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A5F2X0

- SYL_VIBC3

UniProt

A5F2X0 - SYL_VIBC3

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Protein

Leucine--tRNA ligase

Gene
leuS, VC0395_A0478, VC395_0971
Organism
Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei621 – 6211ATP By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligase (EC:6.1.1.4)
Alternative name(s):
Leucyl-tRNA synthetase
Short name:
LeuRS
Gene namesi
Name:leuS
Ordered Locus Names:VC0395_A0478, VC395_0971
OrganismiVibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)
Taxonomic identifieri345073 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000000249: Chromosome 2, UP000001630: Chromosome I

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 858858Leucine--tRNA ligaseUniRule annotationPRO_0000334836Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi345073.VC0395_A0478.

Structurei

3D structure databases

ProteinModelPortaliA5F2X0.
SMRiA5F2X0. Positions 228-413.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionUniRule annotationAdd
BLAST
Motifi618 – 6225"KMSKS" regionUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5F2X0-1 [UniParc]FASTAAdd to Basket

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MQEQYNPQDI EHKVQQHWDN NKTFVVSEDP NKEKFYCLSM FPYPSGRLHM    50
GHVRNYTIGD VVSRFQRLQG KNVLQPIGWD AFGLPAENAA VKNNTAPAPW 100
TYENIEYMKN QLKLLGFGYD WNREFATCRP EYYRWEQEFF TKLFAKGLVY 150
KKTSSVNWCP NDQTVLANEQ VEDGCCWRCD TPVEQKEIPQ WFIKITAYAQ 200
ELLDDLDNLD GWPEMVKTMQ RNWIGRSEGV ELKFAVKGEN TDLEVYTTRP 250
DTLMGVTYVG IAAGHPLATK AAANNPALAA FIDECKNTKV AEAEIATMEK 300
KGMATGLTAI HPLNGREVPI YIANFVLMDY GTGAVMAVPA HDQRDFEFAT 350
KYGLDIIPVI KPADGSELDV SEAAYTEKGV LFASGEFDGL DFQAAFNAIA 400
AKLEAEGKGK KTVNFRLRDW GVSRQRYWGA PIPMVTTEDG QVHPVPADQL 450
PVILPEDVVM DGVTSPIKAD KEWAKTTFNG EPALRETDTF DTFMESSWYY 500
ARYCSPQADD ILDPEKANYW LPVDQYIGGI EHACMHLLYS RFFYKLLRDA 550
GYVKSDEPFK KLLCQGMVLA DAFYYTNDKG GKEWVSPTEV KVERDGKGRI 600
VSAVDATGRQ VEHSGMIKMS KSKNNGIDPQ EMVDKYGADT VRLFMMFASP 650
ADMTLEWQES GVEGANRFLR RVWKLVREHT ELGQAPALDA SALNADQKAL 700
RRDVHKTIAK VTDDVARRQT FNTAIAAIME LMNKLTKAPM TEAQDRAILD 750
EALKAITLML YPITPHICFE MWVALGQSNI DTASWPTYDE AALVEDEKLI 800
VLQVNGKLRG KLTVAADATQ QQVEALGMQD ENVQKFIDGL TVRKVIYVPG 850
KLLNIVAN 858
Length:858
Mass (Da):96,647
Last modified:May 20, 2008 - v2
Checksum:i51DA349A099B083C
GO

Sequence cautioni

The sequence ABQ20041.1 differs from that shown. Reason: Erroneous initiation.
The sequence ACP08983.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000627 Genomic DNA. Translation: ABQ20041.1. Different initiation.
CP001235 Genomic DNA. Translation: ACP08983.1. Different initiation.
RefSeqiYP_001216429.1. NC_009457.1.
YP_002819219.1. NC_012582.1.

Genome annotation databases

EnsemblBacteriaiABQ20041; ABQ20041; VC0395_A0478.
ACP08983; ACP08983; VC395_0971.
GeneIDi5137275.
7774953.
KEGGivco:VC0395_A0478.
vcr:VC395_0971.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000627 Genomic DNA. Translation: ABQ20041.1 . Different initiation.
CP001235 Genomic DNA. Translation: ACP08983.1 . Different initiation.
RefSeqi YP_001216429.1. NC_009457.1.
YP_002819219.1. NC_012582.1.

3D structure databases

ProteinModelPortali A5F2X0.
SMRi A5F2X0. Positions 228-413.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 345073.VC0395_A0478.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABQ20041 ; ABQ20041 ; VC0395_A0478 .
ACP08983 ; ACP08983 ; VC395_0971 .
GeneIDi 5137275.
7774953.
KEGGi vco:VC0395_A0478.
vcr:VC395_0971.

Phylogenomic databases

eggNOGi COG0495.
HOGENOMi HOG000200747.
KOi K01869.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPi MF_00049_B. Leu_tRNA_synth_B.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF7. PTHR11946:SF7. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view ]
PRINTSi PR00985. TRNASYNTHLEU.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Heidelberg J.
    Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39541 / Classical Ogawa 395 / O395.
  2. "A recalibrated molecular clock and independent origins for the cholera pandemic clones."
    Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J., Wang W., Wang J., Qian W., Li D., Wang L.
    PLoS ONE 3:E4053-E4053(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39541 / Classical Ogawa 395 / O395.

Entry informationi

Entry nameiSYL_VIBC3
AccessioniPrimary (citable) accession number: A5F2X0
Secondary accession number(s): C3LYW7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: May 14, 2014
This is version 52 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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