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A5EWD6 (SYL_DICNV) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:DNO_0246
OrganismDichelobacter nodosus (strain VCS1703A) [Complete proteome] [HAMAP]
Taxonomic identifier246195 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaCardiobacterialesCardiobacteriaceaeDichelobacter

Protein attributes

Sequence length867 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 867867Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009336

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif631 – 6355"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6341ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A5EWD6 [UniParc].

Last modified June 12, 2007. Version 1.
Checksum: E394F561913ABF7D

FASTA86799,087
        10         20         30         40         50         60 
MQDTYQPAQI EAAIQQQWAN EERFKAVVNP QKEKFYCLSM FPYPSGKLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VISRYQRMLG KNVLQPMGWD AFGMPAENAA MKNQVAPAQW TYQNIAEMKK QLQSLGFAID 

       130        140        150        160        170        180 
WSREITTCRP DYYRWEQWLF TRLYQKGVIY RKLGAVNWDP VDQTVLANEQ VIDGRGWRSG 

       190        200        210        220        230        240 
AIVEKREIPM YYFRITDYAE ELLNDLEQLD GWPERVKMMQ RNWIGKSRGM TIRFPIEPES 

       250        260        270        280        290        300 
QKGLSAENAQ FLQIYTTRPD TIFGVTFLAV AAEHPLALAA AEDNPALRQF MTECKTGSVA 

       310        320        330        340        350        360 
EADLATMPKK GMATGRFVTH PLTGKRLPVW VGNYVLSGYG DAAVMGVPAH DERDFHFAHQ 

       370        380        390        400        410        420 
YGLPIVQVIQ TEKDLPAFNA DSWQEWYGDK EEMHVIASDD CNGLNYDQAF EKLSEKLAAL 

       430        440        450        460        470        480 
NLGEPKTQYR LRDWGISRQR YWGCPIPIIH HDDGEALADT LPVVLPEDKI PDGGGSVLAQ 

       490        500        510        520        530        540 
SPDYYECRFQ GKAARRETDT MDTFVESSWY QFRYMSPHYQ KGMLDPEEVA YWQDVDQYIG 

       550        560        570        580        590        600 
GIEHAILHLL YARFFTKLLR DEGLVHTDEP FKKLLTQGMV LAGTWYREQE HGSKIWFNPA 

       610        620        630        640        650        660 
DVQVKTDDKG RIIGGVLLSD GKPVQYGGME KMSKSKNNGV DPQALIKTYG ADTARLYTMF 

       670        680        690        700        710        720 
TAPPEASLEW SESGVEGAYR FLRRLWAYCY EFRHSIAKPS EVVPLNEKHQ NIRREIHEQL 

       730        740        750        760        770        780 
RAARFDYERN QFNTVVSAGM KLFNCLNEIE PDYDALRREG VRILLLILSP IVPHITETLW 

       790        800        810        820        830        840 
QALDFSGVIS DQALPEVDET ALKQDNMTLV VQINGKRRGE ISVATDAAQQ NIIDCALADE 

       850        860 
RFAPYLAGLT VQKTIYVPKK LVNIVAK 

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References

[1]"Genome sequence and identification of candidate vaccine antigens from the animal pathogen Dichelobacter nodosus."
Myers G.S.A., Parker D., Al-Hasani K., Kennan R.M., Seemann T., Ren Q., Badger J.H., Selengut J.D., Deboy R.T., Tettelin H., Boyce J.D., McCarl V.P., Han X., Nelson W.C., Madupu R., Mohamoud Y., Holley T., Fedorova N. expand/collapse author list , Khouri H., Bottomley S.P., Whittington R.J., Adler B., Songer J.G., Rood J.I., Paulsen I.T.
Nat. Biotechnol. 25:569-575(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: VCS1703A.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000513 Genomic DNA. Translation: ABQ13397.1.
RefSeqYP_001209169.1. NC_009446.1.

3D structure databases

ProteinModelPortalA5EWD6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING246195.DNO_0246.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABQ13397; ABQ13397; DNO_0246.
GeneID5122373.
KEGGdno:DNO_0246.
PATRIC21784792. VBIDicNod48475_0245.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycDNOD246195:GHHS-246-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_DICNV
AccessionPrimary (citable) accession number: A5EWD6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 12, 2007
Last modified: April 16, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries