A5F5X3 (RLMG_VIBC3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase G
EC=2.1.1.174

Alternative name(s):
23S rRNA m2G1835 methyltransferase
rRNA (guanine-N(2)-)-methyltransferase RlmG

Gene names

Name:	rlmG
Ordered Locus Names:	VC0395_A1886, VC395_2413

Organism

Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) [Complete proteome] [HAMAP]

Taxonomic identifier

345073 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio › Vibrio cholerae

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the guanine in position 1835 (m2G1835) of 23S rRNA By similarity. HAMAP-Rule MF_01859
Catalytic activity	S-adenosyl-L-methionine + guanine(1835) in 23S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanine(1835) in 23S rRNA. HAMAP-Rule MF_01859
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_01859.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmG family.
Sequence caution	The sequence ABQ21539.1 differs from that shown. Reason: Erroneous initiation. The sequence ACP10403.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	23S rRNA (guanine(1835)-N(2))-methyltransferase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Ribosomal RNA large subunit methyltransferase G HAMAP-Rule MF_01859		PRO_0000366531

Sequences

	Sequence		Length	Mass (Da)
	A5F5X3 [UniParc]. Last modified March 24, 2009. Version 2. Checksum: FB166ECA68CBA65F	FASTA	376	42,051
	10 20 30 40 50 60 MKTELTLLEQ TLTLHRFPKR NNETLQAWDA GDEYLIQHVE QLALPESSHI VIINDHFGTL 70 80 90 100 110 120 SCWFSQKHKV SMMSDSYIAH QATQANLQQN QRPPVQLLTT LDPVPNDASV VLLQLPKSNR 130 140 150 160 170 180 HLVWILSQLR KALSPNIPII AVNKAKEIHT STLNLFEKHL GPTTTSLAWK KHRLVFSSAT 190 200 210 220 230 240 VNPANEVNPE CGWSIEPYAI TLTNLPNVYS GESLDLGSRF ILEHLPADPT LEDFIDLGCG 250 260 270 280 290 300 NGVLSVRLGQ LNPQAKITCV DESFMAIASA QKNLHDNLGK RDIHCIANNC LDGFPAHSSS 310 320 330 340 350 360 MIVCNPPFHQ QQTITDHIAW QMFCDSKHVL KKGGKLWVIG NRHLGYDVKL ARLFGKSHVR 370 VIANNSKFVI LQAIKS

References

[1]	Heidelberg J. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39541 / Ogawa 395 / O395.
[2]	"A recalibrated molecular clock and independent origins for the cholera pandemic clones." Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J., Wang W., Wang J., Qian W., Li D., Wang L. PLoS ONE 3:E4053-E4053(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39541 / Ogawa 395 / O395.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000627 Genomic DNA. Translation: ABQ21539.1. Different initiation. CP001235 Genomic DNA. Translation: ACP10403.1. Different initiation.
RefSeq	YP_001217810.1. NC_009457.1. YP_002820639.1. NC_012582.1.
3D structure databases
ProteinModelPortal	A5F5X3.
ModBase	Search...
Protein-protein interaction databases
STRING	345073.VC0395_A1886.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABQ21539; ABQ21539; VC0395_A1886. ACP10403; ACP10403; VC395_2413.
GeneID	5135892. 7776188.
KEGG	vco:VC0395_A1886. vcr:VC395_2413.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2813.
HOGENOM	HOG000246323.
KO	K00564.
ProtClustDB	CLSK874746.
Family and domain databases
HAMAP	MF_01859. 23SrRNA_methyltr_G.
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR017237. rRNA_m2G-MeTrfase_RsmD. IPR007848. Small_mtfrase_dom. [Graphical view]
Pfam	PF05175. MTS. 1 hit. [Graphical view]
PIRSF	PIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMG_VIBC3

Accession

Primary (citable) accession number: A5F5X3
Secondary accession number(s): C3M421

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	March 24, 2009
Last modified:	May 1, 2013
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program