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Protein

Fumarate and nitrate reduction regulatory protein

Gene

fnr

Organism
Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Global transcription factor that controls the expression of over 100 target genes in response to anoxia. It facilitates the adaptation to anaerobic growth conditions by regulating the expression of gene products that are involved in anaerobic energy metabolism. When the terminal electron acceptor, O2, is no longer available, it represses the synthesis of enzymes involved in aerobic respiration and increases the synthesis of enzymes required for anaerobic respiration (By similarity).By similarity

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi20Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi23Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi29Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi122Iron-sulfur (4Fe-4S)Sequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi200 – 219H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

4Fe-4S, DNA-binding, Iron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate and nitrate reduction regulatory protein
Gene namesi
Name:fnr
Ordered Locus Names:VC0395_A1045, VC395_1554
OrganismiVibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)
Taxonomic identifieri345073 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000249 Componenti: Chromosome 2

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003218531 – 250Fumarate and nitrate reduction regulatory proteinAdd BLAST250

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi345073.VC0395_A1045.

Structurei

3D structure databases

ProteinModelPortaliA5F890.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini164 – 237HTH crp-typePROSITE-ProRule annotationAdd BLAST74

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni23 – 32Essential for the oxygen-regulated activityBy similarity10
Regioni50 – 53Activating region 2ASequence analysis4
Regioni63 – 64Activating region 3ASequence analysis2
Regioni74 – 78Activating region 1ASequence analysis5
Regioni84Activating region 3BSequence analysis1
Regioni88 – 90Activating region 3CSequence analysis3
Regioni115Activating region 3DSequence analysis1
Regioni119 – 124Activating region 1BSequence analysis6
Regioni126 – 127Activating region 2BSequence analysis2
Regioni130 – 131Activating region 2CSequence analysis2
Regioni143 – 162DimerizationSequence analysisAdd BLAST20
Regioni184 – 194Activating region 1CSequence analysisAdd BLAST11

Sequence similaritiesi

Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation
Contains 1 HTH crp-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105DS0. Bacteria.
COG0664. LUCA.
HOGENOMiHOG000186461.
KOiK01420.
OMAiHVHKIMS.
OrthoDBiPOG091H0447.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP.
IPR014710. RmlC-like_jellyroll.
IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF13545. HTH_Crp_2. 1 hit.
[Graphical view]
PRINTSiPR00034. HTHCRP.
SMARTiSM00100. cNMP. 1 hit.
SM00419. HTH_CRP. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF51206. SSF51206. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 1 hit.
PS00042. HTH_CRP_1. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5F890-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISEKPAAKR IQSGGCAIHC QDCSISQLCI PFTLNESELD QLDQIIERKK
60 70 80 90 100
PIQKGQELFK AGDELRSLYA IRSGTIKSYT ITEQGDEQIT AFHLAGDLVG
110 120 130 140 150
FDAITGDQHP SFAQALETSM VCEIPYEILD DLSGKMPKLR QQIMRLMSNE
160 170 180 190 200
IKGDQEMILL LSKKNAEERL AAFLYNLSTR FSQRGFSPRE FRLTMTRGDI
210 220 230 240 250
GNYLGLTVET ISRLLGRFQK SEILSVKGKY ITILNHAELM ELAGVSKESK
Length:250
Mass (Da):28,071
Last modified:June 12, 2007 - v1
Checksum:iA3E3ED036BBBC490
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF244992 Genomic DNA. Translation: AAG09773.1.
CP000627 Genomic DNA. Translation: ABQ20599.1.
CP001235 Genomic DNA. Translation: ACP09560.1.
RefSeqiWP_000622582.1. NC_012582.1.

Genome annotation databases

EnsemblBacteriaiABQ20599; ABQ20599; VC0395_A1045.
ACP09560; ACP09560; VC395_1554.
KEGGivco:VC0395_A1045.
vcr:VC395_1554.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF244992 Genomic DNA. Translation: AAG09773.1.
CP000627 Genomic DNA. Translation: ABQ20599.1.
CP001235 Genomic DNA. Translation: ACP09560.1.
RefSeqiWP_000622582.1. NC_012582.1.

3D structure databases

ProteinModelPortaliA5F890.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi345073.VC0395_A1045.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ20599; ABQ20599; VC0395_A1045.
ACP09560; ACP09560; VC395_1554.
KEGGivco:VC0395_A1045.
vcr:VC395_1554.

Phylogenomic databases

eggNOGiENOG4105DS0. Bacteria.
COG0664. LUCA.
HOGENOMiHOG000186461.
KOiK01420.
OMAiHVHKIMS.
OrthoDBiPOG091H0447.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP.
IPR014710. RmlC-like_jellyroll.
IPR018335. Tscrpt_reg_HTH_Crp-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF13545. HTH_Crp_2. 1 hit.
[Graphical view]
PRINTSiPR00034. HTHCRP.
SMARTiSM00100. cNMP. 1 hit.
SM00419. HTH_CRP. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF51206. SSF51206. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 1 hit.
PS00042. HTH_CRP_1. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFNR_VIBC3
AccessioniPrimary (citable) accession number: A5F890
Secondary accession number(s): C3M0J4, Q9KS27
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: June 12, 2007
Last modified: September 7, 2016
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.