A5F4K5 (STHA_VIBC3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Soluble pyridine nucleotide transhydrogenase
Short name=STH
EC=1.6.1.1

Alternative name(s):
NAD(P)(+) transhydrogenase [B-specific]

Gene names

Name:	sthA
Synonyms:	dude
Ordered Locus Names:	VC0395_A2370, VC395_0029

Organism

Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) [Complete proteome] [HAMAP]

Taxonomic identifier

345073 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Conversion of NADPH, generated by peripheral catabolic pathways, to NADH, which can enter the respiratory chain for energy generation By similarity. HAMAP MF_00247
Catalytic activity	NADPH + NAD+ = NADP+ + NADH. HAMAP MF_00247
Cofactor	Binds 1 FAD per subunit By similarity. HAMAP MF_00247
Subcellular location	Cytoplasm By similarity HAMAP MF_00247.
Sequence similarities	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD(P)+ transhydrogenase (B-specific) activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 466	466	Soluble pyridine nucleotide transhydrogenase HAMAP MF_00247		PRO_1000071844
Regions
	Nucleotide binding	36 – 45	10	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5F4K5 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 60D05DB3FD595BE1	FASTA	466	50,942
	10 20 30 40 50 60 MGQKNHFDVI VIGSGPGGEG AAMGLTKGGK NVAIIEKESS VGGGCTHWGT IPSKALRHAV 70 80 90 100 110 120 SRIIEFNSNP LFCKNNSSIH ATFSTILSHA KSVIDKQTRL RQGFYDRNQC TLIFGAAHFI 130 140 150 160 170 180 DAHTVAVKKA DGSIDTYSAD KFVIATGSRP YHPKDVDFGH PRIYDSDSIL NLEHDPRHII 190 200 210 220 230 240 IYGAGVIGCE YASIFRGLDV KTDLINTRDR LLSFLDNEVS DALSYHFWNS GVVIRNDETY 250 260 270 280 290 300 DKVEGTSDGV IVHLKSGKKM RADCLLYANG RTGNTDKLNL ESVGLQADSR GQLVVNANYQ 310 320 330 340 350 360 TQVEHIYAVG DVIGYPSLAS AAYDQGRFVA QAIIHGQAAH LLTEDIPTGI YTIPEISSVG 370 380 390 400 410 420 RTEQELTAAK VPYEVGRASF KHLARAQIAG KDIGSLKILF HRETKEILGI HCFGERAAEI 430 440 450 460 IHIGQAIMEQ KGEANTIEYF VNTTFNYPTM AEAFRVAALN GLNRLF

References

[1]	Heidelberg J. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39541 / Ogawa 395 / O395.
[2]	"A recalibrated molecular clock and independent origins for the cholera pandemic clones." Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J., Wang W., Wang J., Qian W., Li D., Wang L. PLoS ONE 3:E4053-E4053(2008) [PubMed: 19115014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39541 / Ogawa 395 / O395.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000627 Genomic DNA. Translation: ABQ21088.1. CP001235 Genomic DNA. Translation: ACP08058.1.
3D structure databases
ProteinModelPortal	A5F4K5.
ModBase	Search...
Protein-protein interaction databases
STRING	A5F4K5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GenomeReviews	Gene locus VC0395_A2370 in contig CP000627_GR. Gene locus VC395_0029 in contig CP001235_GR.
KEGG	vco:VC0395_A2370.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1249.
HOGENOM	HBG515043.
OMA	GEGNTIE.
ProtClustDB	PRK05249.
Enzyme and pathway databases
BioCyc	VCHO345073:VC0395_A2370-MONOMER.
Family and domain databases
HAMAP	MF_00247. SthA. [Tree]
InterPro	IPR016156. FAD/NAD-linked_Rdtase_dimer. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD. IPR001327. Pyr_OxRdtase_NAD-bd_dom. IPR022962. STH. [Graphical view]
Gene3D	G3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
KO	K00322.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
SUPFAM	SSF55424. FAD/NAD-linked_reductase_dimer. 1 hit.
ProtoNet	Search...

Entry information

Entry name

STHA_VIBC3

Accession

Primary (citable) accession number: A5F4K5
Secondary accession number(s): C3M294

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	June 12, 2007
Last modified:	November 16, 2011
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program