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A5F7A4

- NANH_VIBC3

UniProt

A5F7A4 - NANH_VIBC3

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Protein

Sialidase

Gene

nanH

Organism
Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Cleaves the terminal sialic acid (N-acetyl neuraminic acid) from carbohydrate chains in glycoproteins providing free sialic acid which can be used as carbon and energy sources. Sialidases have been suggested to be pathogenic factors in microbial infections. Facilitates cholera toxin binding to host intestinal epithelial cells by converting cell surface polysialogangliosides to GM1 monogangliosides.

Catalytic activityi

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Cofactori

Ca2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei224 – 2241SubstrateBy similarity
Active sitei250 – 2501Proton acceptorBy similarity
Active sitei619 – 6191Sequence Analysis
Binding sitei635 – 6351SubstrateBy similarity
Binding sitei712 – 7121SubstrateBy similarity
Active sitei740 – 7401NucleophileBy similarity

GO - Molecular functioni

  1. exo-alpha-(2->3)-sialidase activity Source: UniProtKB-EC
  2. exo-alpha-(2->6)-sialidase activity Source: UniProtKB-EC
  3. exo-alpha-(2->8)-sialidase activity Source: UniProtKB-EC
  4. sialic acid binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Calcium

Protein family/group databases

CAZyiCBM40. Carbohydrate-Binding Module Family 40.
GH33. Glycoside Hydrolase Family 33.

Names & Taxonomyi

Protein namesi
Recommended name:
Sialidase (EC:3.2.1.18)
Alternative name(s):
Neuraminidase
Short name:
NANase
Gene namesi
Name:nanH
Ordered Locus Names:VC0395_A1381, VC395_1898
OrganismiVibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)
Taxonomic identifieri345073 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000000249: Chromosome 2, UP000001630: Chromosome I

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 24241 PublicationAdd
BLAST
Chaini25 – 781757SialidasePRO_0000323013Add
BLAST

Expressioni

Inductioni

May be controlled by sialic acid availability and a growth-phase-dependent mechanism.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi345073.VC0395_A1381.

Structurei

3D structure databases

ProteinModelPortaliA5F7A4.
SMRiA5F7A4. Positions 25-781.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati263 – 27412BNR 1Add
BLAST
Repeati585 – 59612BNR 2Add
BLAST
Repeati653 – 66412BNR 3Add
BLAST
Repeati718 – 72912BNR 4Add
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 33 family.Curated
Contains 4 BNR repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiCOG4409.
HOGENOMiHOG000218204.
KOiK01186.

Family and domain databases

Gene3Di2.120.10.10. 2 hits.
2.60.120.200. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR026856. Sialidase_fam.
IPR011040. Sialidases.
IPR015344. VCNA_lectin-like_dom.
[Graphical view]
PANTHERiPTHR10628. PTHR10628. 1 hit.
PfamiPF09264. Sial-lect-inser. 2 hits.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 2 hits.
SSF50939. SSF50939. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A5F7A4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRFKNVKKTA LMLAMFGMAT SSNAALFDYN ATGDTEFDSP AKQGWMQDNT
60 70 80 90 100
NNGSGVLTNA DGMPAWLVQG IGGRAQWTYS LSTNQHAQAS SFGWRMTTEM
110 120 130 140 150
KVLSGGMITN YYANGTQRVL PIISLDSSGN LVVEFEGQTG RTVLATGTAA
160 170 180 190 200
TEYHKFELVF LPGSNPSASF YFDGKLIRDN IQPTASKQNM IVWGNGSSNT
210 220 230 240 250
DGVAAYRDIK FEIQGDVIFR GPDRIPSIVA SSVTPGVVTA FAEKRVGGGD
260 270 280 290 300
PGALSNTNDI ITRTSRDGGI TWDTELNLTE QINVSDEFDF SDPRPIYDPS
310 320 330 340 350
SNTVLVSYAR WPTDAAQNGD RIKPWMPNGI FYSVYDVASG NWQAPIDVTD
360 370 380 390 400
QVKERSFQIA GWGGSELYRR NTSLNSQQDW QSNAKIRIVD GAANQIQVAD
410 420 430 440 450
GSRKYVVTLS IDESGGLVAN LNGVSAPIIL QSEHAKVHSF HDYELQYSAL
460 470 480 490 500
NHTTTLFVDG QQITTWAGEV SQENNIQFGN ADAQIDGRLH VQKIVLTQQG
510 520 530 540 550
HNLVEFDAFY LAQQTPEVEK DLEKLGWTKI KTGNTMSLYG NASVNPGPGH
560 570 580 590 600
GITLTRQQNI SGSQNGRLIY PAIVLDRFFL NVMSIYSDDG GSNWQTGSTL
610 620 630 640 650
PIPFRWKSSS ILETLEPSEA DMVELQNGDL LLTARLDFNQ IVNGVNYSPR
660 670 680 690 700
QQFLSKDGGI TWSLLEANNA NVFSNISTGT VDASITRFEQ SDGSHFLLFT
710 720 730 740 750
NPQGNPAGTN GRQNLGLWFS FDEGVTWKGP IQLVNGASAY SDIYQLDSEN
760 770 780
AIVIVETDNS NMRILRMPIT LLKQKLTLSQ N
Length:781
Mass (Da):85,609
Last modified:March 18, 2008 - v2
Checksum:iFA85ED907FB20AF0
GO

