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Protein

Beta-galactosidase

Gene

lacZ

Organism
Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 2 magnesium ions per monomer.UniRule annotation
  • Na(+)UniRule annotationNote: Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei100 – 1001SubstrateUniRule annotation
Metal bindingi198 – 1981SodiumUniRule annotation
Binding sitei198 – 1981SubstrateUniRule annotation
Sitei355 – 3551Transition state stabilizerUniRule annotation
Sitei389 – 3891Transition state stabilizerUniRule annotation
Metal bindingi414 – 4141Magnesium 1UniRule annotation
Metal bindingi416 – 4161Magnesium 1UniRule annotation
Active sitei459 – 4591Proton donorUniRule annotation
Metal bindingi459 – 4591Magnesium 1UniRule annotation
Binding sitei459 – 4591SubstrateUniRule annotation
Active sitei535 – 5351NucleophileUniRule annotation
Metal bindingi595 – 5951Magnesium 2UniRule annotation
Metal bindingi599 – 5991Sodium; via carbonyl oxygenUniRule annotation
Metal bindingi602 – 6021SodiumUniRule annotation
Binding sitei602 – 6021SubstrateUniRule annotation
Binding sitei995 – 9951SubstrateUniRule annotation

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
LactaseUniRule annotation
Gene namesi
Name:lacZUniRule annotation
Ordered Locus Names:VC0395_A1917, VC395_2453
OrganismiVibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)
Taxonomic identifieri345073 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000000249 Componenti: Chromosome 2 UP000001630 Componenti: Chromosome I

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10241024Beta-galactosidasePRO_0000367011Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi345073.VC0395_A1917.

Structurei

3D structure databases

ProteinModelPortaliA5F5U6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni535 – 5384Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000252443.
KOiK01190.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5F5U6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRNFSDILLS QDWQNPHIVK WHCRTPHVPL HSYRTEQEAR LDVGGNRQSL
60 70 80 90 100
NGQWRFALFE KPEAVEPAVI DPDFDDSAWA HIPVPSNWQM QGFDKPIYTN
110 120 130 140 150
IQYPFADRPP YVPQDNPTGC YRHRFTLEKQ ALTESIRIVF DGVNSAFHLW
160 170 180 190 200
CNGHWVGYSQ DSRLPAEFEL TPYLQEGENL LVAMVLRWSD GSYLEDQDMW
210 220 230 240 250
WLSGIFRDVY LYRKPILAIE DFFIRTELDA LYQHAELRVE TRLSQVTRHH
260 270 280 290 300
QVQVALFDAQ GECVARSQAL HTGQRVVDEK GAWHDKTEHS LAICSPTLWS
310 320 330 340 350
DEAPYLYRCV ICLLDEDGAP IEFESAAVGF RKVEITQGLL KLNGQPLLIR
360 370 380 390 400
GVNRHEHHPE LGHVMDEASM RRDIELMKQH NFNAVRTAHY PNHPRWYELC
410 420 430 440 450
DEYGLYVVDE ANLETHGQFP MSRLSNDPQW VNAYLQRMIG MVERDKNHPC
460 470 480 490 500
VIIWSLGNES GIGTNHHAMY QWTKQRDPSR PVQYEGGGAN TAATDIVCPM
510 520 530 540 550
YARVDQHQPH PAVPKYALKN WISLPQENRP LILCEYAHAM GNSLGAFYKY
560 570 580 590 600
WQAFREFPRL QGGFIWDWVD QGISKWDSEG RHYWGYGGDF GDTINDRQFC
610 620 630 640 650
INGLLFPDRT PHPALHEVKK VQQPYQFSLS YPKLTIHNER LFAALPLELV
660 670 680 690 700
VSVLCDGQEI KQERLPLDIA PRGTITLDLA SLPMLPEHEY HLNAVLLCRE
710 720 730 740 750
DQPWSNAGHC IASEQWCLQP RRSMLPKITH APLPQWQQDG DKVRIEAANQ
760 770 780 790 800
QWQFNRQTGL LEQWWQNGQP VLSEPLRDNF YRAVLDNDIG TSEAQHLDPN
810 820 830 840 850
SWIARWHAAG LDKLRVECDD LRVTTLNESV EVVIDVAHYH QQALALRTRW
860 870 880 890 900
RYQIFGDARV ELNVEVMLCS DLPPLPRVGL TLALPVAENP VSWFGRGPHE
910 920 930 940 950
NYPDRLQSAH VGRYTATVDE LHTPYIFPSE NGLRCDTRQL QVGALVVEGH
960 970 980 990 1000
FHFSLSRYSQ TMLDKAKHSN ELVAGDKWYL NLDAQHMGVG GDDSWSQSVH
1010 1020
PEFLLTQPHY QYQLTLRVKA SSPQ
Length:1,024
Mass (Da):118,176
Last modified:March 23, 2009 - v2
Checksum:i204F11E6188E33D8
GO

Sequence cautioni

The sequence ABQ20296.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence ACP10443.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000627 Genomic DNA. Translation: ABQ20296.1. Different initiation.
CP001235 Genomic DNA. Translation: ACP10443.1. Different initiation.
RefSeqiYP_001217841.1. NC_009457.1.
YP_002820679.1. NC_012582.1.

Genome annotation databases

EnsemblBacteriaiABQ20296; ABQ20296; VC0395_A1917.
ACP10443; ACP10443; VC395_2453.
GeneIDi5135797.
7776226.
KEGGivco:VC0395_A1917.
vcr:VC395_2453.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000627 Genomic DNA. Translation: ABQ20296.1. Different initiation.
CP001235 Genomic DNA. Translation: ACP10443.1. Different initiation.
RefSeqiYP_001217841.1. NC_009457.1.
YP_002820679.1. NC_012582.1.

3D structure databases

ProteinModelPortaliA5F5U6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi345073.VC0395_A1917.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ20296; ABQ20296; VC0395_A1917.
ACP10443; ACP10443; VC395_2453.
GeneIDi5135797.
7776226.
KEGGivco:VC0395_A1917.
vcr:VC395_2453.

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000252443.
KOiK01190.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Heidelberg J.
    Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39541 / Classical Ogawa 395 / O395.
  2. "A recalibrated molecular clock and independent origins for the cholera pandemic clones."
    Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J., Wang W., Wang J., Qian W., Li D., Wang L.
    PLoS ONE 3:E4053-E4053(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39541 / Classical Ogawa 395 / O395.

Entry informationi

Entry nameiBGAL_VIBC3
AccessioniPrimary (citable) accession number: A5F5U6
Secondary accession number(s): C3M461
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 23, 2009
Last sequence update: March 23, 2009
Last modified: January 6, 2015
This is version 50 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.