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A5F520

- DMA_VIBC3

UniProt

A5F520 - DMA_VIBC3

Protein

DNA adenine methylase

Gene

dam

Organism
Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 55 (01 Oct 2014)
      Sequence version 1 (12 Jun 2007)
      Previous versions | rss
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    Functioni

    Methylates DNA within the sequence GATC. Directly involved in methyl-directed DNA mismatch repair By similarity.By similarity

    Catalytic activityi

    S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei10 – 101S-adenosyl-L-methionineBy similarity
    Binding sitei14 – 141S-adenosyl-L-methionine; via amide nitrogenBy similarity
    Binding sitei54 – 541S-adenosyl-L-methionineBy similarity
    Binding sitei181 – 1811S-adenosyl-L-methionineBy similarity

    GO - Molecular functioni

    1. nucleic acid binding Source: InterPro
    2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi15105. M.VchO395ADamP.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA adenine methylase (EC:2.1.1.72)
    Alternative name(s):
    DNA adenine methyltransferase
    Deoxyadenosyl-methyltransferase
    M.VchADam
    Gene namesi
    Name:dam
    Ordered Locus Names:VC0395_A2203, VC395_2739
    OrganismiVibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)
    Taxonomic identifieri345073 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
    ProteomesiUP000000249: Chromosome 2, UP000001630: Chromosome I

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 277277DNA adenine methylasePRO_0000324810Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi345073.VC0395_A2203.

    Structurei

    3D structure databases

    ProteinModelPortaliA5F520.
    SMRiA5F520. Positions 3-271.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Phylogenomic databases

    eggNOGiCOG0338.
    HOGENOMiHOG000281348.
    KOiK06223.
    OMAiKFTREIY.

    Family and domain databases

    Gene3Di1.10.1020.10. 1 hit.
    3.40.50.150. 2 hits.
    InterProiIPR023095. Ade_MeTrfase_dom_2.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR012263. M_m6A_EcoRV.
    IPR012327. MeTrfase_D12.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF02086. MethyltransfD12. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
    PRINTSiPR00505. D12N6MTFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00571. dam. 1 hit.
    PROSITEiPS00092. N6_MTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A5F520-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKKQRAFLKW AGGKYSLVED IQRHLPEARE LVEPFVGAGS VFLNTDFERY    50
    LLADINPDLI NFYNLLKTEP QAYIHEAKRW FVPENNRKEV YLDIRKQFNQ 100
    SDDAMFRSLA FLYMNRFGFN GLCRYNKKGG FNVPFGSYKK PYFPEQELEF 150
    FAEKAQRATF ICASYGETFA RAQSDSVIYC DPPYAPLSTT ANFTSYAGNG 200
    FTLDDQAALA DIAEKTAKER GISVLISNHD TTHTRRLYRG AQLNVVKANR 250
    TISRNGAGRN KVDELLALFT PHLSSQA 277
    Length:277
    Mass (Da):31,624
    Last modified:June 12, 2007 - v1
    Checksum:i48D831240F9E7C60
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF274317 Genomic DNA. Translation: AAG23174.1.
    CP000627 Genomic DNA. Translation: ABQ21457.1.
    CP001235 Genomic DNA. Translation: ACP10724.1.
    RefSeqiWP_000744680.1. NC_012582.1.
    YP_001218119.1. NC_009457.1.
    YP_002820960.1. NC_012582.1.

    Genome annotation databases

    EnsemblBacteriaiABQ21457; ABQ21457; VC0395_A2203.
    ACP10724; ACP10724; VC395_2739.
    GeneIDi5136322.
    7776466.
    KEGGivco:VC0395_A2203.
    vcr:VC395_2739.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF274317 Genomic DNA. Translation: AAG23174.1 .
    CP000627 Genomic DNA. Translation: ABQ21457.1 .
    CP001235 Genomic DNA. Translation: ACP10724.1 .
    RefSeqi WP_000744680.1. NC_012582.1.
    YP_001218119.1. NC_009457.1.
    YP_002820960.1. NC_012582.1.

    3D structure databases

    ProteinModelPortali A5F520.
    SMRi A5F520. Positions 3-271.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 345073.VC0395_A2203.

    Protein family/group databases

    REBASEi 15105. M.VchO395ADamP.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABQ21457 ; ABQ21457 ; VC0395_A2203 .
    ACP10724 ; ACP10724 ; VC395_2739 .
    GeneIDi 5136322.
    7776466.
    KEGGi vco:VC0395_A2203.
    vcr:VC395_2739.

    Phylogenomic databases

    eggNOGi COG0338.
    HOGENOMi HOG000281348.
    KOi K06223.
    OMAi KFTREIY.

    Family and domain databases

    Gene3Di 1.10.1020.10. 1 hit.
    3.40.50.150. 2 hits.
    InterProi IPR023095. Ade_MeTrfase_dom_2.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR012263. M_m6A_EcoRV.
    IPR012327. MeTrfase_D12.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF02086. MethyltransfD12. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000398. M_m6A_EcoRV. 1 hit.
    PRINTSi PR00505. D12N6MTFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00571. dam. 1 hit.
    PROSITEi PS00092. N6_MTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "DNA adenine methylase is essential for viability and plays a role in the pathogenesis of Yersinia pseudotuberculosis and Vibrio cholerae."
      Julio S.M., Heithoff D.M., Provenzano D., Klose K.E., Sinsheimer R.L., Low D.A., Mahan M.J.
      Infect. Immun. 69:7610-7615(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Heidelberg J.
      Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 39541 / Classical Ogawa 395 / O395.
    3. "A recalibrated molecular clock and independent origins for the cholera pandemic clones."
      Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J., Wang W., Wang J., Qian W., Li D., Wang L.
      PLoS ONE 3:E4053-E4053(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 39541 / Classical Ogawa 395 / O395.

    Entry informationi

    Entry nameiDMA_VIBC3
    AccessioniPrimary (citable) accession number: A5F520
    Secondary accession number(s): C3LXM4, Q08318, Q9KNV4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: June 12, 2007
    Last modified: October 1, 2014
    This is version 55 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3