.1 .
CP001235 Genomic DNA. Translation: ACP08717.1 .
RefSeqi YP_001216177.1. NC_009457.1.
YP_002818953.1. NC_012582.1.

3D structure databases

ProteinModelPortali A5F8Z3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 345073.VC0395_A0214.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABQ21661 ; ABQ21661 ; VC0395_A0214 .
ACP08717 ; ACP08717 ; VC395_0699 .
GeneIDi 5137625.
7774718.
KEGGi vco:VC0395_A0214.
vcr:VC395_0699.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Heidelberg J.
    Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39541 / Classical Ogawa 395 / O395.
  2. "A recalibrated molecular clock and independent origins for the cholera pandemic clones."
    Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J., Wang W., Wang J., Qian W., Li D., Wang L.
    PLoS ONE 3:E4053-E4053(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39541 / Classical Ogawa 395 / O395.

Entry informationi

Entry nameiSYI_VIBC3
AccessioniPrimary (citable) accession number: A5F8Z3
Secondary accession number(s): C3LXZ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: June 12, 2007
Last modified: May 14, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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