Reviewed, UniProtKB/Swiss-Prot A5F1V4 (TPMT_VIBC3)

Names and origin

Protein names

Recommended name:
    Thiopurine S-methyltransferase
    EC=2.1.1.67
Alternative name(s):
    Thiopurine methyltransferase

Gene names

Name:	tpm
Ordered Locus Names:	VC0395_A0849, VC395_1345

Organism

Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) [Complete proteome] [HAMAP]

Taxonomic identifier

345073 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-methionine + a thiopurine = S-adenosyl-L-homocysteine + a thiopurine S-methylether. HAMAP MF_00812
Subcellular location	Cytoplasm By similarity HAMAP MF_00812.
Sequence similarities	Belongs to the methyltransferase superfamily. TPMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiopurine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 218	218	Thiopurine S-methyltransferase HAMAP MF_00812		PRO_1000072855
Sites
	Binding site	11	1	S-adenosyl-L-methionine By similarity
	Binding site	46	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	67	1	S-adenosyl-L-methionine By similarity
	Binding site	122	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5F1V4-1 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 5EBC09F292093640	FASTA	218	25,144
	10 20 30 40 50 60 MRDPEFWHNK WAANQIGFHL EDVNPLLIRF WSDLAPKRSE KVLVPLCGKS EDLIWLANQH 70 80 90 100 110 120 DSVQGVELSQ IAVRSFFAEH FYTPTVTRLN AQHELYQFDE LTLFTGDFFT APVESVDLVY 130 140 150 160 170 180 DRAALVALPE EMRTEYAQRV LQLLKPGGRI LLVSMDYVQT ELSGPPFSVP EAEIRTLFMG 190 200 210 CEVRRVYQDT SIDPHLNKRT QAGLSRFAEE VWVIEKSE

References

[1]	Heidelberg J. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"A recalibrated molecular clock and independent origins for the cholera pandemic clones." Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J., Wang W., Wang J., Qian W., Li D., Wang L. PLoS ONE 3:E4053-E4053(2008) [PubMed: 19115014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000627 Genomic DNA. Translation: ABQ22074.1. CP001235 Genomic DNA. Translation: ACP09353.1.
3D structure databases
SMR	A5F1V4. Positions 3-216.
ModBase	Search...
Protein-protein interaction databases
STRING	A5F1V4.
Genome annotation databases
GenomeReviews	Gene locus VC0395_A0849 in contig CP000627_GR.
KEGG	vco:VC0395_A0849.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0500.
HOGENOM	HBG444929.
OMA	GKSLDMC.
Family and domain databases
HAMAP	MF_00812. Thiopur_methtran. [Tree]
InterPro	IPR008854. Thiopurine_S-MeTrfase. IPR016822. Thiopurine_S-MeTrfase_sub. [Graphical view]
Pfam	PF05724. TPMT. 1 hit. [Graphical view]
PIRSF	PIRSF023956. Thiopurine_S-methyltransferase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPMT_VIBC3

Accession

Primary (citable) accession number: A5F1V4
Secondary accession number(s): C3LZY7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	June 12, 2007
Last modified:	February 9, 2010
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)