Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

A5EWT0

- SYI_DICNV

UniProt

A5EWT0 - SYI_DICNV

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Dichelobacter nodosus (strain VCS1703A)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei561 – 5611Aminoacyl-adenylateUniRule annotation
Binding sitei605 – 6051ATPUniRule annotation
Metal bindingi887 – 8871ZincUniRule annotation
Metal bindingi890 – 8901ZincUniRule annotation
Metal bindingi907 – 9071ZincUniRule annotation
Metal bindingi910 – 9101ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciDNOD246195:GHHS-83-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:DNO_0083
OrganismiDichelobacter nodosus (strain VCS1703A)
Taxonomic identifieri246195 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaCardiobacterialesCardiobacteriaceaeDichelobacter
ProteomesiUP000000248: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 924924Isoleucine--tRNA ligasePRO_1000022060Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi246195.DNO_0083.

Structurei

3D structure databases

ProteinModelPortaliA5EWT0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi602 – 6065"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5EWT0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MADYKHTLNL PQTDFPMRGN LAQREPEQLR WWQEHEIYRK QREAFADCPT
60 70 80 90 100
FMLHDGPPYA NGQIHVGHAL NKTLKDIIIK SRHLLGYNSP YVPGWDCHGL
110 120 130 140 150
PIEQKVEQKI GKPNQKVSAT EFRAACRRYA AEQVALQKDG FMRLGIFGFW
160 170 180 190 200
DQPYLTMNFE TEAQIVRALR DIVAAGHVTQ GFKPINWCFD CASSLAEAEV
210 220 230 240 250
EYQDKTSYSI DVAFAVKDVA ALAKIMHADV VPAAVDVVIW TTTPWTLPAN
260 270 280 290 300
VAVSIHPEFY YVLVEIEGWY CVVAEELIPA LKSRWKKSAD WRICGRALGK
310 320 330 340 350
DLDRLTLRHP FIDRDVLLIN GTHVTLDAGT GCVHTAPAHG VEDYDVCQQY
360 370 380 390 400
GIDLIHCVLG NGLYNDDTPH FAGQHIFAAE PQIIELLQTQ NRLIAVEKIT
410 420 430 440 450
HSYPHCWRHK TPTIYRATTQ WFISMDKAGL RQKALDGLNE VAFTPDWGKP
460 470 480 490 500
RLLNMIAHRP DWCISRQRYW GVPLCFVVDK QTGQLHPDIV NIMDKAASAI
510 520 530 540 550
EQKGVEAWYE LSLDTLLSGK EVDRYEKLND VLDVWFDSGT THYSVLKKRA
560 570 580 590 600
ELSYPADLYL EGSDQHRGWF HSSLLTASAI TGKPPYKALL THGFTVDEDG
610 620 630 640 650
RKMSKSLGNV VDPNQVIKTL GADILRLWVA SADYTAEVSL SPNILNQRAD
660 670 680 690 700
AYRRMRNTCR FLLANLHDFD PEKNSVAYEK LLPLDRYVIA VAHDLQEKIK
710 720 730 740 750
KLYVSYDFHL IYQEIFNFCS VMLGGFYLDV IKDRQYTVAQ NALARRSAQT
760 770 780 790 800
AIFHIIEAMV RWLAPILSFT AEEIWRYLPG KREESVFLTK FYEGLQPLND
810 820 830 840 850
DFLSMHDWEL LLQLREKVNA ALEKARNQGI IGGSLEAMVV LHLPPQEYEI
860 870 880 890 900
AARFEQELRF ILIVSAVNVQ PSAALSIEVQ HAIGEKCERC WHYLPDVGTD
910 920
HQHPTLCKRC IENVDGGGEL RRFA
Length:924
Mass (Da):105,346
Last modified:June 12, 2007 - v1
Checksum:iBB6A36F6ED0BBF2C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000513 Genomic DNA. Translation: ABQ13975.1.
RefSeqiYP_001209014.1. NC_009446.1.

Genome annotation databases

EnsemblBacteriaiABQ13975; ABQ13975; DNO_0083.
GeneIDi5123129.
KEGGidno:DNO_0083.
PATRICi21784452. VBIDicNod48475_0078.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000513 Genomic DNA. Translation: ABQ13975.1 .
RefSeqi YP_001209014.1. NC_009446.1.

3D structure databases

ProteinModelPortali A5EWT0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 246195.DNO_0083.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABQ13975 ; ABQ13975 ; DNO_0083 .
GeneIDi 5123129.
KEGGi dno:DNO_0083.
PATRICi 21784452. VBIDicNod48475_0078.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci DNOD246195:GHHS-83-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VCS1703A.

Entry informationi

Entry nameiSYI_DICNV
AccessioniPrimary (citable) accession number: A5EWT0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 12, 2007
Last modified: October 29, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3