A5ESQ5 (TRUA_BRASB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA pseudouridine synthase A
EC=5.4.99.12

Alternative name(s):
tRNA pseudouridine(38-40) synthase
tRNA pseudouridylate synthase I
tRNA-uridine isomerase I

Gene names

Name:	truA
Ordered Locus Names:	BBta_7335

Organism

Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182) [Complete proteome] [HAMAP]

Taxonomic identifier

288000 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs By similarity. HAMAP-Rule MF_00171
Catalytic activity	tRNA uridine(38-40) = tRNA pseudouridine(38-40). HAMAP-Rule MF_00171
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_00171
Sequence similarities	Belongs to the tRNA pseudouridine synthase TruA family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tRNA pseudouridine synthesis Inferred from electronic annotation. Source: HAMAP
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro pseudouridine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	tRNA pseudouridine synthase A HAMAP-Rule MF_00171		PRO_1000017045
Sites
	Active site	52	1	Nucleophile By similarity
	Binding site	111	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5ESQ5 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 514623A7F177EC83	FASTA	245	27,106
	10 20 30 40 50 60 MPRYKLTIEY DGTPFCGWQL QETLPSVQGA LEAAVQATCG VPTRVHGSGR TDAGVHATGQ 70 80 90 100 110 120 VAHCDIAKDF RPDKLRDALN AHLRPNPVAV LEAEIVSDTF EARFSARKRH YRYRIVNRRS 130 140 150 160 170 180 NLALEVGRVW RVPQRLDSDA MHAAAQRLIG RHDFTTFRDT ECQAKSPEKT LDQLDVVRDG 190 200 210 220 230 240 DAVTILTSAR SYLHSQVRSM VGSLVWVGQG RWSADDLAAA LAARNRAACG VVAPPEGLYL VKVDY

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BTAi1 / ATCC BAA-1182.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000494 Genomic DNA. Translation: ABQ39199.1.
RefSeq	YP_001243105.1. NC_009485.1.
3D structure databases
ProteinModelPortal	A5ESQ5.
ModBase	Search...
Protein-protein interaction databases
STRING	288000.BBta_7335.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABQ39199; ABQ39199; BBta_7335.
GeneID	5149518.
KEGG	bbt:BBta_7335.
PATRIC	21215295. VBIBraSp29847_7296.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0101.
HOGENOM	HOG000248672.
KO	K06173.
OMA	RHYRYRI.
ProtClustDB	PRK00021.
Enzyme and pathway databases
BioCyc	BSP288000:GJBR-7039-MONOMER.
Family and domain databases
Gene3D	3.30.70.580. 1 hit. 3.30.70.660. 1 hit.
HAMAP	MF_00171. TruA.
InterPro	IPR020103. PsdUridine_synth_cat_dom. IPR001406. PsdUridine_synth_TruA. IPR020097. PsdUridine_synth_TruA_a/b_dom. IPR020095. PsdUridine_synth_TruA_C. IPR020094. PsdUridine_synth_TruA_N. [Graphical view]
PANTHER	PTHR11142. PTHR11142. 1 hit.
Pfam	PF01416. PseudoU_synth_1. 2 hits. [Graphical view]
PIRSF	PIRSF001430. tRNA_psdUrid_synth. 1 hit.
SUPFAM	SSF55120. PsdUridine_synth_cat_dom. 1 hit.
TIGRFAMs	TIGR00071. hisT_truA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRUA_BRASB

Accession

Primary (citable) accession number: A5ESQ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 12, 2007
Last modified:	May 1, 2013
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program