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A5EWT0

- SYI_DICNV

UniProt

A5EWT0 - SYI_DICNV

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Dichelobacter nodosus (strain VCS1703A)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 55 (01 Oct 2014)
      Sequence version 1 (12 Jun 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei561 – 5611Aminoacyl-adenylateUniRule annotation
    Binding sitei605 – 6051ATPUniRule annotation
    Metal bindingi887 – 8871ZincUniRule annotation
    Metal bindingi890 – 8901ZincUniRule annotation
    Metal bindingi907 – 9071ZincUniRule annotation
    Metal bindingi910 – 9101ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciDNOD246195:GHHS-83-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:DNO_0083
    OrganismiDichelobacter nodosus (strain VCS1703A)
    Taxonomic identifieri246195 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaCardiobacterialesCardiobacteriaceaeDichelobacter
    ProteomesiUP000000248: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 924924Isoleucine--tRNA ligasePRO_1000022060Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi246195.DNO_0083.

    Structurei

    3D structure databases

    ProteinModelPortaliA5EWT0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi602 – 6065"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A5EWT0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADYKHTLNL PQTDFPMRGN LAQREPEQLR WWQEHEIYRK QREAFADCPT    50
    FMLHDGPPYA NGQIHVGHAL NKTLKDIIIK SRHLLGYNSP YVPGWDCHGL 100
    PIEQKVEQKI GKPNQKVSAT EFRAACRRYA AEQVALQKDG FMRLGIFGFW 150
    DQPYLTMNFE TEAQIVRALR DIVAAGHVTQ GFKPINWCFD CASSLAEAEV 200
    EYQDKTSYSI DVAFAVKDVA ALAKIMHADV VPAAVDVVIW TTTPWTLPAN 250
    VAVSIHPEFY YVLVEIEGWY CVVAEELIPA LKSRWKKSAD WRICGRALGK 300
    DLDRLTLRHP FIDRDVLLIN GTHVTLDAGT GCVHTAPAHG VEDYDVCQQY 350
    GIDLIHCVLG NGLYNDDTPH FAGQHIFAAE PQIIELLQTQ NRLIAVEKIT 400
    HSYPHCWRHK TPTIYRATTQ WFISMDKAGL RQKALDGLNE VAFTPDWGKP 450
    RLLNMIAHRP DWCISRQRYW GVPLCFVVDK QTGQLHPDIV NIMDKAASAI 500
    EQKGVEAWYE LSLDTLLSGK EVDRYEKLND VLDVWFDSGT THYSVLKKRA 550
    ELSYPADLYL EGSDQHRGWF HSSLLTASAI TGKPPYKALL THGFTVDEDG 600
    RKMSKSLGNV VDPNQVIKTL GADILRLWVA SADYTAEVSL SPNILNQRAD 650
    AYRRMRNTCR FLLANLHDFD PEKNSVAYEK LLPLDRYVIA VAHDLQEKIK 700
    KLYVSYDFHL IYQEIFNFCS VMLGGFYLDV IKDRQYTVAQ NALARRSAQT 750
    AIFHIIEAMV RWLAPILSFT AEEIWRYLPG KREESVFLTK FYEGLQPLND 800
    DFLSMHDWEL LLQLREKVNA ALEKARNQGI IGGSLEAMVV LHLPPQEYEI 850
    AARFEQELRF ILIVSAVNVQ PSAALSIEVQ HAIGEKCERC WHYLPDVGTD 900
    HQHPTLCKRC IENVDGGGEL RRFA 924
    Length:924
    Mass (Da):105,346
    Last modified:June 12, 2007 - v1
    Checksum:iBB6A36F6ED0BBF2C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000513 Genomic DNA. Translation: ABQ13975.1.
    RefSeqiWP_011927835.1. NC_009446.1.
    YP_001209014.1. NC_009446.1.

    Genome annotation databases

    EnsemblBacteriaiABQ13975; ABQ13975; DNO_0083.
    GeneIDi5123129.
    KEGGidno:DNO_0083.
    PATRICi21784452. VBIDicNod48475_0078.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000513 Genomic DNA. Translation: ABQ13975.1 .
    RefSeqi WP_011927835.1. NC_009446.1.
    YP_001209014.1. NC_009446.1.

    3D structure databases

    ProteinModelPortali A5EWT0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 246195.DNO_0083.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABQ13975 ; ABQ13975 ; DNO_0083 .
    GeneIDi 5123129.
    KEGGi dno:DNO_0083.
    PATRICi 21784452. VBIDicNod48475_0078.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci DNOD246195:GHHS-83-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: VCS1703A.

    Entry informationi

    Entry nameiSYI_DICNV
    AccessioniPrimary (citable) accession number: A5EWT0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: June 12, 2007
    Last modified: October 1, 2014
    This is version 55 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3