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A5EWT0

- SYI_DICNV

UniProt

A5EWT0 - SYI_DICNV

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Protein
Isoleucine--tRNA ligase
Gene
ileS, DNO_0083
Organism
Dichelobacter nodosus (strain VCS1703A)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei561 – 5611Aminoacyl-adenylate By similarity
Binding sitei605 – 6051ATP By similarity
Metal bindingi887 – 8871Zinc By similarity
Metal bindingi890 – 8901Zinc By similarity
Metal bindingi907 – 9071Zinc By similarity
Metal bindingi910 – 9101Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciDNOD246195:GHHS-83-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:DNO_0083
OrganismiDichelobacter nodosus (strain VCS1703A)
Taxonomic identifieri246195 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaCardiobacterialesCardiobacteriaceaeDichelobacter
ProteomesiUP000000248: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 924924Isoleucine--tRNA ligaseUniRule annotation
PRO_1000022060Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi246195.DNO_0083.

Structurei

3D structure databases

ProteinModelPortaliA5EWT0.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotation
Add
BLAST
Motifi602 – 6065"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5EWT0-1 [UniParc]FASTAAdd to Basket

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MADYKHTLNL PQTDFPMRGN LAQREPEQLR WWQEHEIYRK QREAFADCPT    50
FMLHDGPPYA NGQIHVGHAL NKTLKDIIIK SRHLLGYNSP YVPGWDCHGL 100
PIEQKVEQKI GKPNQKVSAT EFRAACRRYA AEQVALQKDG FMRLGIFGFW 150
DQPYLTMNFE TEAQIVRALR DIVAAGHVTQ GFKPINWCFD CASSLAEAEV 200
EYQDKTSYSI DVAFAVKDVA ALAKIMHADV VPAAVDVVIW TTTPWTLPAN 250
VAVSIHPEFY YVLVEIEGWY CVVAEELIPA LKSRWKKSAD WRICGRALGK 300
DLDRLTLRHP FIDRDVLLIN GTHVTLDAGT GCVHTAPAHG VEDYDVCQQY 350
GIDLIHCVLG NGLYNDDTPH FAGQHIFAAE PQIIELLQTQ NRLIAVEKIT 400
HSYPHCWRHK TPTIYRATTQ WFISMDKAGL RQKALDGLNE VAFTPDWGKP 450
RLLNMIAHRP DWCISRQRYW GVPLCFVVDK QTGQLHPDIV NIMDKAASAI 500
EQKGVEAWYE LSLDTLLSGK EVDRYEKLND VLDVWFDSGT THYSVLKKRA 550
ELSYPADLYL EGSDQHRGWF HSSLLTASAI TGKPPYKALL THGFTVDEDG 600
RKMSKSLGNV VDPNQVIKTL GADILRLWVA SADYTAEVSL SPNILNQRAD 650
AYRRMRNTCR FLLANLHDFD PEKNSVAYEK LLPLDRYVIA VAHDLQEKIK 700
KLYVSYDFHL IYQEIFNFCS VMLGGFYLDV IKDRQYTVAQ NALARRSAQT 750
AIFHIIEAMV RWLAPILSFT AEEIWRYLPG KREESVFLTK FYEGLQPLND 800
DFLSMHDWEL LLQLREKVNA ALEKARNQGI IGGSLEAMVV LHLPPQEYEI 850
AARFEQELRF ILIVSAVNVQ PSAALSIEVQ HAIGEKCERC WHYLPDVGTD 900
HQHPTLCKRC IENVDGGGEL RRFA 924
Length:924
Mass (Da):105,346
Last modified:June 12, 2007 - v1
Checksum:iBB6A36F6ED0BBF2C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000513 Genomic DNA. Translation: ABQ13975.1.
RefSeqiWP_011927835.1. NC_009446.1.
YP_001209014.1. NC_009446.1.

Genome annotation databases

EnsemblBacteriaiABQ13975; ABQ13975; DNO_0083.
GeneIDi5123129.
KEGGidno:DNO_0083.
PATRICi21784452. VBIDicNod48475_0078.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000513 Genomic DNA. Translation: ABQ13975.1 .
RefSeqi WP_011927835.1. NC_009446.1.
YP_001209014.1. NC_009446.1.

3D structure databases

ProteinModelPortali A5EWT0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 246195.DNO_0083.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABQ13975 ; ABQ13975 ; DNO_0083 .
GeneIDi 5123129.
KEGGi dno:DNO_0083.
PATRICi 21784452. VBIDicNod48475_0078.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci DNOD246195:GHHS-83-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: VCS1703A.

Entry informationi

Entry nameiSYI_DICNV
AccessioniPrimary (citable) accession number: A5EWT0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 12, 2007
Last modified: September 3, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi