A5ES06 (GLPK_BRASB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol kinase
EC=2.7.1.30

Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	BBta_7067

Organism

Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182) [Complete proteome] [HAMAP]

Taxonomic identifier

288000 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 500	500	Glycerol kinase HAMAP MF_00186		PRO_1000020706
Regions
	Nucleotide binding	411 – 415	5	ATP By similarity
Sites
	Binding site	11	1	Substrate By similarity
	Binding site	15	1	ATP By similarity
	Binding site	81	1	Substrate By similarity
	Binding site	133	1	Substrate By similarity
	Binding site	242	1	Substrate By similarity
	Binding site	264	1	ATP By similarity
	Binding site	307	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5ES06 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: A9E14A2A5436CADC	FASTA	500	54,125
	10 20 30 40 50 60 MSFVLAIDQG TTSSRAIVFR SDISIAAVAQ QEFPQHFPAS GWVEHEPEDI WTSTVMTCRD 70 80 90 100 110 120 ALDKAGIKAK DIAAIGITNQ RETTVVWDRA TGQAVHRAIV WQDRRTADIC AKLKADGYEP 130 140 150 160 170 180 DVSAKTGLII DPYFSGTKVA WILDHVPGAR ERAERGELLF GTVDCYLLWR LTGGRVHATD 190 200 210 220 230 240 ATNASRTLLF NIHTGQWDDT LLKLLRVPRS MLPEVKDSSA EFGTTTPDLF GGPIKVSGIA 250 260 270 280 290 300 GDQQAATIGQ ACFTPGMMKS TYGTGCFALL NTGATPVKSN NKLLTTIAYQ LGGVRTYALE 310 320 330 340 350 360 GSIFVAGSAV QWLRDGLGII KHAAETGPLA DKSDSMQSVY LVPAFVGLGA PYWNPRVRGA 370 380 390 400 410 420 LFGLTRNTGP AELAHATLES VCYQTYDLWA AMRADWPDAS AATIVLRVDG GMTASDWTMQ 430 440 450 460 470 480 RLADLLDAPV DRPMIQETTA LGAAYLAGLN AGVYPEPEKF ADNWRLEHRF KPAMSAATRQ 490 500 RKLAGWARAV KGVLASDEGE

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BTAi1 / ATCC BAA-1182.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000494 Genomic DNA. Translation: ABQ38950.1.
RefSeq	YP_001242856.1. NC_009485.1.
3D structure databases
ProteinModelPortal	A5ES06.
ModBase	Search...
Protein-protein interaction databases
STRING	A5ES06.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5151352.
GenomeReviews	Gene locus BBta_7067 in contig CP000494_GR.
KEGG	bbt:BBta_7067.
PATRIC	21214791. VBIBraSp29847_7045.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0554.
HOGENOM	HBG511469.
OMA	CYQTRDL.
ProtClustDB	CLSK818559.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
KO	K00864.
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. Glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_BRASB

Accession

Primary (citable) accession number: A5ES06

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 12, 2007
Last modified:	December 14, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program