A5F7X7 (KATG_VIBC3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Synonyms:	perA
Ordered Locus Names:	VC0395_A1166, VC395_1677

Organism

Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) [Complete proteome] [HAMAP]

Taxonomic identifier

345073 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	724 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 724	724	Catalase-peroxidase HAMAP MF_01961		PRO_0000354948
Sites
	Active site	99	1	Proton acceptor By similarity
	Metal binding	267	1	Iron (heme axial ligand) By similarity
	Site	95	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	98 ↔ 226		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-252) By similarity
	Cross-link	226 ↔ 252		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-98) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5F7X7 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: C53832C812F6DB51	FASTA	724	80,681
	10 20 30 40 50 60 MEHNKAGSSG QCPVMHGGLT SASMSNMDWW PKALNLDILH QHDSKTNPLG ADFNYREELK 70 80 90 100 110 120 KLDVEALKRD LKALMTNSQE WWPADWGHYG GLMIRMAWHS AGTYRIADGR GGGGTGNQRF 130 140 150 160 170 180 APLNSWPDNA NLDKARRLLW PIKQKYGNKI SWADLMILAG NMAYESMGLK TFGFAFGRED 190 200 210 220 230 240 IWHPEKDIYW GSEKEWLAKS GGENSRYSGQ RDLENPLATV MMGLIYVNPE GVDGNPDPLK 250 260 270 280 290 300 TAQDMRVTFA RMAMNDEETV ALTAGGHTVG KAHGNGKASN LGPDPEGAEL HEQGLGWNNH 310 320 330 340 350 360 TSRGIGRNTV TSGIEGAWTT HPTRWDNEYF YLLLSYEWQL TKSPAGAWQW EPVNIKEEDK 370 380 390 400 410 420 PVDVEDPSIR YNPMMTDADM ALKIDPEYRK ISERFYKDPA YFSEVFARAW FKLTHRDMGP 430 440 450 460 470 480 KARYFGPDVP AEDLIWQDPV PAGRKDYDVN AVKAKIAASG LSISEMVSTA WDSARTFRGS 490 500 510 520 530 540 DKRGGANGAR IRLAPQKDWE GNEPARLGKV LAVLEKIAAE SGISIADTIV LAGNVGIEQA 550 560 570 580 590 600 AKAAGVNVTV PFAPGRGDAT IEQTDVESFE VLEPLADGFR NWQKKHYVVT PEEMLLDKAQ 610 620 630 640 650 660 LLRLTAPEMT VLIGGMRVLG TNYGGSQHGV FTDRVGALTN DFFVNLTDMS YTWKPTGRNS 670 680 690 700 710 720 YEIVERKSGK VKWTATRVDL VFGSNSILRA YAEVYAQDDN KEKFVKDFVA AWTKVMNADR FDLV

References

[1]	Heidelberg J. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39541 / Ogawa 395 / O395.
[2]	"A recalibrated molecular clock and independent origins for the cholera pandemic clones." Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J., Wang W., Wang J., Qian W., Li D., Wang L. PLoS ONE 3:E4053-E4053(2008) [PubMed: 19115014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39541 / Ogawa 395 / O395.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000627 Genomic DNA. Translation: ABQ20629.1. CP001235 Genomic DNA. Translation: ACP09682.1.
3D structure databases
ProteinModelPortal	A5F7X7.
SMR	A5F7X7. Positions 25-723.
ModBase	Search...
Protein-protein interaction databases
STRING	A5F7X7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GenomeReviews	Gene locus VC0395_A1166 in contig CP000627_GR. Gene locus VC395_1677 in contig CP001235_GR.
KEGG	vco:VC0395_A1166.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0376.
HOGENOM	HBG285610.
OMA	WPNALNL.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	VCHO345073:VC0395_A1166-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_VIBC3

Accession

Primary (citable) accession number: A5F7X7
Secondary accession number(s): C3M0W6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	June 12, 2007
Last modified:	November 16, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program