A5EZZ8 (SCRB_VIBC3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable sucrose-6-phosphate hydrolase
Short name=Sucrase
EC=3.2.1.26

Alternative name(s):
Invertase

Gene names

Name:	cscA
Ordered Locus Names:	VC0395_0599, VC395_A0657

Organism

Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) [Complete proteome] [HAMAP]

Taxonomic identifier

345073 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	546 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Enables the bacterium to metabolize sucrose as a sole carbon source By similarity.
Catalytic activity	Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides.
Pathway	Glycan biosynthesis; sucrose metabolism.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 32 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sucrose alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 546	546	Probable sucrose-6-phosphate hydrolase		PRO_0000341299
Regions
	Region	105 – 108	4	Substrate binding By similarity
	Region	167 – 168	2	Substrate binding By similarity
	Region	228 – 229	2	Substrate binding By similarity
Sites
	Active site	108	1	By similarity
	Binding site	124	1	Substrate By similarity
	Binding site	283	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5EZZ8 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 8F7360DCCEC6B2CC	FASTA	546	62,312
	10 20 30 40 50 60 MLLDTLLELA GGINNVTRIL APQGQVVLAL KHPPHAPHLP DDVSLQSVLG EWQLSVQRTA 70 80 90 100 110 120 EVSDQQLAAI GKAIAERQKR ATLPYQTALD CPYRPQWHIS PPQGLLNDPN GFIYHQEEYH 130 140 150 160 170 180 LFYQWHPFAC EHKDKYWVHL KSLDLVHWQW QSVALTPSDW FDSHGVFSGH AVSHQQDLWL 190 200 210 220 230 240 FYTGNTRLGT ERQRQTMQCA ARMNANGEFE KLGPVIRCLP EGVTEHIRDP KVIYTQGKWQ 250 260 270 280 290 300 MLLGAQTLAH QGRLAVYHSD DLLHWHFDKL YGDELGDYGY MWECPDWFEL QGEAFFVFGP 310 320 330 340 350 360 QGIASANPHH TIEHQNRIFR ATQNAQGEIA LLQGWPLDEG FDFYAPQTAQ TADGRRVLCG 370 380 390 400 410 420 WMGLPDETQH PSCDQGWIHQ LTALRELEWR EGKIYQHPLR ELDTLQSEPH TLLLSDTVTE 430 440 450 460 470 480 LKTKSFDLQV TLPWGCELRL MQNAQYCVTL TLDAENRLLR LDRSATQIRQ GDTIRELKLD 490 500 510 520 530 540 SPTVELRILA DQSSLEIFIN QGEHVMTSRI FTPLDATGIS LHGASVDAKL YYMAPASAPF NLEVNV

References

[1]	Heidelberg J. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39541 / Ogawa 395 / O395.
[2]	"A recalibrated molecular clock and independent origins for the cholera pandemic clones." Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J., Wang W., Wang J., Qian W., Li D., Wang L. PLoS ONE 3:E4053-E4053(2008) [PubMed: 19115014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39541 / Ogawa 395 / O395.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000626 Genomic DNA. Translation: ABQ18842.1. CP001236 Genomic DNA. Translation: ACP11491.1.
3D structure databases
ProteinModelPortal	A5EZZ8.
ModBase	Search...
Protein-protein interaction databases
STRING	A5EZZ8.
Protein family/group databases
CAZy	GH32. Glycoside Hydrolase Family 32.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GenomeReviews	Gene locus VC0395_0599 in contig CP000626_GR. Gene locus VC395_A0657 in contig CP001236_GR.
KEGG	vco:VC0395_0599.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1621.
HOGENOM	HBG693902.
OMA	HLFYQWF.
ProtClustDB	CLSK788970.
Enzyme and pathway databases
BioCyc	VCHO345073:VC0395_0599-MONOMER.
Family and domain databases
InterPro	IPR008985. ConA-like_lec_gl. IPR001362. Glyco_hydro_32. IPR018053. Glyco_hydro_32_AS. IPR013189. Glyco_hydro_32_C. IPR013148. Glyco_hydro_32_N. IPR023296. Glyco_hydro_43_beta-prop. IPR006232. Suc6P_hydrolase. [Graphical view]
Gene3D	G3DSA:2.115.10.20. Glyco_hydro_43_beta-prop. 1 hit.
KO	K01193.
Pfam	PF08244. Glyco_hydro_32C. 1 hit. PF00251. Glyco_hydro_32N. 1 hit. [Graphical view]
SMART	SM00640. Glyco_32. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit. SSF75005. Glyco_hydro_43_beta-prop. 1 hit.
TIGRFAMs	TIGR01322. ScrB_fam. 1 hit.
PROSITE	PS00609. GLYCOSYL_HYDROL_F32. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SCRB_VIBC3

Accession

Primary (citable) accession number: A5EZZ8
Secondary accession number(s): C3M5S8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	June 12, 2007
Last modified:	November 16, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program