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A5ETJ9

- GLND_BRASB

UniProt

A5ETJ9 - GLND_BRASB

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Protein
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Gene
glnD, BBta_7648
Organism
Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen fixation and metabolism By similarity.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. metal ion binding Source: InterPro
  4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen fixation Source: UniProtKB-HAMAP
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciBSP288000:GJBR-7337-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Short name:
UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein
Including the following 2 domains:
[Protein-PII] uridylyltransferase (EC:2.7.7.59)
Short name:
PII uridylyltransferase
Short name:
UTase
[Protein-PII]-UMP uridylyl-removing enzyme (EC:3.1.4.-)
Short name:
UR
Gene namesi
Name:glnD
Ordered Locus Names:BBta_7648
OrganismiBradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182)
Taxonomic identifieri288000 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
ProteomesiUP000000246: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 931931Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
PRO_1000022327Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi288000.BBta_7648.

Structurei

3D structure databases

ProteinModelPortaliA5ETJ9.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini523 – 59977HD
Add
BLAST
Domaini740 – 82283ACT 1
Add
BLAST
Domaini851 – 93181ACT 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 383383UridylyltransferaseUniRule annotation
Add
BLAST
Regioni384 – 739356Uridylyl-removingUniRule annotation
Add
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.
Contains 2 ACT domains.
Contains 1 HD domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261779.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5ETJ9-1 [UniParc]FASTAAdd to Basket

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MDSVTPNSRP ESFPEFDSAG LAAAVDALAA QHSGREDMFR AAVVQLLKAE    50
LVKARAVAQA QLLKDRHGRR CAERLCFVQD EIIRILYAAA TQHLYRSQVP 100
SGAERMAVVA TGGYGRGLMA PESDIDLLFI LPYKQTAWGE QVAEAILYSL 150
WDMGLKVGHA TRSVDESIRQ ARGDMTIRTA ILETRYLAGD RPLYDELVER 200
FDTEVVQGTA AEFVAAKLAE REERHRRGGQ SRYLVEPNVK DGKGGLRDLH 250
TLFWIAKYVY RVRETAELVE RGVFDAHEYR TFRRCADFLW SVRCNLHFVS 300
GRPEERLSFD LQREIAVRLG YTSHPGMQDV ERFMKHYFLV AKEVGNLTAI 350
LCAKLEDQQA KPAPVLSRVI SRLKTGNSWR RVPESDDFIV DNNRINLAAP 400
DVFKHDPVNL IRIFRLAQKN NLAFHPDAMR AVTRSLNLIN TELRDNPDAN 450
RLFMEILTSN DAETVLRRMN ETGVLGHFIR AFGRIVSMMQ FNMYHHYTVD 500
EHLIRCIGFL QEIERGGIDE FALASDLMRK IRPEHRAVIY ISVLLHDVAK 550
GRPEDHSIAG AKVARRLCPR LGFNNADTEL VAWLIEEHLT MSTVAQSRDL 600
SDRRTIEKFA AVVQSVEQMK LLTILTTADI RGVGPGVWNG WKAQLLRTLY 650
YETEPVLTGG FSEVNRAKRI TAAQAEFRNA FTDWPEDELN TYIGRHYPAY 700
WLKVELPRKI RHARFVRASE DAGHKLAINV GFDPARGVTE LTIFAMDHPW 750
LLSIIAGACA SAGANIVDAQ IYTTTDGRAL DTIAISREYE RDEDEGRRAT 800
RIGETIEQVL EGKLRLPDAV ARRTTRGKQH KAFSVEPEVS INNQWSELYT 850
VIEVSGLDRP GLLYELTTAI SKLNLNIASA HVATFGERAR DVFYVTDLLG 900
AQINAPTRQA AIKSALLHLL ASDDTAAQPA A 931
Length:931
Mass (Da):104,895
Last modified:June 12, 2007 - v1
Checksum:i2C910DCB195E85E3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000494 Genomic DNA. Translation: ABQ39493.1.
RefSeqiWP_012047384.1. NC_009485.1.
YP_001243399.1. NC_009485.1.

Genome annotation databases

EnsemblBacteriaiABQ39493; ABQ39493; BBta_7648.
GeneIDi5149882.
KEGGibbt:BBta_7648.
PATRICi21215907. VBIBraSp29847_7601.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000494 Genomic DNA. Translation: ABQ39493.1 .
RefSeqi WP_012047384.1. NC_009485.1.
YP_001243399.1. NC_009485.1.

3D structure databases

ProteinModelPortali A5ETJ9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 288000.BBta_7648.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABQ39493 ; ABQ39493 ; BBta_7648 .
GeneIDi 5149882.
KEGGi bbt:BBta_7648.
PATRICi 21215907. VBIBraSp29847_7601.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261779.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci BSP288000:GJBR-7337-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BTAi1 / ATCC BAA-1182.

Entry informationi

Entry nameiGLND_BRASB
AccessioniPrimary (citable) accession number: A5ETJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 12, 2007
Last modified: September 3, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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