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A5EXA7 (MIAB_DICNV) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase

EC=2.8.4.3
Alternative name(s):
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB
tRNA-i(6)A37 methylthiotransferase
Gene names
Name:miaB
Ordered Locus Names:DNO_1245
OrganismDichelobacter nodosus (strain VCS1703A) [Complete proteome] [HAMAP]
Taxonomic identifier246195 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaCardiobacterialesCardiobacteriaceaeDichelobacter

Protein attributes

Sequence length456 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine By similarity. HAMAP-Rule MF_01864

Catalytic activity

N(6)-dimethylallyladenine37 in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 2-methylthio-N(6)-dimethylallyladenine37 in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine. HAMAP-Rule MF_01864

Cofactor

Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Subunit structure

Monomer By similarity. HAMAP-Rule MF_01864

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_01864.

Sequence similarities

Belongs to the methylthiotransferase family. MiaB subfamily.

Contains 1 MTTase N-terminal domain.

Contains 1 TRAM domain.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   Ligand4Fe-4S
Iron
Iron-sulfur
Metal-binding
S-adenosyl-L-methionine
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtRNA modification

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function4 iron, 4 sulfur cluster binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

iron ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

transferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 456456tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase HAMAP-Rule MF_01864
PRO_0000374269

Regions

Domain6 – 123118MTTase N-terminal
Domain381 – 44464TRAM

Sites

Metal binding151Iron-sulfur (4Fe-4S) By similarity
Metal binding521Iron-sulfur (4Fe-4S) By similarity
Metal binding861Iron-sulfur (4Fe-4S) By similarity
Metal binding1601Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding1641Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding1671Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Sequences

Sequence LengthMass (Da)Tools
A5EXA7 [UniParc].

Last modified June 12, 2007. Version 1.
Checksum: B20D924B8494970D

FASTA45651,013
        10         20         30         40         50         60 
MQTVLKHVYI ETYGCQMNEY DSSKMLAVLK NSHGITPVAT PEEADVLLLN TCSVREKAQE 

        70         80         90        100        110        120 
KVFSQLGRWK SLKERKPHLI IGVGGCVASQ EGEMIRRRAP EVDVVFGPQT LHRLPNLIEE 

       130        140        150        160        170        180 
AQRSRGGVVD VSFPEIEKFD HLPEPRAEGP TAYVSVMEGC SKYCTYCVVP YTRGAEISRP 

       190        200        210        220        230        240 
FDDVLAECAT LAAQGVREIN LLGQNVNAYR GAMHDGTIAD LALLIEYVAA IPNIGRIRFT 

       250        260        270        280        290        300 
TSHPSEFSDA LIETYRRVPK LVSHLHLPVQ SGSNRILALM KRDYKVAEYQ EKLAKIRAIR 

       310        320        330        340        350        360 
PDISFSSDFI VGFPGEEEED FQATMDLIEA VFFDTSYSFI YSQRPGTPAS TMPDRVPLTV 

       370        380        390        400        410        420 
KKERLARLQA RILEMAASIS EAMVGTEQWV LVDRLSRKSE REVSGRTENN RVVNFSAPAS 

       430        440        450 
LIGRFAKVQI TAAYKNSLRG RLIEAELLPD PVVYTR 

« Hide

References

[1]"Genome sequence and identification of candidate vaccine antigens from the animal pathogen Dichelobacter nodosus."
Myers G.S.A., Parker D., Al-Hasani K., Kennan R.M., Seemann T., Ren Q., Badger J.H., Selengut J.D., Deboy R.T., Tettelin H., Boyce J.D., McCarl V.P., Han X., Nelson W.C., Madupu R., Mohamoud Y., Holley T., Fedorova N. expand/collapse author list , Khouri H., Bottomley S.P., Whittington R.J., Adler B., Songer J.G., Rood J.I., Paulsen I.T.
Nat. Biotechnol. 25:569-575(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: VCS1703A.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000513 Genomic DNA. Translation: ABQ14300.1.
RefSeqYP_001210121.1. NC_009446.1.

3D structure databases

ProteinModelPortalA5EXA7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING246195.DNO_1245.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABQ14300; ABQ14300; DNO_1245.
GeneID5122130.
KEGGdno:DNO_1245.
PATRIC21786844. VBIDicNod48475_1238.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0621.
HOGENOMHOG000224767.
KOK06168.
OMAIEVYKDT.
OrthoDBEOG6P5ZD8.

Enzyme and pathway databases

BioCycDNOD246195:GHHS-1242-MONOMER.

Family and domain databases

Gene3D3.80.30.20. 1 hit.
HAMAPMF_01864. tRNA_metthiotr_MiaB.
InterProIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERPTHR11918. PTHR11918. 1 hit.
PfamPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00089. TIGR00089. 1 hit.
PROSITEPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMIAB_DICNV
AccessionPrimary (citable) accession number: A5EXA7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: June 12, 2007
Last modified: May 14, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families