ID   MAO1_VIBC3              Reviewed;         562 AA.
AC   A5F1Z0;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   18-MAR-2008, sequence version 2.
DT   16-JUN-2009, entry version 19.
DE   RecName: Full=NAD-dependent malic enzyme;
DE            Short=NAD-ME;
DE            EC=1.1.1.38;
GN   Name=sfcA; OrderedLocusNames=VC0395_A0809;
OS   Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales;
OC   Vibrionaceae; Vibrio.
OX   NCBI_TaxID=345073;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Heidelberg J.;
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: (S)-malate + NAD(+) = pyruvate + CO(2) + NADH.
CC   -!- COFACTOR: Divalent metal cations. Prefers magnesium or manganese
CC       (By similarity).
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SIMILARITY: Belongs to the malic enzymes family.
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DR   EMBL; CP000627; ABQ21323.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; YP_001216759.1; -.
DR   GeneID; 5136256; -.
DR   GenomeReviews; CP000627_GR; VC0395_A0809.
DR   KEGG; vco:VC0395_A0809; -.
DR   GO; GO:0016619; F:malate dehydrogenase (oxaloacetate-decarbox...; IEA:HAMAP.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0051287; F:NAD or NADH binding; IEA:InterPro.
DR   GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_01619; -; 1.
DR   InterPro; IPR015884; Malic_enzyme_CS.
DR   InterPro; IPR012301; Malic_N.
DR   InterPro; IPR012302; Malic_NAD_bd.
DR   InterPro; IPR001891; Malic_OxRdtase.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF00390; malic; 1.
DR   Pfam; PF03949; Malic_M; 1.
DR   PRINTS; PR00072; MALOXRDTASE.
DR   PROSITE; PS00331; MALIC_ENZYMES; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Metal-binding; NAD; Oxidoreductase.
FT   CHAIN         1    562       NAD-dependent malic enzyme.
FT                                /FTId=PRO_0000323541.
FT   ACT_SITE    101    101       Proton donor (By similarity).
FT   ACT_SITE    172    172       Proton acceptor (By similarity).
FT   METAL       243    243       Divalent metal cation (By similarity).
FT   METAL       244    244       Divalent metal cation (By similarity).
FT   METAL       267    267       Divalent metal cation (By similarity).
FT   BINDING     154    154       NAD (By similarity).
FT   BINDING     267    267       NAD (By similarity).
FT   BINDING     415    415       NAD (By similarity).
FT   SITE        267    267       Important for activity (By similarity).
SQ   SEQUENCE   562 AA;  62153 MW;  934D1EEF53832130 CRC64;
     MNNDKRPLYI SYAGPALLST PLLNKGSAFS AEERASFNLE GLLPEATETI QEQVVRAYQQ
     YRGFESDMDK HIYLRNIQDT NETLFYRLVQ NHISEMMPII YTPTVGAACE NFSNIYRRGR
     GLFISYANRD RIDDLLNNAA NHNVKVIVVT DGERILGLGD QGIGGMGIPI GKLSLYTACG
     GISPAYTLPV VLDVGTNNPQ RLADPMYMGW RHPRITGPDY DNFVEEFMQA VQRRWPDALI
     QFEDFAQKNA MPLLERYKNR VCCFNDDIQG TAAVTVGSLL AACKAAGSQL SQQRITFLGA
     GSAGCGIAEA IIAQMVSEGI SDEQARSQVY MVDRWGLLEE GMPNLLDFQQ RLVQKKANTQ
     HWTTENNGYS LHDVIRNAKP TVLVGVSGAP GLFSEEIIKE MHQHCPRPIV FPLSNPTSRV
     EALPSDIIRW TNGEALVATG SPFDPVLHEG KTYPIVQCNN SYIFPGIGLG VLAANARRVT
     DEMLMESSRA LASCSPLAIN GHGPLLPPLE SIHSVSKKIA FAVAKKAIEQ GVAPEVTDEA
     LEASIEQHFW QPVYRRYKRT AF
//