A5F520 (DMA_VIBC3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA adenine methylase
EC=2.1.1.72

Alternative name(s):
DNA adenine methyltransferase
Deoxyadenosyl-methyltransferase
M.VchADam

Gene names

Name:	dam
Ordered Locus Names:	VC0395_A2203, VC395_2739

Organism

Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) [Complete proteome] [HAMAP]

Taxonomic identifier

345073 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Methylates DNA within the sequence GATC. Directly involved in methyl-directed DNA mismatch repair By similarity.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 277	277	DNA adenine methylase		PRO_0000324810
Sites
	Binding site	10	1	S-adenosyl-L-methionine By similarity
	Binding site	14	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	54	1	S-adenosyl-L-methionine By similarity
	Binding site	181	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5F520 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 48D831240F9E7C60	FASTA	277	31,624
	10 20 30 40 50 60 MKKQRAFLKW AGGKYSLVED IQRHLPEARE LVEPFVGAGS VFLNTDFERY LLADINPDLI 70 80 90 100 110 120 NFYNLLKTEP QAYIHEAKRW FVPENNRKEV YLDIRKQFNQ SDDAMFRSLA FLYMNRFGFN 130 140 150 160 170 180 GLCRYNKKGG FNVPFGSYKK PYFPEQELEF FAEKAQRATF ICASYGETFA RAQSDSVIYC 190 200 210 220 230 240 DPPYAPLSTT ANFTSYAGNG FTLDDQAALA DIAEKTAKER GISVLISNHD TTHTRRLYRG 250 260 270 AQLNVVKANR TISRNGAGRN KVDELLALFT PHLSSQA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA adenine methylase is essential for viability and plays a role in the pathogenesis of Yersinia pseudotuberculosis and Vibrio cholerae." Julio S.M., Heithoff D.M., Provenzano D., Klose K.E., Sinsheimer R.L., Low D.A., Mahan M.J. Infect. Immun. 69:7610-7615(2001) [PubMed: 11705940] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Heidelberg J. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39541 / Ogawa 395 / O395.
[3]	"A recalibrated molecular clock and independent origins for the cholera pandemic clones." Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J., Wang W., Wang J., Qian W., Li D., Wang L. PLoS ONE 3:E4053-E4053(2008) [PubMed: 19115014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39541 / Ogawa 395 / O395.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF274317 Genomic DNA. Translation: AAG23174.1. CP000627 Genomic DNA. Translation: ABQ21457.1. CP001235 Genomic DNA. Translation: ACP10724.1.
3D structure databases
ProteinModelPortal	A5F520.
SMR	A5F520. Positions 3-271.
ModBase	Search...
Protein-protein interaction databases
STRING	A5F520.
Protein family/group databases
REBASE	15105. M.VchO395ADamP. 20804. M.VchO395CDamP. 5786. M.VchO395Dam.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GenomeReviews	Gene locus VC0395_A2203 in contig CP000627_GR. Gene locus VC395_2739 in contig CP001235_GR.
KEGG	vco:VC0395_A2203.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0338.
HOGENOM	HBG586381.
OMA	AFEQTRS.
ProtClustDB	CLSK874863.
Enzyme and pathway databases
BioCyc	VCHO345073:VC0395_A2203-MONOMER.
Family and domain databases
InterPro	IPR023095. Ade_MeTrfase_dom_2. IPR002052. DNA_methylase_N6_adenine_CS. IPR012263. M_m6A_EcoRV. IPR012327. MeTrfase_D12. [Graphical view]
Gene3D	G3DSA:1.10.1020.10. Ade_MeTrfase_dom_2. 1 hit.
KO	K06223.
Pfam	PF02086. MethyltransfD12. 1 hit. [Graphical view]
PIRSF	PIRSF000398. M_m6A_EcoRV. 1 hit.
PRINTS	PR00505. D12N6MTFRASE.
TIGRFAMs	TIGR00571. Dam. 1 hit.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DMA_VIBC3

Accession

Primary (citable) accession number: A5F520
Secondary accession number(s): C3LXM4, Q08318, Q9KNV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	June 12, 2007
Last modified:	November 16, 2011
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program