A5EV80 (SERC_DICNV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoserine aminotransferase
EC=2.6.1.52

Alternative name(s):
Phosphohydroxythreonine aminotransferase
Short name=PSAT

Gene names

Name:	serC
Ordered Locus Names:	DNO_0670

Organism

Dichelobacter nodosus (strain VCS1703A) [Complete proteome] [HAMAP]

Taxonomic identifier

246195 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Cardiobacteriales › Cardiobacteriaceae › Dichelobacter

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine By similarity. HAMAP MF_00160
Catalytic activity	O-phospho-L-serine + 2-oxoglutarate = 3-phosphonooxypyruvate + L-glutamate. HAMAP MF_00160 4-phosphonooxy-L-threonine + 2-oxoglutarate = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + L-glutamate. HAMAP MF_00160
Cofactor	Binds 1 pyridoxal phosphate per subunit By similarity. HAMAP MF_00160
Pathway	Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 2/3. HAMAP MF_00160 Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 3/5. HAMAP MF_00160
Subunit structure	Homodimer By similarity. HAMAP MF_00160
Subcellular location	Cytoplasm By similarity HAMAP MF_00160.
Sequence similarities	Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Pyridoxine biosynthesis Serine biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW pyridoxine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	O-phospho-L-serine:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 358	358	Phosphoserine aminotransferase HAMAP MF_00160		PRO_1000058210
Regions
	Region	236 – 237	2	Pyridoxal phosphate binding By similarity
Sites
	Binding site	43	1	L-glutamate By similarity
	Binding site	103	1	Pyridoxal phosphate By similarity
	Binding site	153	1	Pyridoxal phosphate By similarity
	Binding site	172	1	Pyridoxal phosphate By similarity
	Binding site	195	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	196	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5EV80 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 952FF80E22CA56B8	FASTA	358	40,069
	10 20 30 40 50 60 MSKRVFNFYP GPCTLPLPVL QQAQKELLDF EGCGMSVMEI SHRSQRFEAI LAETLSLAKK 70 80 90 100 110 120 LIGAPDDFCV LLIAGGAHQQ FAMTALNLLA DGGSAGIVNS GLWAKRALEE AQRVGKMVEL 130 140 150 160 170 180 WRAPDGKCTT LPDLKTLTVP KNLRYVHLTS NETVDGLQFP ELPDLGVPLV LDVSSDYYTR 190 200 210 220 230 240 PLPWDYCDIV YGGVQKNLAP SGMALVFVRK QCLREHTNLA RFFCYKHHAD ANSLLNTPPT 250 260 270 280 290 300 WQIYILHLVL KWIEQQGGVA HFAALAQKRS AKLYDFIDNN DFYRNDVEKK YRSKINVVMR 310 320 330 340 350 TPSDALDTQF WQEAETHALV GLKGHSAVGG LRASLYNAME MAGVEALIDF MHDFAQRH

References

[1]

"Genome sequence and identification of candidate vaccine antigens from the animal pathogen Dichelobacter nodosus." Myers G.S.A., Parker D., Al-Hasani K., Kennan R.M., Seemann T., Ren Q., Badger J.H., Selengut J.D., Deboy R.T., Tettelin H., Boyce J.D., McCarl V.P., Han X., Nelson W.C., Madupu R., Mohamoud Y., Holley T., Fedorova N. , Khouri H., Bottomley S.P., Whittington R.J., Adler B., Songer J.G., Rood J.I., Paulsen I.T. Nat. Biotechnol. 25:569-575(2007) [PubMed: 17468768] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: VCS1703A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000513 Genomic DNA. Translation: ABQ13312.1.
RefSeq	YP_001209579.1. NC_009446.1.
3D structure databases
ProteinModelPortal	A5EV80.
ModBase	Search...
Protein-protein interaction databases
STRING	A5EV80.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5122979.
GenomeReviews	Gene locus DNO_0670 in contig CP000513_GR.
KEGG	dno:DNO_0670.
PATRIC	21785662. VBIDicNod48475_0674.
TIGR	DNO_0670.
Phylogenomic databases
eggNOG	COG1932.
HOGENOM	HBG289982.
OMA	YEVLFLQ.
ProtClustDB	PRK05355.
Enzyme and pathway databases
BioCyc	DNOD246195:DNO_0670-MONOMER.
Family and domain databases
HAMAP	MF_00160. SerC_aminotrans_5. [Tree]
InterPro	IPR000192. Aminotrans_V/Cys_dSase. IPR022278. Pser_aminoTfrase. IPR003248. Pser_aminoTfrase_subgr. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
KO	K00831.
PANTHER	PTHR21152:SF1. PTHR21152:SF1. 1 hit.
Pfam	PF00266. Aminotran_5. 1 hit. [Graphical view]
PIRSF	PIRSF000525. SerC. 1 hit.
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITE	PS00595. AA_TRANSFER_CLASS_5. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SERC_DICNV

Accession

Primary (citable) accession number: A5EV80

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	June 12, 2007
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program