Reviewed, UniProtKB/Swiss-Prot A5F1Z0 (MAO1_VIBC3)

Names and origin

Protein names

Recommended name:
    NAD-dependent malic enzyme
      Short name=NAD-ME
    EC=1.1.1.38

Gene names

Name:	sfcA
Ordered Locus Names:	VC0395_A0809

Organism

Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) [Complete proteome] [HAMAP]

Taxonomic identifier

345073 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD+ = pyruvate + CO2 + NADH. HAMAP MF_01619
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Ligand	Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 562	562	NAD-dependent malic enzyme HAMAP MF_01619		PRO_0000323541
Sites
	Active site	101	1	Proton donor By similarity
	Active site	172	1	Proton acceptor By similarity
	Metal binding	243	1	Divalent metal cation By similarity
	Metal binding	244	1	Divalent metal cation By similarity
	Metal binding	267	1	Divalent metal cation By similarity
	Binding site	154	1	NAD By similarity
	Binding site	267	1	NAD By similarity
	Binding site	415	1	NAD By similarity
	Site	267	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5F1Z0-1 [UniParc]. Last modified March 18, 2008. Version 2. Checksum: 934D1EEF53832130	FASTA	562	62,153
	10 20 30 40 50 60 MNNDKRPLYI SYAGPALLST PLLNKGSAFS AEERASFNLE GLLPEATETI QEQVVRAYQQ 70 80 90 100 110 120 YRGFESDMDK HIYLRNIQDT NETLFYRLVQ NHISEMMPII YTPTVGAACE NFSNIYRRGR 130 140 150 160 170 180 GLFISYANRD RIDDLLNNAA NHNVKVIVVT DGERILGLGD QGIGGMGIPI GKLSLYTACG 190 200 210 220 230 240 GISPAYTLPV VLDVGTNNPQ RLADPMYMGW RHPRITGPDY DNFVEEFMQA VQRRWPDALI 250 260 270 280 290 300 QFEDFAQKNA MPLLERYKNR VCCFNDDIQG TAAVTVGSLL AACKAAGSQL SQQRITFLGA 310 320 330 340 350 360 GSAGCGIAEA IIAQMVSEGI SDEQARSQVY MVDRWGLLEE GMPNLLDFQQ RLVQKKANTQ 370 380 390 400 410 420 HWTTENNGYS LHDVIRNAKP TVLVGVSGAP GLFSEEIIKE MHQHCPRPIV FPLSNPTSRV 430 440 450 460 470 480 EALPSDIIRW TNGEALVATG SPFDPVLHEG KTYPIVQCNN SYIFPGIGLG VLAANARRVT 490 500 510 520 530 540 DEMLMESSRA LASCSPLAIN GHGPLLPPLE SIHSVSKKIA FAVAKKAIEQ GVAPEVTDEA 550 560 LEASIEQHFW QPVYRRYKRT AF

References

[1]	Heidelberg J. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000627 Genomic DNA. Translation: ABQ21323.1. Different initiation.
RefSeq	YP_001216759.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5136256.
GenomeReviews	Gene locus VC0395_A0809 in contig CP000627_GR.
KEGG	vco:VC0395_A0809.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01619. [Tree]
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAO1_VIBC3

Accession

Primary (citable) accession number: A5F1Z0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	March 18, 2008
Last modified:	June 16, 2009
This is version 19 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)