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Protein

Glutamine--tRNA ligase

Gene

glnS

Organism
Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei271 – 2711ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. glutaminyl-tRNA aminoacylation Source: InterPro
  2. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--tRNA ligaseUniRule annotation (EC:6.1.1.18UniRule annotation)
Alternative name(s):
Glutaminyl-tRNA synthetaseUniRule annotation
Short name:
GlnRSUniRule annotation
Gene namesi
Name:glnSUniRule annotation
Ordered Locus Names:VC0395_A0517, VC395_1012
OrganismiVibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)
Taxonomic identifieri345073 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000000249 Componenti: Chromosome 2 UP000001630 Componenti: Chromosome I

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 556556Glutamine--tRNA ligasePRO_1000071399Add
BLAST

Proteomic databases

PRIDEiA5F2T1.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi345073.VC0395_A0517.

Structurei

3D structure databases

ProteinModelPortaliA5F2T1.
SMRiA5F2T1. Positions 9-549.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi34 – 4411"HIGH" regionAdd
BLAST
Motifi268 – 2725"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000259232.
KOiK01886.
OMAiVTHSICT.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
HAMAPiMF_00126. Gln_tRNA_synth.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR022861. Gln_tRNA_ligase_bac.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00440. glnS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5F2T1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEAEARPSN FIRQIIDKDL ADGKHTTVHT RFPPEPNGYL HIGHAKSICL
60 70 80 90 100
NFGIAQDYQG QCNLRFDDTN PEKENLEYVE SIKKDVTWLG FDWSGEVCYS
110 120 130 140 150
SDYFDKLYEY AIELIQKGLA YVDELTPEQI REYRGTLTEP GKHSPYRDRS
160 170 180 190 200
VEENLALFEK MRAGEFAEGQ ACLRAKIDMA SSFIVMRDPV LYRVRFAEHH
210 220 230 240 250
QTGDKWCIYP MYDFTHCISD ALEGITHSIC TLEFQDNRRL YDWVLDNITI
260 270 280 290 300
PCHPRQYEFS RLNLEYTVMS KRKLNQLVTE KLVTGWDDPR MPTISGLRRR
310 320 330 340 350
GFTPSAIREF CKRIGVTKQE NMIEYSALES CIRDDLNENA PRAMAVLDPV
360 370 380 390 400
KLVIENFAAG TVETLTLANH PNKPEMGDRE VPFTRELWIE REDFREEANK
410 420 430 440 450
KYKRLVLGKE VRLRGAYVIK AERIEKDEQG NITTIFCSYD PETLGKNPAD
460 470 480 490 500
GRKVKGVIHW VSAEKGVPAE FRLYERLFTV PNPGAADNFA ETINPESLVK
510 520 530 540 550
VQGYVEPSLV EAKPEFGYQF ERMGYFCADN KDSSPQALVF NRTVGLRDSF

VKIDEE
Length:556
Mass (Da):64,118
Last modified:June 12, 2007 - v1
Checksum:i8108C0366F1870F8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000627 Genomic DNA. Translation: ABQ21385.1.
CP001235 Genomic DNA. Translation: ACP09024.1.
RefSeqiYP_001216468.1. NC_009457.1.
YP_002819260.1. NC_012582.1.

Genome annotation databases

EnsemblBacteriaiABQ21385; ABQ21385; VC0395_A0517.
ACP09024; ACP09024; VC395_1012.
GeneIDi5136259.
7774989.
KEGGivco:VC0395_A0517.
vcr:VC395_1012.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000627 Genomic DNA. Translation: ABQ21385.1.
CP001235 Genomic DNA. Translation: ACP09024.1.
RefSeqiYP_001216468.1. NC_009457.1.
YP_002819260.1. NC_012582.1.

3D structure databases

ProteinModelPortaliA5F2T1.
SMRiA5F2T1. Positions 9-549.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi345073.VC0395_A0517.

Proteomic databases

PRIDEiA5F2T1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ21385; ABQ21385; VC0395_A0517.
ACP09024; ACP09024; VC395_1012.
GeneIDi5136259.
7774989.
KEGGivco:VC0395_A0517.
vcr:VC395_1012.

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000259232.
KOiK01886.
OMAiVTHSICT.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
HAMAPiMF_00126. Gln_tRNA_synth.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR022861. Gln_tRNA_ligase_bac.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00440. glnS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Heidelberg J.
    Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39541 / Classical Ogawa 395 / O395.
  2. "A recalibrated molecular clock and independent origins for the cholera pandemic clones."
    Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J., Wang W., Wang J., Qian W., Li D., Wang L.
    PLoS ONE 3:E4053-E4053(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39541 / Classical Ogawa 395 / O395.

Entry informationi

Entry nameiSYQ_VIBC3
AccessioniPrimary (citable) accession number: A5F2T1
Secondary accession number(s): C3LZ08
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: June 12, 2007
Last modified: January 7, 2015
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.