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Protein

Sialidase

Gene

nanH

Organism
Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves the terminal sialic acid (N-acetyl neuraminic acid) from carbohydrate chains in glycoproteins providing free sialic acid which can be used as carbon and energy sources. Sialidases have been suggested to be pathogenic factors in microbial infections. Facilitates cholera toxin binding to host intestinal epithelial cells by converting cell surface polysialogangliosides to GM1 monogangliosides.

Catalytic activityi

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Cofactori

Ca2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei224SubstrateBy similarity1
Active sitei250Proton acceptorBy similarity1
Active sitei619Sequence analysis1
Binding sitei635SubstrateBy similarity1
Binding sitei712SubstrateBy similarity1
Active sitei740NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Calcium

Protein family/group databases

CAZyiCBM40. Carbohydrate-Binding Module Family 40.
GH33. Glycoside Hydrolase Family 33.

Names & Taxonomyi

Protein namesi
Recommended name:
Sialidase (EC:3.2.1.18)
Alternative name(s):
Neuraminidase
Short name:
NANase
Gene namesi
Name:nanH
Ordered Locus Names:VC0395_A1381, VC395_1898
OrganismiVibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)
Taxonomic identifieri345073 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000249 Componenti: Chromosome 2

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 241 PublicationAdd BLAST24
ChainiPRO_000032301325 – 781SialidaseAdd BLAST757

Expressioni

Inductioni

May be controlled by sialic acid availability and a growth-phase-dependent mechanism.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi345073.VC0395_A1381.

Structurei

3D structure databases

ProteinModelPortaliA5F7A4.
SMRiA5F7A4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati263 – 274BNR 1Add BLAST12
Repeati585 – 596BNR 2Add BLAST12
Repeati653 – 664BNR 3Add BLAST12
Repeati718 – 729BNR 4Add BLAST12

Sequence similaritiesi

Belongs to the glycosyl hydrolase 33 family.Curated
Contains 4 BNR repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG4108478. Bacteria.
COG4409. LUCA.
HOGENOMiHOG000218204.
KOiK01186.
OrthoDBiPOG091H0EBR.

Family and domain databases

Gene3Di2.120.10.10. 2 hits.
2.60.120.200. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR026856. Sialidase_fam.
IPR011040. Sialidases.
IPR015344. VCNA_lectin-like_dom.
[Graphical view]
PANTHERiPTHR10628. PTHR10628. 2 hits.
PfamiPF13088. BNR_2. 1 hit.
PF09264. Sial-lect-inser. 2 hits.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 2 hits.
SSF50939. SSF50939. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A5F7A4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFKNVKKTA LMLAMFGMAT SSNAALFDYN ATGDTEFDSP AKQGWMQDNT
60 70 80 90 100
NNGSGVLTNA DGMPAWLVQG IGGRAQWTYS LSTNQHAQAS SFGWRMTTEM
110 120 130 140 150
KVLSGGMITN YYANGTQRVL PIISLDSSGN LVVEFEGQTG RTVLATGTAA
160 170 180 190 200
TEYHKFELVF LPGSNPSASF YFDGKLIRDN IQPTASKQNM IVWGNGSSNT
210 220 230 240 250
DGVAAYRDIK FEIQGDVIFR GPDRIPSIVA SSVTPGVVTA FAEKRVGGGD
260 270 280 290 300
PGALSNTNDI ITRTSRDGGI TWDTELNLTE QINVSDEFDF SDPRPIYDPS
310 320 330 340 350
SNTVLVSYAR WPTDAAQNGD RIKPWMPNGI FYSVYDVASG NWQAPIDVTD
360 370 380 390 400
QVKERSFQIA GWGGSELYRR NTSLNSQQDW QSNAKIRIVD GAANQIQVAD
410 420 430 440 450
GSRKYVVTLS IDESGGLVAN LNGVSAPIIL QSEHAKVHSF HDYELQYSAL
460 470 480 490 500
NHTTTLFVDG QQITTWAGEV SQENNIQFGN ADAQIDGRLH VQKIVLTQQG
510 520 530 540 550
HNLVEFDAFY LAQQTPEVEK DLEKLGWTKI KTGNTMSLYG NASVNPGPGH
560 570 580 590 600
GITLTRQQNI SGSQNGRLIY PAIVLDRFFL NVMSIYSDDG GSNWQTGSTL
610 620 630 640 650
PIPFRWKSSS ILETLEPSEA DMVELQNGDL LLTARLDFNQ IVNGVNYSPR
660 670 680 690 700
QQFLSKDGGI TWSLLEANNA NVFSNISTGT VDASITRFEQ SDGSHFLLFT
710 720 730 740 750
NPQGNPAGTN GRQNLGLWFS FDEGVTWKGP IQLVNGASAY SDIYQLDSEN
760 770 780
AIVIVETDNS NMRILRMPIT LLKQKLTLSQ N
Length:781
Mass (Da):85,609
Last modified:March 18, 2008 - v2
Checksum:iFA85ED907FB20AF0
GO

Sequence cautioni

The sequence ABQ19703 differs from that shown. Reason: Erroneous initiation.Curated
The sequence ACP09894 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti22S → L in AAA27547 (PubMed:2832365).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M83562 Genomic DNA. Translation: AAA27546.1.
CP000627 Genomic DNA. Translation: ABQ19703.1. Different initiation.
CP001235 Genomic DNA. Translation: ACP09894.1. Different initiation.
M19268 Genomic DNA. Translation: AAA27547.1.
PIRiA27734.
A43866.
RefSeqiWP_001211290.1. NC_012582.1.

Genome annotation databases

EnsemblBacteriaiABQ19703; ABQ19703; VC0395_A1381.
ACP09894; ACP09894; VC395_1898.
KEGGivco:VC0395_A1381.
vcr:VC395_1898.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M83562 Genomic DNA. Translation: AAA27546.1.
CP000627 Genomic DNA. Translation: ABQ19703.1. Different initiation.
CP001235 Genomic DNA. Translation: ACP09894.1. Different initiation.
M19268 Genomic DNA. Translation: AAA27547.1.
PIRiA27734.
A43866.
RefSeqiWP_001211290.1. NC_012582.1.

3D structure databases

ProteinModelPortaliA5F7A4.
SMRiA5F7A4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi345073.VC0395_A1381.

Protein family/group databases

CAZyiCBM40. Carbohydrate-Binding Module Family 40.
GH33. Glycoside Hydrolase Family 33.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ19703; ABQ19703; VC0395_A1381.
ACP09894; ACP09894; VC395_1898.
KEGGivco:VC0395_A1381.
vcr:VC395_1898.

Phylogenomic databases

eggNOGiENOG4108478. Bacteria.
COG4409. LUCA.
HOGENOMiHOG000218204.
KOiK01186.
OrthoDBiPOG091H0EBR.

Family and domain databases

Gene3Di2.120.10.10. 2 hits.
2.60.120.200. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR026856. Sialidase_fam.
IPR011040. Sialidases.
IPR015344. VCNA_lectin-like_dom.
[Graphical view]
PANTHERiPTHR10628. PTHR10628. 2 hits.
PfamiPF13088. BNR_2. 1 hit.
PF09264. Sial-lect-inser. 2 hits.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 2 hits.
SSF50939. SSF50939. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiNANH_VIBC3
AccessioniPrimary (citable) accession number: A5F7A4
Secondary accession number(s): C3M1H8, P37060, Q9KR59
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: November 2, 2016
This is version 68 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.