Sequence cautioni

The sequence ABQ19703.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence ACP09894.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti22 – 221S → L in AAA27547. (PubMed:2832365)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M83562 Genomic DNA. Translation: AAA27546.1.
CP000627 Genomic DNA. Translation: ABQ19703.1. Different initiation.
CP001235 Genomic DNA. Translation: ACP09894.1. Different initiation.
M19268 Genomic DNA. Translation: AAA27547.1.
PIRiA27734.
A43866.
RefSeqiYP_001217324.1. NC_009457.1.
YP_002820130.1. NC_012582.1.

Genome annotation databases

EnsemblBacteriaiABQ19703; ABQ19703; VC0395_A1381.
ACP09894; ACP09894; VC395_1898.
GeneIDi5137230.
7775747.
KEGGivco:VC0395_A1381.
vcr:VC395_1898.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M83562 Genomic DNA. Translation: AAA27546.1 .
CP000627 Genomic DNA. Translation: ABQ19703.1 . Different initiation.
CP001235 Genomic DNA. Translation: ACP09894.1 . Different initiation.
M19268 Genomic DNA. Translation: AAA27547.1 .
PIRi A27734.
A43866.
RefSeqi YP_001217324.1. NC_009457.1.
YP_002820130.1. NC_012582.1.

3D structure databases

ProteinModelPortali A5F7A4.
SMRi A5F7A4. Positions 25-781.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 345073.VC0395_A1381.

Chemistry

BindingDBi A5F7A4.

Protein family/group databases

CAZyi CBM40. Carbohydrate-Binding Module Family 40.
GH33. Glycoside Hydrolase Family 33.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABQ19703 ; ABQ19703 ; VC0395_A1381 .
ACP09894 ; ACP09894 ; VC395_1898 .
GeneIDi 5137230.
7775747.
KEGGi vco:VC0395_A1381.
vcr:VC395_1898.

Phylogenomic databases

eggNOGi COG4409.
HOGENOMi HOG000218204.
KOi K01186.

Family and domain databases

Gene3Di 2.120.10.10. 2 hits.
2.60.120.200. 2 hits.
InterProi IPR013320. ConA-like_dom.
IPR026856. Sialidase_fam.
IPR011040. Sialidases.
IPR015344. VCNA_lectin-like_dom.
[Graphical view ]
PANTHERi PTHR10628. PTHR10628. 1 hit.
Pfami PF09264. Sial-lect-inser. 2 hits.
[Graphical view ]
SUPFAMi SSF49899. SSF49899. 2 hits.
SSF50939. SSF50939. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Role of Vibrio cholerae neuraminidase in the function of cholera toxin."
    Galen J.E., Ketley J.M., Fasano A., Richardson S.H., Wasserman S.S., Kaper J.B.
    Infect. Immun. 60:406-415(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Heidelberg J.
    Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39541 / Classical Ogawa 395 / O395.
  3. "A recalibrated molecular clock and independent origins for the cholera pandemic clones."
    Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J., Wang W., Wang J., Qian W., Li D., Wang L.
    PLoS ONE 3:E4053-E4053(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39541 / Classical Ogawa 395 / O395.
  4. "Cloning and expression of the Vibrio cholerae neuraminidase gene nanH in Escherichia coli."
    Vimr E.R., Lawrisuk L., Galen J.E., Kaper J.B.
    J. Bacteriol. 170:1495-1504(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-44, PROTEIN SEQUENCE OF 25-44.

Entry informationi

Entry nameiNANH_VIBC3
AccessioniPrimary (citable) accession number: A5F7A4
Secondary accession number(s): C3M1H8, P37060, Q9KR59
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: November 26, 2014
This is version 52 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3