Reviewed, UniProtKB/Swiss-Prot A5F520 (DMA_VIBC3)

Names and origin

Protein names

Recommended name:
    DNA adenine methylase
    EC=2.1.1.72
Alternative name(s):
    M.VchADam
    Deoxyadenosyl-methyltransferase
    DNA adenine methyltransferase

Gene names

Name:	dam
Ordered Locus Names:	VC0395_A2203

Organism

Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) [Complete proteome] [HAMAP]

Taxonomic identifier

345073 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Methylates DNA within the sequence GATC. Directly involved in methyl-directed DNA mismatch repair By similarity.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 277	277	DNA adenine methylase		PRO_0000324810
Sites
	Binding site	10	1	S-adenosyl-L-methionine By similarity
	Binding site	14	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	54	1	S-adenosyl-L-methionine By similarity
	Binding site	181	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5F520-1 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 48D831240F9E7C60	FASTA	277	31,624
	10 20 30 40 50 60 MKKQRAFLKW AGGKYSLVED IQRHLPEARE LVEPFVGAGS VFLNTDFERY LLADINPDLI 70 80 90 100 110 120 NFYNLLKTEP QAYIHEAKRW FVPENNRKEV YLDIRKQFNQ SDDAMFRSLA FLYMNRFGFN 130 140 150 160 170 180 GLCRYNKKGG FNVPFGSYKK PYFPEQELEF FAEKAQRATF ICASYGETFA RAQSDSVIYC 190 200 210 220 230 240 DPPYAPLSTT ANFTSYAGNG FTLDDQAALA DIAEKTAKER GISVLISNHD TTHTRRLYRG 250 260 270 AQLNVVKANR TISRNGAGRN KVDELLALFT PHLSSQA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA adenine methylase is essential for viability and plays a role in the pathogenesis of Yersinia pseudotuberculosis and Vibrio cholerae." Julio S.M., Heithoff D.M., Provenzano D., Klose K.E., Sinsheimer R.L., Low D.A., Mahan M.J. Infect. Immun. 69:7610-7615(2001) [PubMed: 11705940] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Heidelberg J. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF274317 Genomic DNA. Translation: AAG23174.1. CP000627 Genomic DNA. Translation: ABQ21457.1.
3D structure databases
HSSP	HSSP built from PDB template 2DPM based on UniProtKB P04043.
ModBase	Search...
Protein-protein interaction databases
STRING	A5F520.
Protein family/group databases
REBASE	5786. M.VchO395Dam.
Genome annotation databases
GenomeReviews	Gene locus VC0395_A2203 in contig CP000627_GR.
KEGG	vco:VC0395_A2203.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	TSAFTSY.
Family and domain databases
InterPro	IPR002294. D12N6_MeTrfase. IPR012326. Dam. IPR002052. DNA_methylase_N6_adenine_CS. IPR012327. MeTrfase_D12. [Graphical view]
Pfam	PF02086. MethyltransfD12. 1 hit. [Graphical view]
PRINTS	PR00505. D12N6MTFRASE.
TIGRFAMs	TIGR00571. dam. 1 hit.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DMA_VIBC3

Accession

Primary (citable) accession number: A5F520
Secondary accession number(s): Q08318, Q9KNV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	June 12, 2007
Last modified:	November 3, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)