A5F3I6 (CYSH_VIBC3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoadenosine phosphosulfate reductase
EC=1.8.4.8

Alternative name(s):
3'-phosphoadenylylsulfate reductase
PAPS reductase, thioredoxin dependent
PAPS sulfotransferase
PAdoPS reductase

Gene names

Name:	cysH
Ordered Locus Names:	VC0395_A2797, VC395_0430

Organism

Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) [Complete proteome] [HAMAP]

Taxonomic identifier

345073 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Reduction of activated sulfate into sulfite. HAMAP MF_00063
Catalytic activity	Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin. HAMAP MF_00063
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 3/3. HAMAP MF_00063
Subcellular location	Cytoplasm By similarity HAMAP MF_00063.
Sequence similarities	Belongs to the PAPS reductase family. CysH subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: InterPro sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphoadenylyl-sulfate reductase (thioredoxin) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 253	253	Phosphoadenosine phosphosulfate reductase HAMAP MF_00063		PRO_1000071166

Sequences

	Sequence		Length	Mass (Da)
	A5F3I6 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: A92EF4BEE08A9FFD	FASTA	253	29,249
	10 20 30 40 50 60 MPNRTVPTLE ELLTLNKVQQ TLRLTEVNQH LESLTAQERV VWGLENLQGN HALSSSFGIQ 70 80 90 100 110 120 AAVMLHLLTS VKSDIPVVLT DTGYLFPETY QFIDELTERL NLNLKVYSAP VSAAWQEARY 130 140 150 160 170 180 GKLWEQGVEG IERYNQINKV EPMRRALDEL NIGTWFSGLR REQSQSRASL PILSVQNGVF 190 200 210 220 230 240 KFLPVIDWTN KEVHYYLKDN DLPYHPLWEQ GYLSVGDTHT TQKWQPGMNE EQTRFFGLKR 250 ECGLHEDHND THQ

References

[1]	Heidelberg J. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39541 / Ogawa 395 / O395.
[2]	"A recalibrated molecular clock and independent origins for the cholera pandemic clones." Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J., Wang W., Wang J., Qian W., Li D., Wang L. PLoS ONE 3:E4053-E4053(2008) [PubMed: 19115014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39541 / Ogawa 395 / O395.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000627 Genomic DNA. Translation: ABQ21825.1. CP001235 Genomic DNA. Translation: ACP08452.1.
3D structure databases
ProteinModelPortal	A5F3I6.
SMR	A5F3I6. Positions 9-246.
ModBase	Search...
Protein-protein interaction databases
STRING	A5F3I6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GenomeReviews	Gene locus VC0395_A2797 in contig CP000627_GR. Gene locus VC395_0430 in contig CP001235_GR.
KEGG	vco:VC0395_A2797.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0175.
HOGENOM	HBG758022.
OMA	AIHGTRF.
ProtClustDB	PRK02090.
Enzyme and pathway databases
BioCyc	VCHO345073:VC0395_A2797-MONOMER.
Family and domain databases
HAMAP	MF_00063. CysH. [Tree]
InterPro	IPR004511. PAPS/APS_Rdtase. IPR002500. PAPS_reduct. IPR011800. PAPS_reductase_CysH. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K00390.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
TIGRFAMs	TIGR00434. CysH. 1 hit. TIGR02057. PAPS_reductase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSH_VIBC3

Accession

Primary (citable) accession number: A5F3I6
Secondary accession number(s): C3M4I0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	June 12, 2007
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program