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Protein

3-ketoacyl-CoA thiolase

Gene

fadA

Organism
Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed.UniRule annotation

Catalytic activityi

Acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA.UniRule annotation

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei91Acyl-thioester intermediateUniRule annotation1
Active sitei343Proton acceptorUniRule annotation1
Active sitei373Proton acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processFatty acid metabolism, Lipid degradation, Lipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
3-ketoacyl-CoA thiolaseUniRule annotation (EC:2.3.1.16UniRule annotation)
Alternative name(s):
Acetyl-CoA acyltransferaseUniRule annotation
Beta-ketothiolaseUniRule annotation
Fatty acid oxidation complex subunit betaUniRule annotation
Gene namesi
Name:fadAUniRule annotation
Ordered Locus Names:VC0395_A2533, VC395_0196
OrganismiVibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)
Taxonomic identifieri345073 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000249 Componenti: Chromosome 2

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003235561 – 3873-ketoacyl-CoA thiolaseAdd BLAST387

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadB) and two beta chains (FadA).UniRule annotation

Protein-protein interaction databases

STRINGi345073.VC0395_A2533.

Structurei

3D structure databases

ProteinModelPortaliA5F464.
SMRiA5F464.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thiolase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CHU. Bacteria.
COG0183. LUCA.
HOGENOMiHOG000012239.
KOiK00632.
OMAiCGGTESM.
OrthoDBiPOG091H0I7B.

Family and domain databases

CDDicd00751. thiolase. 1 hit.
Gene3Di3.40.47.10. 2 hits.
HAMAPiMF_01620. FadA. 1 hit.
InterProiView protein in InterPro
IPR012805. FadA.
IPR002155. Thiolase.
IPR016039. Thiolase-like.
IPR020615. Thiolase_acyl_enz_int_AS.
IPR020610. Thiolase_AS.
IPR020617. Thiolase_C.
IPR020613. Thiolase_CS.
IPR020616. Thiolase_N.
PfamiView protein in Pfam
PF02803. Thiolase_C. 1 hit.
PF00108. Thiolase_N. 1 hit.
PIRSFiPIRSF000429. Ac-CoA_Ac_transf. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
TIGRFAMsiTIGR01930. AcCoA-C-Actrans. 1 hit.
TIGR02445. fadA. 1 hit.
PROSITEiView protein in PROSITE
PS00098. THIOLASE_1. 1 hit.
PS00737. THIOLASE_2. 1 hit.
PS00099. THIOLASE_3. 1 hit.

Sequencei

Sequence statusi: Complete.

A5F464-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTVVIVDCL RTPMGRSKGG AFRHQRAEDL SAHLMKGILA RNPQVNPKEI
60 70 80 90 100
EDIYWGCVQQ TLEQGFNVAR NAALLAGLPI EIGAVTVNRL CGSSMQALHD
110 120 130 140 150
AARAIMVGDA EICLVGGVEH MGHVPMTHGV DFHPGLSKNV AKAAGMMGLT
160 170 180 190 200
AEMLGKLHGI SRQQQDEFAA RSHARAHAAT LEGRFKNEIL PTEGHAADGT
210 220 230 240 250
LFTLDYDEVI RPETTVAGLA ELRPVFDPAN GTVTAGTSSA LSDGASAMLV
260 270 280 290 300
MSEQKAKALG LTIRARIKAM AVAGCDPSIM GYGPVPATHK ALKRAGLTMQ
310 320 330 340 350
DMDVVELNEA FAAQSLPCAK DLGLLDMMDD KVNLNGGAIA LGHPLGCSGT
360 370 380
RISTTLINLM EAKDAKYGLA TMCIGLGQGI ATIFERP
Length:387
Mass (Da):40,828
Last modified:June 12, 2007 - v1
Checksum:iA02AD9651DDC94CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000627 Genomic DNA. Translation: ABQ20531.1.
CP001235 Genomic DNA. Translation: ACP08223.1.
RefSeqiWP_001099261.1. NC_012582.1.

Genome annotation databases

EnsemblBacteriaiABQ20531; ABQ20531; VC0395_A2533.
ACP08223; ACP08223; VC395_0196.
KEGGivco:VC0395_A2533.
vcr:VC395_0196.
PATRICifig|345073.21.peg.185.

Similar proteinsi

Entry informationi

Entry nameiFADA_VIBC3
AccessioniPrimary (citable) accession number: A5F464
Secondary accession number(s): C3M344
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: June 12, 2007
Last modified: August 30, 2017
This is version 71 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families