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A5F520

- DMA_VIBC3

UniProt

A5F520 - DMA_VIBC3

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Protein

DNA adenine methylase

Gene

dam

Organism
Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Methylates DNA within the sequence GATC. Directly involved in methyl-directed DNA mismatch repair (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101S-adenosyl-L-methionineBy similarity
Binding sitei14 – 141S-adenosyl-L-methionine; via amide nitrogenBy similarity
Binding sitei54 – 541S-adenosyl-L-methionineBy similarity
Binding sitei181 – 1811S-adenosyl-L-methionineBy similarity

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi15105. M.VchO395ADamP.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA adenine methylase (EC:2.1.1.72)
Alternative name(s):
DNA adenine methyltransferase
Deoxyadenosyl-methyltransferase
M.VchADam
Gene namesi
Name:dam
Ordered Locus Names:VC0395_A2203, VC395_2739
OrganismiVibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)
Taxonomic identifieri345073 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000000249: Chromosome 2, UP000001630: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 277277DNA adenine methylasePRO_0000324810Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi345073.VC0395_A2203.

Structurei

3D structure databases

ProteinModelPortaliA5F520.
SMRiA5F520. Positions 3-271.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Phylogenomic databases

eggNOGiCOG0338.
HOGENOMiHOG000281348.
KOiK06223.
OMAiKFTREIY.

Family and domain databases

Gene3Di1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProiIPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PIRSFiPIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00571. dam. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5F520-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKQRAFLKW AGGKYSLVED IQRHLPEARE LVEPFVGAGS VFLNTDFERY
60 70 80 90 100
LLADINPDLI NFYNLLKTEP QAYIHEAKRW FVPENNRKEV YLDIRKQFNQ
110 120 130 140 150
SDDAMFRSLA FLYMNRFGFN GLCRYNKKGG FNVPFGSYKK PYFPEQELEF
160 170 180 190 200
FAEKAQRATF ICASYGETFA RAQSDSVIYC DPPYAPLSTT ANFTSYAGNG
210 220 230 240 250
FTLDDQAALA DIAEKTAKER GISVLISNHD TTHTRRLYRG AQLNVVKANR
260 270
TISRNGAGRN KVDELLALFT PHLSSQA
Length:277
Mass (Da):31,624
Last modified:June 12, 2007 - v1
Checksum:i48D831240F9E7C60
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF274317 Genomic DNA. Translation: AAG23174.1.
CP000627 Genomic DNA. Translation: ABQ21457.1.
CP001235 Genomic DNA. Translation: ACP10724.1.
RefSeqiWP_000744680.1. NC_012582.1.
YP_001218119.1. NC_009457.1.
YP_002820960.1. NC_012582.1.

Genome annotation databases

EnsemblBacteriaiABQ21457; ABQ21457; VC0395_A2203.
ACP10724; ACP10724; VC395_2739.
GeneIDi5136322.
7776466.
KEGGivco:VC0395_A2203.
vcr:VC395_2739.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF274317 Genomic DNA. Translation: AAG23174.1 .
CP000627 Genomic DNA. Translation: ABQ21457.1 .
CP001235 Genomic DNA. Translation: ACP10724.1 .
RefSeqi WP_000744680.1. NC_012582.1.
YP_001218119.1. NC_009457.1.
YP_002820960.1. NC_012582.1.

3D structure databases

ProteinModelPortali A5F520.
SMRi A5F520. Positions 3-271.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 345073.VC0395_A2203.

Protein family/group databases

REBASEi 15105. M.VchO395ADamP.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABQ21457 ; ABQ21457 ; VC0395_A2203 .
ACP10724 ; ACP10724 ; VC395_2739 .
GeneIDi 5136322.
7776466.
KEGGi vco:VC0395_A2203.
vcr:VC395_2739.

Phylogenomic databases

eggNOGi COG0338.
HOGENOMi HOG000281348.
KOi K06223.
OMAi KFTREIY.

Family and domain databases

Gene3Di 1.10.1020.10. 1 hit.
3.40.50.150. 2 hits.
InterProi IPR023095. Ade_MeTrfase_dom_2.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR012263. M_m6A_EcoRV.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF02086. MethyltransfD12. 1 hit.
[Graphical view ]
PIRSFi PIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTSi PR00505. D12N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00571. dam. 1 hit.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "DNA adenine methylase is essential for viability and plays a role in the pathogenesis of Yersinia pseudotuberculosis and Vibrio cholerae."
    Julio S.M., Heithoff D.M., Provenzano D., Klose K.E., Sinsheimer R.L., Low D.A., Mahan M.J.
    Infect. Immun. 69:7610-7615(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Heidelberg J.
    Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39541 / Classical Ogawa 395 / O395.
  3. "A recalibrated molecular clock and independent origins for the cholera pandemic clones."
    Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J., Wang W., Wang J., Qian W., Li D., Wang L.
    PLoS ONE 3:E4053-E4053(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39541 / Classical Ogawa 395 / O395.

Entry informationi

Entry nameiDMA_VIBC3
AccessioniPrimary (citable) accession number: A5F520
Secondary accession number(s): C3LXM4, Q08318, Q9KNV4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: June 12, 2007
Last modified: October 1, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3