A5EQB8 (HUTI_BRASB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Imidazolonepropionase
EC=3.5.2.7

Alternative name(s):
Imidazolone-5-propionate hydrolase

Gene names

Name:	hutI
Ordered Locus Names:	BBta_6452

Organism

Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182) [Complete proteome] [HAMAP]

Taxonomic identifier

288000 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	404 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+. HAMAP MF_00372
Cofactor	Binds 1 zinc or iron ion per subunit By similarity. HAMAP MF_00372
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 3/3. HAMAP MF_00372
Subcellular location	Cytoplasm Potential HAMAP MF_00372.
Sequence similarities	Belongs to the HutI family.

Ontologies

Keywords
Biological process	Histidine metabolism
Cellular component	Cytoplasm
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	histidine catabolic process to glutamate and formamide Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	imidazolonepropionase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 404	404	Imidazolonepropionase HAMAP MF_00372		PRO_0000306441
Sites
	Metal binding	73	1	Zinc or iron By similarity
	Metal binding	75	1	Zinc or iron By similarity
	Metal binding	243	1	Zinc or iron By similarity
	Metal binding	318	1	Zinc or iron By similarity
	Binding site	82	1	Substrate By similarity
	Binding site	95	1	Substrate By similarity
	Binding site	145	1	Substrate By similarity
	Binding site	178	1	Substrate By similarity
	Binding site	246	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5EQB8 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: B4CE212C4A554FF3	FASTA	404	43,037
	10 20 30 40 50 60 MAERFDRIWH NARLATLREG QPGLGVIEQG VVAARDGRIA FAGPRSDFAA DGDAPERIDC 70 80 90 100 110 120 AGRWITPGLV DCHTHLVYGG DRAHEFELRL AGASYEEIAR AGGGIVSTVA ATRAADENEL 130 140 150 160 170 180 VTGALPRLDA LLAEGVTTIE IKSGYGLETA TELRQLSAAR ALGVRRAVSV RTSFLGAHAL 190 200 210 220 230 240 PVEADGDKER YIDRVCDEML PAVAQSGLAD AVDAFMENIA FSAAQTSRVF AAAKALGLPV 250 260 270 280 290 300 KLHADQLSNL GGAALAAEFG ALSADHLEHT DEAGAAAMAR AETVAVLLPG AFYFIRETQK 310 320 330 340 350 360 PPVELFRKHG VKLALATDCN PGSSPLTSLL LTMNMAATLF RMTVDECLAG VTREGARALG 370 380 390 400 VLAETGTLDA GKWCDLAIWD INRPAELVYR MGFNPLHQRV WRGR

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BTAi1 / ATCC BAA-1182.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000494 Genomic DNA. Translation: ABQ38362.1.
RefSeq	YP_001242268.1. NC_009485.1.
3D structure databases
ProteinModelPortal	A5EQB8.
SMR	A5EQB8. Positions 8-404.
ModBase	Search...
Protein-protein interaction databases
STRING	A5EQB8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5154929.
GenomeReviews	Gene locus BBta_6452 in contig CP000494_GR.
KEGG	bbt:BBta_6452.
PATRIC	21213587. VBIBraSp29847_6447.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1228.
HOGENOM	HBG686142.
OMA	MNMACTL.
ProtClustDB	PRK09356.
Family and domain databases
HAMAP	MF_00372. HutI. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR005920. HutI. IPR011059. Metal-dep_hydrolase_composite. [Graphical view]
KO	K01468.
PANTHER	PTHR22642. PTHR22642. 1 hit.
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
SUPFAM	SSF51338. Metalo_hydrolase. 1 hit.
TIGRFAMs	TIGR01224. HutI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HUTI_BRASB

Accession

Primary (citable) accession number: A5EQB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	June 12, 2007
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program