Reviewed, UniProtKB/Swiss-Prot A5F3I6 (CYSH_VIBC3)

Names and origin

Protein names

Recommended name:
    Phosphoadenosine phosphosulfate reductase
    EC=1.8.4.8
Alternative name(s):
    PAPS reductase, thioredoxin dependent
    PAdoPS reductase
    3'-phosphoadenylylsulfate reductase
    PAPS sulfotransferase

Gene names

Name:	cysH
Ordered Locus Names:	VC0395_A2797

Organism

Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) [Complete proteome] [HAMAP]

Taxonomic identifier

345073 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduction of activated sulfate into sulfite.
Catalytic activity	Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin.
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 3/3.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the PAPS reductase family. CysH subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	phosphoadenylyl-sulfate reductase (thioredoxin) activity Inferred from electronic annotation. Source: HAMAP transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 253	253	Phosphoadenosine phosphosulfate reductase		PRO_1000071166

Sequences

	Sequence		Length	Mass (Da)
	A5F3I6-1 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: A92EF4BEE08A9FFD	FASTA	253	29,249
	10 20 30 40 50 60 MPNRTVPTLE ELLTLNKVQQ TLRLTEVNQH LESLTAQERV VWGLENLQGN HALSSSFGIQ 70 80 90 100 110 120 AAVMLHLLTS VKSDIPVVLT DTGYLFPETY QFIDELTERL NLNLKVYSAP VSAAWQEARY 130 140 150 160 170 180 GKLWEQGVEG IERYNQINKV EPMRRALDEL NIGTWFSGLR REQSQSRASL PILSVQNGVF 190 200 210 220 230 240 KFLPVIDWTN KEVHYYLKDN DLPYHPLWEQ GYLSVGDTHT TQKWQPGMNE EQTRFFGLKR 250 ECGLHEDHND THQ

References

[1]	Heidelberg J. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000627 Genomic DNA. Translation: ABQ21825.1.
RefSeq	YP_001218655.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5136488.
GenomeReviews	Gene locus VC0395_A2797 in contig CP000627_GR.
KEGG	vco:VC0395_A2797.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00063. [Tree]
InterPro	IPR004511. CysH. IPR002500. PAPS_reduct. IPR011800. PAPS_reductase. [Graphical view]
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
TIGRFAMs	TIGR00434. cysH. 1 hit. TIGR02057. PAPS_reductase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSH_VIBC3

Accession

Primary (citable) accession number: A5F3I6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	June 12, 2007
Last modified:	November 25, 2008
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)