Tryptophan 2,3-dioxygenase - Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Tryptophan 2,3-dioxygenase
Short name=TDO
EC=1.13.11.11

Alternative name(s):
Tryptamin 2,3-dioxygenase
Tryptophan oxygenase
Short name=TO
Short name=TRPO
Tryptophan pyrrolase
Tryptophanase

Gene names

Name:	kynA
Ordered Locus Names:	BBta_6511

Organism

Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182) [Complete proteome] [HAMAP]

Taxonomic identifier

288000 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	279 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O₂ = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB tryptophan catabolic process to kynurenine Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	heme binding Inferred from sequence or structural similarity. Source: UniProtKB tryptophan 2,3-dioxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 279

279

Tryptophan 2,3-dioxygenase

PRO_0000360094

Regions

Region

23 – 27

5

Substrate binding By similarity

Region

48 – 52

5

Substrate binding By similarity

Sites

Metal binding

237

1

Iron (heme axial ligand) By similarity

Binding site

110

1

Substrate By similarity

Binding site

114

1

Substrate By similarity

Binding site

121

1

Heme By similarity

Binding site

251

1

Substrate By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

A5EQH6 [UniParc].

Last modified June 12, 2007. Version 1.
Checksum: B36E6C4E029ACDE2
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FASTA

279

32,154

        10         20         30         40         50         60 
MSNAPYDPAT EGARMNFKGR MSYGDYLMLD RLLDAQAPLS SAHDELLFII QHQTSELWMK 

        70         80         90        100        110        120 
LAIHEIKAAM AAIAKDDVQP AFKMLARVSR IFEQLNGAWD VLRTMTPSEY TLFRDKLGES 

       130        140        150        160        170        180 
SGFQSFQYRS IEFLLGNRNL AMLRPHAHHP ELTAELEAIL AKPSLYDEAL KLLARRGFFI 

       190        200        210        220        230        240 
GADGQRTDWR GTRSESPEVA AAWTSVYRDP QRHWELYELA EKLVDFEDYF RRWRFNHVTT 

       250        260        270 
VERIIGFKTG TGGTSGVNYL RKMLEIVLFP ELWKLRTGL

« Hide

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia."
Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z.

Sadowsky M.
Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BTAi1 / ATCC BAA-1182.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000494 Genomic DNA. Translation: ABQ38420.1.
RefSeq	YP_001242326.1. NC_009485.1.
3D structure databases
ProteinModelPortal	A5EQH6.
SMR	A5EQH6. Positions 21-279.
ModBase	Search...
Protein-protein interaction databases
STRING	A5EQH6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5155858.
GenomeReviews	Gene locus BBta_6511 in contig CP000494_GR.
KEGG	bbt:BBta_6511.
PATRIC	21213705. VBIBraSp29847_6506.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3483.
HOGENOM	HBG647485.
OMA	QYREIEF.
ProtClustDB	CLSK783013.
Family and domain databases
InterPro	IPR017485. Trp_2-3-dOase_bac. IPR004981. Trp_2_3_dOase. [Graphical view]
KO	K00453.
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 2 hits. [Graphical view]
TIGRFAMs	TIGR03036. Trp_2_3_diox. 1 hit.
ProtoNet	Search...

Entry information

Entry name

T23O_BRASB

Accession

Primary (citable) accession number: A5EQH6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	June 12, 2007
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents