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Protein

Beta-galactosidase

Gene

lacZ

Organism
Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 2 magnesium ions per monomer.UniRule annotation
  • Na+UniRule annotationNote: Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei100SubstrateUniRule annotation1
Metal bindingi198SodiumUniRule annotation1
Binding sitei198SubstrateUniRule annotation1
Sitei355Transition state stabilizerUniRule annotation1
Sitei389Transition state stabilizerUniRule annotation1
Metal bindingi414Magnesium 1UniRule annotation1
Metal bindingi416Magnesium 1UniRule annotation1
Active sitei459Proton donorUniRule annotation1
Metal bindingi459Magnesium 1UniRule annotation1
Binding sitei459SubstrateUniRule annotation1
Active sitei535NucleophileUniRule annotation1
Metal bindingi595Magnesium 2UniRule annotation1
Metal bindingi599Sodium; via carbonyl oxygenUniRule annotation1
Metal bindingi602SodiumUniRule annotation1
Binding sitei602SubstrateUniRule annotation1
Binding sitei995SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
LactaseUniRule annotation
Gene namesi
Name:lacZUniRule annotation
Ordered Locus Names:VC0395_A1917, VC395_2453
OrganismiVibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)
Taxonomic identifieri345073 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000249 Componenti: Chromosome 2

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003670111 – 1024Beta-galactosidaseAdd BLAST1024

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi345073.VC0395_A1917.

Structurei

3D structure databases

ProteinModelPortaliA5F5U6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni535 – 538Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CNT. Bacteria.
COG3250. LUCA.
HOGENOMiHOG000252443.
KOiK01190.
OrthoDBiPOG091H07QB.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. GH-type_carb-bd.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006103. Glyco_hydro_2_cat.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR032312. LacZ_4.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF16353. DUF4981. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5F5U6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRNFSDILLS QDWQNPHIVK WHCRTPHVPL HSYRTEQEAR LDVGGNRQSL
60 70 80 90 100
NGQWRFALFE KPEAVEPAVI DPDFDDSAWA HIPVPSNWQM QGFDKPIYTN
110 120 130 140 150
IQYPFADRPP YVPQDNPTGC YRHRFTLEKQ ALTESIRIVF DGVNSAFHLW
160 170 180 190 200
CNGHWVGYSQ DSRLPAEFEL TPYLQEGENL LVAMVLRWSD GSYLEDQDMW
210 220 230 240 250
WLSGIFRDVY LYRKPILAIE DFFIRTELDA LYQHAELRVE TRLSQVTRHH
260 270 280 290 300
QVQVALFDAQ GECVARSQAL HTGQRVVDEK GAWHDKTEHS LAICSPTLWS
310 320 330 340 350
DEAPYLYRCV ICLLDEDGAP IEFESAAVGF RKVEITQGLL KLNGQPLLIR
360 370 380 390 400
GVNRHEHHPE LGHVMDEASM RRDIELMKQH NFNAVRTAHY PNHPRWYELC
410 420 430 440 450
DEYGLYVVDE ANLETHGQFP MSRLSNDPQW VNAYLQRMIG MVERDKNHPC
460 470 480 490 500
VIIWSLGNES GIGTNHHAMY QWTKQRDPSR PVQYEGGGAN TAATDIVCPM
510 520 530 540 550
YARVDQHQPH PAVPKYALKN WISLPQENRP LILCEYAHAM GNSLGAFYKY
560 570 580 590 600
WQAFREFPRL QGGFIWDWVD QGISKWDSEG RHYWGYGGDF GDTINDRQFC
610 620 630 640 650
INGLLFPDRT PHPALHEVKK VQQPYQFSLS YPKLTIHNER LFAALPLELV
660 670 680 690 700
VSVLCDGQEI KQERLPLDIA PRGTITLDLA SLPMLPEHEY HLNAVLLCRE
710 720 730 740 750
DQPWSNAGHC IASEQWCLQP RRSMLPKITH APLPQWQQDG DKVRIEAANQ
760 770 780 790 800
QWQFNRQTGL LEQWWQNGQP VLSEPLRDNF YRAVLDNDIG TSEAQHLDPN
810 820 830 840 850
SWIARWHAAG LDKLRVECDD LRVTTLNESV EVVIDVAHYH QQALALRTRW
860 870 880 890 900
RYQIFGDARV ELNVEVMLCS DLPPLPRVGL TLALPVAENP VSWFGRGPHE
910 920 930 940 950
NYPDRLQSAH VGRYTATVDE LHTPYIFPSE NGLRCDTRQL QVGALVVEGH
960 970 980 990 1000
FHFSLSRYSQ TMLDKAKHSN ELVAGDKWYL NLDAQHMGVG GDDSWSQSVH
1010 1020
PEFLLTQPHY QYQLTLRVKA SSPQ
Length:1,024
Mass (Da):118,176
Last modified:March 24, 2009 - v2
Checksum:i204F11E6188E33D8
GO

Sequence cautioni

The sequence ABQ20296 differs from that shown. Reason: Erroneous initiation.Curated
The sequence ACP10443 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000627 Genomic DNA. Translation: ABQ20296.1. Different initiation.
CP001235 Genomic DNA. Translation: ACP10443.1. Different initiation.
RefSeqiWP_001243585.1. NC_012582.1.

Genome annotation databases

EnsemblBacteriaiABQ20296; ABQ20296; VC0395_A1917.
ACP10443; ACP10443; VC395_2453.
KEGGivco:VC0395_A1917.
vcr:VC395_2453.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000627 Genomic DNA. Translation: ABQ20296.1. Different initiation.
CP001235 Genomic DNA. Translation: ACP10443.1. Different initiation.
RefSeqiWP_001243585.1. NC_012582.1.

3D structure databases

ProteinModelPortaliA5F5U6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi345073.VC0395_A1917.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ20296; ABQ20296; VC0395_A1917.
ACP10443; ACP10443; VC395_2453.
KEGGivco:VC0395_A1917.
vcr:VC395_2453.

Phylogenomic databases

eggNOGiENOG4105CNT. Bacteria.
COG3250. LUCA.
HOGENOMiHOG000252443.
KOiK01190.
OrthoDBiPOG091H07QB.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. GH-type_carb-bd.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006103. Glyco_hydro_2_cat.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR032312. LacZ_4.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF16353. DUF4981. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBGAL_VIBC3
AccessioniPrimary (citable) accession number: A5F5U6
Secondary accession number(s): C3M461
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: March 24, 2009
Last modified: September 7, 2016
This is version 57 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.