A5F5C9 (PYRB_VIBC3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate carbamoyltransferase
EC=2.1.3.2

Alternative name(s):
Aspartate transcarbamylase
Short name=ATCase

Gene names

Name:	pyrB
Ordered Locus Names:	VC0395_A2092, VC395_2624

Organism

Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) [Complete proteome] [HAMAP]

Taxonomic identifier

345073 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate. HAMAP MF_00001
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3. HAMAP MF_00001
Sequence similarities	Belongs to the ATCase/OTCase family.
Sequence caution	The sequence ABQ21919.1 differs from that shown. Reason: Erroneous initiation. The sequence ACP10610.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro aspartate carbamoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	Aspartate carbamoyltransferase HAMAP MF_00001		PRO_0000321181

Sequences

	Sequence		Length	Mass (Da)
	A5F5C9 [UniParc]. Last modified February 26, 2008. Version 2. Checksum: AD073EE793872BA5	FASTA	309	34,350
	10 20 30 40 50 60 MANSLYQKHI ISIAELSRAE LELIVKTAGQ LKAQPNPELI KHKVVASCFF EPSTRTRLSF 70 80 90 100 110 120 ETAIQRIGGS VIGFDNGGNT SLAKKGETLA DSVRVISSYV DAFVMRHPQE GAARLASEFS 130 140 150 160 170 180 NGIPVINAGD GSNQHPSQTL LDLYSIFETQ GRLDNLDVAF VGDLKYGRTV HSLAQALAKF 190 200 210 220 230 240 DNNRFYFVAP EALAMPDYIC EELDEAGVKY QVFSDMESVI PELDILYMTR VQKERFDESE 250 260 270 280 290 300 YAHIKSAYIL TAAHLSDARS NLKVLHPLPR VDEITTDVDK TPHAYYFEQV ENGVYAREAL LALVLNESL

References

[1]	Heidelberg J. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39541 / Ogawa 395 / O395.
[2]	"A recalibrated molecular clock and independent origins for the cholera pandemic clones." Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J., Wang W., Wang J., Qian W., Li D., Wang L. PLoS ONE 3:E4053-E4053(2008) [PubMed: 19115014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39541 / Ogawa 395 / O395.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000627 Genomic DNA. Translation: ABQ21919.1. Different initiation. CP001235 Genomic DNA. Translation: ACP10610.1. Different initiation.
3D structure databases
ProteinModelPortal	A5F5C9.
SMR	A5F5C9. Positions 2-309.
ModBase	Search...
Protein-protein interaction databases
STRING	A5F5C9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GenomeReviews	Gene locus VC0395_A2092 in contig CP000627_GR. Gene locus VC395_2624 in contig CP001235_GR.
KEGG	vco:VC0395_A2092.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0540.
HOGENOM	HBG579429.
ProtClustDB	PRK00856.
Enzyme and pathway databases
BioCyc	VCHO345073:VC0395_A2092-MONOMER.
Family and domain databases
HAMAP	MF_00001. Asp_carb_tr. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. IPR002082. Asp_carbamoyltransf_euk. [Graphical view]
KO	K00609.
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00101. ATCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00670. Asp_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRB_VIBC3

Accession

Primary (citable) accession number: A5F5C9
Secondary accession number(s): C3M4Z9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	February 26, 2008
Last modified:	November 16, 2011
This is version 31 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program