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Protein

Hydroxyacylglutathione hydrolase

Gene

gloB

Organism
Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.UniRule annotation

Catalytic activityi

S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per subunit.UniRule annotation

Pathwayi: methylglyoxal degradation

This protein is involved in step 2 of the subpathway that synthesizes (R)-lactate from methylglyoxal.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Hydroxyacylglutathione hydrolase (gloB)
This subpathway is part of the pathway methylglyoxal degradation, which is itself part of Secondary metabolite metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-lactate from methylglyoxal, the pathway methylglyoxal degradation and in Secondary metabolite metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi56Zinc 1UniRule annotation1
Metal bindingi58Zinc 1UniRule annotation1
Metal bindingi60Zinc 2UniRule annotation1
Metal bindingi61Zinc 2UniRule annotation1
Metal bindingi114Zinc 1UniRule annotation1
Metal bindingi133Zinc 1UniRule annotation1
Metal bindingi133Zinc 2UniRule annotation1
Metal bindingi171Zinc 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00619; UER00676.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyacylglutathione hydrolaseUniRule annotation (EC:3.1.2.6UniRule annotation)
Alternative name(s):
Glyoxalase IIUniRule annotation
Short name:
Glx IIUniRule annotation
Gene namesi
Name:gloBUniRule annotation
Ordered Locus Names:BBta_7609
OrganismiBradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182)
Taxonomic identifieri288000 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
Proteomesi
  • UP000000246 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003096291 – 255Hydroxyacylglutathione hydrolaseAdd BLAST255

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi288000.BBta_7609.

Structurei

3D structure databases

ProteinModelPortaliA5ETG1.
SMRiA5ETG1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108RW0. Bacteria.
COG0491. LUCA.
HOGENOMiHOG000058041.
KOiK01069.
OMAiNYIWLLQ.
OrthoDBiPOG091H03YM.

Family and domain databases

CDDicd07723. hydroxyacylglutathione_hydrola. 1 hit.
Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01374. Glyoxalase_2. 1 hit.
InterProiView protein in InterPro
IPR035680. Clx_II_MBL.
IPR032282. HAGH_C.
IPR017782. Hydroxyacylglutathione_Hdrlase.
IPR001279. Metallo-B-lactamas.
IPR036866. Metallo-hydrolase/OxRdtase.
PfamiView protein in Pfam
PF16123. HAGH_C. 1 hit.
PF00753. Lactamase_B. 1 hit.
PIRSFiPIRSF005457. Glx. 1 hit.
SMARTiView protein in SMART
SM00849. Lactamase_B. 1 hit.
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR03413. GSH_gloB. 1 hit.

Sequencei

Sequence statusi: Complete.

A5ETG1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAADIRVFTC LSDNFGYLIH DPATGATASV DAPEAGPIIR QLEAAGWTLT
60 70 80 90 100
DILITHHHHD HVGAVAELKQ KYGCRVVAPH DKTTAIADVD LRVGHGDVIK
110 120 130 140 150
VGELLARVLE TPGHTLDHVS YVFDADKAVF AADTLFSVGC GRVFEGTYPM
160 170 180 190 200
MWDSLLKLRA LPDDFRLYCG HEYTASNVKF ALTVDGDNEA LKARAAEVTR
210 220 230 240 250
LRAANESTIP SLLGDEKQTN VFLRADDPAV AIKLRMKGAT AEQVFGELRE

RKNKS
Length:255
Mass (Da):27,825
Last modified:June 12, 2007 - v1
Checksum:i4E2D61451F7A31B7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000494 Genomic DNA. Translation: ABQ39455.1.
RefSeqiWP_012047346.1. NC_009485.1.

Genome annotation databases

EnsemblBacteriaiABQ39455; ABQ39455; BBta_7609.
KEGGibbt:BBta_7609.

Similar proteinsi

Entry informationi

Entry nameiGLO2_BRASB
AccessioniPrimary (citable) accession number: A5ETG1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: June 12, 2007
Last modified: October 25, 2017
This is version 62 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families