A5EW23 (KDSA_DICNV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-dehydro-3-deoxyphosphooctonate aldolase
EC=2.5.1.55

Alternative name(s):
3-deoxy-D-manno-octulosonic acid 8-phosphate synthase
KDO-8-phosphate synthase
Short name=KDO 8-P synthase
Short name=KDOPS
Phospho-2-dehydro-3-deoxyoctonate aldolase

Gene names

Name:	kdsA
Ordered Locus Names:	DNO_0361

Organism

Dichelobacter nodosus (strain VCS1703A) [Complete proteome] [HAMAP]

Taxonomic identifier

246195 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Cardiobacteriales › Cardiobacteriaceae › Dichelobacter

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + D-arabinose 5-phosphate + H2O = 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate. HAMAP MF_00056
Pathway	Carbohydrate biosynthesis; 3-deoxy-D-manno-octulosonate biosynthesis; 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate: step 2/3. HAMAP MF_00056 Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. HAMAP MF_00056
Subcellular location	Cytoplasm By similarity HAMAP MF_00056.
Sequence similarities	Belongs to the KdsA family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-deoxy-8-phosphooctulonate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 277	277	2-dehydro-3-deoxyphosphooctonate aldolase HAMAP MF_00056		PRO_1000117775

Sequences

	Sequence		Length	Mass (Da)
	A5EW23 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 0230E216F36CEFAB	FASTA	277	30,177
	10 20 30 40 50 60 MKLIDFDVDN DRPFFLIAGP CVIESEYLAL HTAEVLKTIT DELKIPFIYK SSFDKANRSS 70 80 90 100 110 120 GASFRGLGLA EGLRILAKVK ETFAVPILTD VHEYTPLDEV ASVVDVLQTP AFLCRQTDFI 130 140 150 160 170 180 TRVCAQGKPV NIKKGQFLAP WDMKHVVAKA KATGNEQIML CERGASFGYN NLVADMRSLA 190 200 210 220 230 240 VMKTFSAPVV FDATHSVQLP GGNGASSGGQ REFVPVLARA AIAVGVAGIF METHPEPEKA 250 260 270 LSDGANSIPL AQMRHLLQQL RALDTLVKTN ELMKELS

References

[1]

"Genome sequence and identification of candidate vaccine antigens from the animal pathogen Dichelobacter nodosus." Myers G.S.A., Parker D., Al-Hasani K., Kennan R.M., Seemann T., Ren Q., Badger J.H., Selengut J.D., Deboy R.T., Tettelin H., Boyce J.D., McCarl V.P., Han X., Nelson W.C., Madupu R., Mohamoud Y., Holley T., Fedorova N. , Khouri H., Bottomley S.P., Whittington R.J., Adler B., Songer J.G., Rood J.I., Paulsen I.T. Nat. Biotechnol. 25:569-575(2007) [PubMed: 17468768] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VCS1703A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000513 Genomic DNA. Translation: ABQ14162.1.
RefSeq	YP_001209280.1. NC_009446.1.
3D structure databases
ProteinModelPortal	A5EW23.
SMR	A5EW23. Positions 13-270.
ModBase	Search...
Protein-protein interaction databases
STRING	A5EW23.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5122293.
GenomeReviews	Gene locus DNO_0361 in contig CP000513_GR.
KEGG	dno:DNO_0361.
PATRIC	21785022. VBIDicNod48475_0359.
TIGR	DNO_0361.
Phylogenomic databases
eggNOG	COG2877.
HOGENOM	HBG575990.
OMA	DVHEAAQ.
ProtClustDB	PRK05198.
Enzyme and pathway databases
BioCyc	DNOD246195:DNO_0361-MONOMER.
Family and domain databases
HAMAP	MF_00056. KDO8P_synth. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR006218. DAHP1/KDSA. IPR006269. KDO8P_synthase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01627.
PANTHER	PTHR21057:SF2. KDO8P_synthase. 1 hit.
Pfam	PF00793. DAHP_synth_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01362. KDO8P_synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDSA_DICNV

Accession

Primary (citable) accession number: A5EW23

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	June 12, 2007
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program