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A5EXA7

- MIAB_DICNV

UniProt

A5EXA7 - MIAB_DICNV

Protein

tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase

Gene

miaB

Organism
Dichelobacter nodosus (strain VCS1703A)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 1 (12 Jun 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine.UniRule annotation

    Catalytic activityi

    N(6)-dimethylallyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 2-methylthio-N(6)-dimethylallyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi15 – 151Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi52 – 521Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi86 – 861Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi160 – 1601Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi164 – 1641Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi167 – 1671Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. iron ion binding Source: UniProtKB-HAMAP
    3. transferase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. tRNA modification Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciDNOD246195:GHHS-1242-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseUniRule annotation (EC:2.8.4.3UniRule annotation)
    Alternative name(s):
    (Dimethylallyl)adenosine tRNA methylthiotransferase MiaBUniRule annotation
    tRNA-i(6)A37 methylthiotransferaseUniRule annotation
    Gene namesi
    Name:miaBUniRule annotation
    Ordered Locus Names:DNO_1245
    OrganismiDichelobacter nodosus (strain VCS1703A)
    Taxonomic identifieri246195 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaCardiobacterialesCardiobacteriaceaeDichelobacter
    ProteomesiUP000000248: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 456456tRNA-2-methylthio-N(6)-dimethylallyladenosine synthasePRO_0000374269Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi246195.DNO_1245.

    Structurei

    3D structure databases

    ProteinModelPortaliA5EXA7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini6 – 123118MTTase N-terminalUniRule annotationAdd
    BLAST
    Domaini381 – 44464TRAMUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the methylthiotransferase family. MiaB subfamily.UniRule annotation
    Contains 1 MTTase N-terminal domain.UniRule annotation
    Contains 1 TRAM domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0621.
    HOGENOMiHOG000224767.
    KOiK06168.
    OMAiIEVYKDT.
    OrthoDBiEOG6P5ZD8.

    Family and domain databases

    Gene3Di3.80.30.20. 1 hit.
    HAMAPiMF_01864. tRNA_metthiotr_MiaB.
    InterProiIPR006638. Elp3/MiaB/NifB.
    IPR023970. MeThioTfrase/rSAM.
    IPR005839. Methylthiotransferase.
    IPR020612. Methylthiotransferase_CS.
    IPR013848. Methylthiotransferase_N.
    IPR006463. MiaB_methiolase.
    IPR007197. rSAM.
    IPR023404. rSAM_horseshoe.
    IPR002792. TRAM_dom.
    [Graphical view]
    PANTHERiPTHR11918. PTHR11918. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    PF01938. TRAM. 1 hit.
    PF00919. UPF0004. 1 hit.
    [Graphical view]
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
    PROSITEiPS51449. MTTASE_N. 1 hit.
    PS01278. MTTASE_RADICAL. 1 hit.
    PS50926. TRAM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A5EXA7-1 [UniParc]FASTAAdd to Basket

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    MQTVLKHVYI ETYGCQMNEY DSSKMLAVLK NSHGITPVAT PEEADVLLLN    50
    TCSVREKAQE KVFSQLGRWK SLKERKPHLI IGVGGCVASQ EGEMIRRRAP 100
    EVDVVFGPQT LHRLPNLIEE AQRSRGGVVD VSFPEIEKFD HLPEPRAEGP 150
    TAYVSVMEGC SKYCTYCVVP YTRGAEISRP FDDVLAECAT LAAQGVREIN 200
    LLGQNVNAYR GAMHDGTIAD LALLIEYVAA IPNIGRIRFT TSHPSEFSDA 250
    LIETYRRVPK LVSHLHLPVQ SGSNRILALM KRDYKVAEYQ EKLAKIRAIR 300
    PDISFSSDFI VGFPGEEEED FQATMDLIEA VFFDTSYSFI YSQRPGTPAS 350
    TMPDRVPLTV KKERLARLQA RILEMAASIS EAMVGTEQWV LVDRLSRKSE 400
    REVSGRTENN RVVNFSAPAS LIGRFAKVQI TAAYKNSLRG RLIEAELLPD 450
    PVVYTR 456
    Length:456
    Mass (Da):51,013
    Last modified:June 12, 2007 - v1
    Checksum:iB20D924B8494970D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000513 Genomic DNA. Translation: ABQ14300.1.
    RefSeqiYP_001210121.1. NC_009446.1.

    Genome annotation databases

    EnsemblBacteriaiABQ14300; ABQ14300; DNO_1245.
    GeneIDi5122130.
    KEGGidno:DNO_1245.
    PATRICi21786844. VBIDicNod48475_1238.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000513 Genomic DNA. Translation: ABQ14300.1 .
    RefSeqi YP_001210121.1. NC_009446.1.

    3D structure databases

    ProteinModelPortali A5EXA7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 246195.DNO_1245.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABQ14300 ; ABQ14300 ; DNO_1245 .
    GeneIDi 5122130.
    KEGGi dno:DNO_1245.
    PATRICi 21786844. VBIDicNod48475_1238.

    Phylogenomic databases

    eggNOGi COG0621.
    HOGENOMi HOG000224767.
    KOi K06168.
    OMAi IEVYKDT.
    OrthoDBi EOG6P5ZD8.

    Enzyme and pathway databases

    BioCyci DNOD246195:GHHS-1242-MONOMER.

    Family and domain databases

    Gene3Di 3.80.30.20. 1 hit.
    HAMAPi MF_01864. tRNA_metthiotr_MiaB.
    InterProi IPR006638. Elp3/MiaB/NifB.
    IPR023970. MeThioTfrase/rSAM.
    IPR005839. Methylthiotransferase.
    IPR020612. Methylthiotransferase_CS.
    IPR013848. Methylthiotransferase_N.
    IPR006463. MiaB_methiolase.
    IPR007197. rSAM.
    IPR023404. rSAM_horseshoe.
    IPR002792. TRAM_dom.
    [Graphical view ]
    PANTHERi PTHR11918. PTHR11918. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    PF01938. TRAM. 1 hit.
    PF00919. UPF0004. 1 hit.
    [Graphical view ]
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00089. TIGR00089. 1 hit.
    PROSITEi PS51449. MTTASE_N. 1 hit.
    PS01278. MTTASE_RADICAL. 1 hit.
    PS50926. TRAM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: VCS1703A.

    Entry informationi

    Entry nameiMIAB_DICNV
    AccessioniPrimary (citable) accession number: A5EXA7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: June 12, 2007
    Last modified: October 1, 2014
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3