Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

A5F5U6

- BGAL_VIBC3

UniProt

A5F5U6 - BGAL_VIBC3

Protein

Beta-galactosidase

Gene

lacZ

Organism
Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 48 (01 Oct 2014)
      Sequence version 2 (24 Mar 2009)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

    Cofactori

    Binds 2 magnesium ions per monomer.UniRule annotation
    Binds 1 sodium ion per monomer.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei100 – 1001SubstrateUniRule annotation
    Metal bindingi198 – 1981SodiumUniRule annotation
    Binding sitei198 – 1981SubstrateUniRule annotation
    Sitei355 – 3551Transition state stabilizerUniRule annotation
    Sitei389 – 3891Transition state stabilizerUniRule annotation
    Metal bindingi414 – 4141Magnesium 1UniRule annotation
    Metal bindingi416 – 4161Magnesium 1UniRule annotation
    Active sitei459 – 4591Proton donorUniRule annotation
    Metal bindingi459 – 4591Magnesium 1UniRule annotation
    Binding sitei459 – 4591SubstrateUniRule annotation
    Active sitei535 – 5351NucleophileUniRule annotation
    Metal bindingi595 – 5951Magnesium 2UniRule annotation
    Metal bindingi599 – 5991Sodium; via carbonyl oxygenUniRule annotation
    Metal bindingi602 – 6021SodiumUniRule annotation
    Binding sitei602 – 6021SubstrateUniRule annotation
    Binding sitei995 – 9951SubstrateUniRule annotation

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Sodium

    Protein family/group databases

    CAZyiGH2. Glycoside Hydrolase Family 2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
    Short name:
    Beta-galUniRule annotation
    Alternative name(s):
    LactaseUniRule annotation
    Gene namesi
    Name:lacZUniRule annotation
    Ordered Locus Names:VC0395_A1917, VC395_2453
    OrganismiVibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)
    Taxonomic identifieri345073 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
    ProteomesiUP000000249: Chromosome 2, UP000001630: Chromosome I

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10241024Beta-galactosidasePRO_0000367011Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi345073.VC0395_A1917.

    Structurei

    3D structure databases

    ProteinModelPortaliA5F5U6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni535 – 5384Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 2 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG3250.
    HOGENOMiHOG000252443.
    KOiK01190.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPiMF_01687. Beta_gal.
    InterProiIPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    PRINTSiPR00132. GLHYDRLASE2.
    SMARTiSM01038. Bgal_small_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A5F5U6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRNFSDILLS QDWQNPHIVK WHCRTPHVPL HSYRTEQEAR LDVGGNRQSL     50
    NGQWRFALFE KPEAVEPAVI DPDFDDSAWA HIPVPSNWQM QGFDKPIYTN 100
    IQYPFADRPP YVPQDNPTGC YRHRFTLEKQ ALTESIRIVF DGVNSAFHLW 150
    CNGHWVGYSQ DSRLPAEFEL TPYLQEGENL LVAMVLRWSD GSYLEDQDMW 200
    WLSGIFRDVY LYRKPILAIE DFFIRTELDA LYQHAELRVE TRLSQVTRHH 250
    QVQVALFDAQ GECVARSQAL HTGQRVVDEK GAWHDKTEHS LAICSPTLWS 300
    DEAPYLYRCV ICLLDEDGAP IEFESAAVGF RKVEITQGLL KLNGQPLLIR 350
    GVNRHEHHPE LGHVMDEASM RRDIELMKQH NFNAVRTAHY PNHPRWYELC 400
    DEYGLYVVDE ANLETHGQFP MSRLSNDPQW VNAYLQRMIG MVERDKNHPC 450
    VIIWSLGNES GIGTNHHAMY QWTKQRDPSR PVQYEGGGAN TAATDIVCPM 500
    YARVDQHQPH PAVPKYALKN WISLPQENRP LILCEYAHAM GNSLGAFYKY 550
    WQAFREFPRL QGGFIWDWVD QGISKWDSEG RHYWGYGGDF GDTINDRQFC 600
    INGLLFPDRT PHPALHEVKK VQQPYQFSLS YPKLTIHNER LFAALPLELV 650
    VSVLCDGQEI KQERLPLDIA PRGTITLDLA SLPMLPEHEY HLNAVLLCRE 700
    DQPWSNAGHC IASEQWCLQP RRSMLPKITH APLPQWQQDG DKVRIEAANQ 750
    QWQFNRQTGL LEQWWQNGQP VLSEPLRDNF YRAVLDNDIG TSEAQHLDPN 800
    SWIARWHAAG LDKLRVECDD LRVTTLNESV EVVIDVAHYH QQALALRTRW 850
    RYQIFGDARV ELNVEVMLCS DLPPLPRVGL TLALPVAENP VSWFGRGPHE 900
    NYPDRLQSAH VGRYTATVDE LHTPYIFPSE NGLRCDTRQL QVGALVVEGH 950
    FHFSLSRYSQ TMLDKAKHSN ELVAGDKWYL NLDAQHMGVG GDDSWSQSVH 1000
    PEFLLTQPHY QYQLTLRVKA SSPQ 1024
    Length:1,024
    Mass (Da):118,176
    Last modified:March 24, 2009 - v2
    Checksum:i204F11E6188E33D8
    GO

    Sequence cautioni

    The sequence ABQ20296.1 differs from that shown. Reason: Erroneous initiation.
    The sequence ACP10443.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000627 Genomic DNA. Translation: ABQ20296.1. Different initiation.
    CP001235 Genomic DNA. Translation: ACP10443.1. Different initiation.
    RefSeqiYP_001217841.1. NC_009457.1.
    YP_002820679.1. NC_012582.1.

    Genome annotation databases

    EnsemblBacteriaiABQ20296; ABQ20296; VC0395_A1917.
    ACP10443; ACP10443; VC395_2453.
    GeneIDi5135797.
    7776226.
    KEGGivco:VC0395_A1917.
    vcr:VC395_2453.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000627 Genomic DNA. Translation: ABQ20296.1 . Different initiation.
    CP001235 Genomic DNA. Translation: ACP10443.1 . Different initiation.
    RefSeqi YP_001217841.1. NC_009457.1.
    YP_002820679.1. NC_012582.1.

    3D structure databases

    ProteinModelPortali A5F5U6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 345073.VC0395_A1917.

    Protein family/group databases

    CAZyi GH2. Glycoside Hydrolase Family 2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABQ20296 ; ABQ20296 ; VC0395_A1917 .
    ACP10443 ; ACP10443 ; VC395_2453 .
    GeneIDi 5135797.
    7776226.
    KEGGi vco:VC0395_A1917.
    vcr:VC395_2453.

    Phylogenomic databases

    eggNOGi COG3250.
    HOGENOMi HOG000252443.
    KOi K01190.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPi MF_01687. Beta_gal.
    InterProi IPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00132. GLHYDRLASE2.
    SMARTi SM01038. Bgal_small_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Heidelberg J.
      Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 39541 / Classical Ogawa 395 / O395.
    2. "A recalibrated molecular clock and independent origins for the cholera pandemic clones."
      Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J., Wang W., Wang J., Qian W., Li D., Wang L.
      PLoS ONE 3:E4053-E4053(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 39541 / Classical Ogawa 395 / O395.

    Entry informationi

    Entry nameiBGAL_VIBC3
    AccessioniPrimary (citable) accession number: A5F5U6
    Secondary accession number(s): C3M461
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: March 24, 2009
    Last modified: October 1, 2014
    This is version 48 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3