A5F2T1 (SYQ_VIBC3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamine--tRNA ligase
EC=6.1.1.18

Alternative name(s):
Glutaminyl-tRNA synthetase
Short name=GlnRS

Gene names

Name:	glnS
Ordered Locus Names:	VC0395_A0517, VC395_1012

Organism

Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) [Complete proteome] [HAMAP]

Taxonomic identifier

345073 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio › Vibrio cholerae

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP-Rule MF_00126
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 556	556	Glutamine--tRNA ligase HAMAP-Rule MF_00126		PRO_1000071399
Regions
	Motif	34 – 44	11	"HIGH" region HAMAP-Rule MF_00126
	Motif	268 – 272	5	"KMSKS" region HAMAP-Rule MF_00126
Sites
	Binding site	271	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5F2T1 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 8108C0366F1870F8	FASTA	556	64,118
	10 20 30 40 50 60 MSEAEARPSN FIRQIIDKDL ADGKHTTVHT RFPPEPNGYL HIGHAKSICL NFGIAQDYQG 70 80 90 100 110 120 QCNLRFDDTN PEKENLEYVE SIKKDVTWLG FDWSGEVCYS SDYFDKLYEY AIELIQKGLA 130 140 150 160 170 180 YVDELTPEQI REYRGTLTEP GKHSPYRDRS VEENLALFEK MRAGEFAEGQ ACLRAKIDMA 190 200 210 220 230 240 SSFIVMRDPV LYRVRFAEHH QTGDKWCIYP MYDFTHCISD ALEGITHSIC TLEFQDNRRL 250 260 270 280 290 300 YDWVLDNITI PCHPRQYEFS RLNLEYTVMS KRKLNQLVTE KLVTGWDDPR MPTISGLRRR 310 320 330 340 350 360 GFTPSAIREF CKRIGVTKQE NMIEYSALES CIRDDLNENA PRAMAVLDPV KLVIENFAAG 370 380 390 400 410 420 TVETLTLANH PNKPEMGDRE VPFTRELWIE REDFREEANK KYKRLVLGKE VRLRGAYVIK 430 440 450 460 470 480 AERIEKDEQG NITTIFCSYD PETLGKNPAD GRKVKGVIHW VSAEKGVPAE FRLYERLFTV 490 500 510 520 530 540 PNPGAADNFA ETINPESLVK VQGYVEPSLV EAKPEFGYQF ERMGYFCADN KDSSPQALVF 550 NRTVGLRDSF VKIDEE

References

[1]	Heidelberg J. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39541 / Ogawa 395 / O395.
[2]	"A recalibrated molecular clock and independent origins for the cholera pandemic clones." Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J., Wang W., Wang J., Qian W., Li D., Wang L. PLoS ONE 3:E4053-E4053(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39541 / Ogawa 395 / O395.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000627 Genomic DNA. Translation: ABQ21385.1. CP001235 Genomic DNA. Translation: ACP09024.1.
RefSeq	YP_001216468.1. NC_009457.1. YP_002819260.1. NC_012582.1.
3D structure databases
ProteinModelPortal	A5F2T1.
SMR	A5F2T1. Positions 9-549.
ModBase	Search...
Protein-protein interaction databases
STRING	345073.VC0395_A0517.
Proteomic databases
PRIDE	A5F2T1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABQ21385; ABQ21385; VC0395_A0517. ACP09024; ACP09024; VC395_1012.
GeneID	5136259. 7774989.
KEGG	vco:VC0395_A0517. vcr:VC395_1012.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HOG000259232.
KO	K01886.
OMA	RMPTIAG.
ProtClustDB	PRK05347.
Family and domain databases
Gene3D	1.10.1160.10. 1 hit. 2.40.240.10. 2 hits. 3.40.50.620. 2 hits.
HAMAP	MF_00126. Gln_tRNA_synth.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth_Ib. IPR022861. Gln_tRNA_ligase_bac. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. IPR011035. Ribosomal_L25/Gln-tRNA_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10119. PTHR10119. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF50715. Ribosomal_L25rel. 1 hit.
TIGRFAMs	TIGR00440. glnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYQ_VIBC3

Accession

Primary (citable) accession number: A5F2T1
Secondary accession number(s): C3LZ08

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	June 12, 2007
Last modified:	May 1, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program