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A5ETJ9

- GLND_BRASB

UniProt

A5ETJ9 - GLND_BRASB

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen fixation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen fixation Source: UniProtKB-HAMAP
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciBSP288000:GJBR-7337-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:BBta_7648
OrganismiBradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182)
Taxonomic identifieri288000 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
ProteomesiUP000000246: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 931931Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_1000022327Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi288000.BBta_7648.

Structurei

3D structure databases

ProteinModelPortaliA5ETJ9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini523 – 59977HDUniRule annotationAdd
BLAST
Domaini740 – 82283ACT 1UniRule annotationAdd
BLAST
Domaini851 – 93181ACT 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 383383UridylyltransferaseAdd
BLAST
Regioni384 – 739356Uridylyl-removingAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation
Contains 2 ACT domains.UniRule annotation
Contains 1 HD domain.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261779.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5ETJ9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDSVTPNSRP ESFPEFDSAG LAAAVDALAA QHSGREDMFR AAVVQLLKAE
60 70 80 90 100
LVKARAVAQA QLLKDRHGRR CAERLCFVQD EIIRILYAAA TQHLYRSQVP
110 120 130 140 150
SGAERMAVVA TGGYGRGLMA PESDIDLLFI LPYKQTAWGE QVAEAILYSL
160 170 180 190 200
WDMGLKVGHA TRSVDESIRQ ARGDMTIRTA ILETRYLAGD RPLYDELVER
210 220 230 240 250
FDTEVVQGTA AEFVAAKLAE REERHRRGGQ SRYLVEPNVK DGKGGLRDLH
260 270 280 290 300
TLFWIAKYVY RVRETAELVE RGVFDAHEYR TFRRCADFLW SVRCNLHFVS
310 320 330 340 350
GRPEERLSFD LQREIAVRLG YTSHPGMQDV ERFMKHYFLV AKEVGNLTAI
360 370 380 390 400
LCAKLEDQQA KPAPVLSRVI SRLKTGNSWR RVPESDDFIV DNNRINLAAP
410 420 430 440 450
DVFKHDPVNL IRIFRLAQKN NLAFHPDAMR AVTRSLNLIN TELRDNPDAN
460 470 480 490 500
RLFMEILTSN DAETVLRRMN ETGVLGHFIR AFGRIVSMMQ FNMYHHYTVD
510 520 530 540 550
EHLIRCIGFL QEIERGGIDE FALASDLMRK IRPEHRAVIY ISVLLHDVAK
560 570 580 590 600
GRPEDHSIAG AKVARRLCPR LGFNNADTEL VAWLIEEHLT MSTVAQSRDL
610 620 630 640 650
SDRRTIEKFA AVVQSVEQMK LLTILTTADI RGVGPGVWNG WKAQLLRTLY
660 670 680 690 700
YETEPVLTGG FSEVNRAKRI TAAQAEFRNA FTDWPEDELN TYIGRHYPAY
710 720 730 740 750
WLKVELPRKI RHARFVRASE DAGHKLAINV GFDPARGVTE LTIFAMDHPW
760 770 780 790 800
LLSIIAGACA SAGANIVDAQ IYTTTDGRAL DTIAISREYE RDEDEGRRAT
810 820 830 840 850
RIGETIEQVL EGKLRLPDAV ARRTTRGKQH KAFSVEPEVS INNQWSELYT
860 870 880 890 900
VIEVSGLDRP GLLYELTTAI SKLNLNIASA HVATFGERAR DVFYVTDLLG
910 920 930
AQINAPTRQA AIKSALLHLL ASDDTAAQPA A
Length:931
Mass (Da):104,895
Last modified:June 12, 2007 - v1
Checksum:i2C910DCB195E85E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000494 Genomic DNA. Translation: ABQ39493.1.
RefSeqiWP_012047384.1. NC_009485.1.
YP_001243399.1. NC_009485.1.

Genome annotation databases

EnsemblBacteriaiABQ39493; ABQ39493; BBta_7648.
GeneIDi5149882.
KEGGibbt:BBta_7648.
PATRICi21215907. VBIBraSp29847_7601.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000494 Genomic DNA. Translation: ABQ39493.1 .
RefSeqi WP_012047384.1. NC_009485.1.
YP_001243399.1. NC_009485.1.

3D structure databases

ProteinModelPortali A5ETJ9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 288000.BBta_7648.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABQ39493 ; ABQ39493 ; BBta_7648 .
GeneIDi 5149882.
KEGGi bbt:BBta_7648.
PATRICi 21215907. VBIBraSp29847_7601.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261779.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci BSP288000:GJBR-7337-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BTAi1 / ATCC BAA-1182.

Entry informationi

Entry nameiGLND_BRASB
AccessioniPrimary (citable) accession number: A5ETJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 12, 2007
Last modified: November 26, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3