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Protein

Light-independent protochlorophyllide reductase subunit N

Gene

bchN

Organism
Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Component of the dark-operative protochlorophyllide reductase (DPOR) that uses Mg-ATP and reduced ferredoxin to reduce ring D of protochlorophyllide (Pchlide) to form chlorophyllide a (Chlide). This reaction is light-independent. The NB-protein (BchN-BchB) is the catalytic component of the complex.UniRule annotation

Catalytic activityi

Protochlorophyllide a + reduced ferredoxin + 2 ATP + 2 H2O = chlorophyllide a + oxidized ferredoxin + 2 ADP + 2 phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per heterodimer. The cluster is bound at the heterodimer interface by residues from both subunits.UniRule annotation

Pathwayi: bacteriochlorophyll biosynthesis (light-independent)

This protein is involved in the pathway bacteriochlorophyll biosynthesis (light-independent), which is part of Porphyrin-containing compound metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway bacteriochlorophyll biosynthesis (light-independent) and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi30 – 301Iron-sulfur (4Fe-4S); shared with heterodimeric partnerUniRule annotation
Metal bindingi55 – 551Iron-sulfur (4Fe-4S); shared with heterodimeric partnerUniRule annotation
Metal bindingi116 – 1161Iron-sulfur (4Fe-4S); shared with heterodimeric partnerUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Bacteriochlorophyll biosynthesis, Chlorophyll biosynthesis, Photosynthesis

Keywords - Ligandi

4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSP288000:GJBR-6152-MONOMER.
UniPathwayiUPA00671.

Names & Taxonomyi

Protein namesi
Recommended name:
Light-independent protochlorophyllide reductase subunit NUniRule annotation (EC:1.3.7.7UniRule annotation)
Short name:
DPOR subunit NUniRule annotation
Short name:
LI-POR subunit NUniRule annotation
Gene namesi
Name:bchNUniRule annotation
Ordered Locus Names:BBta_6417
OrganismiBradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182)
Taxonomic identifieri288000 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
Proteomesi
  • UP000000246 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 428428Light-independent protochlorophyllide reductase subunit NPRO_0000324000Add
BLAST

Interactioni

Subunit structurei

Protochlorophyllide reductase is composed of three subunits; BchL, BchN and BchB. Forms a heterotetramer of two BchB and two BchN subunits.UniRule annotation

Protein-protein interaction databases

STRINGi288000.BBta_6417.

Family & Domainsi

Sequence similaritiesi

Belongs to the BchN/ChlN family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C78. Bacteria.
COG2710. LUCA.
HOGENOMiHOG000265994.
KOiK04038.
OMAiIFAEPRF.
OrthoDBiEOG6PZX7T.

Family and domain databases

HAMAPiMF_00352. ChlN_BchN.
InterProiIPR000510. Nase/OxRdtase_comp1.
IPR005970. Protochl_reductN.
[Graphical view]
PfamiPF00148. Oxidored_nitro. 1 hit.
[Graphical view]
PIRSFiPIRSF000162. P_chlorophyll_rd. 1 hit.
TIGRFAMsiTIGR01279. DPOR_bchN. 1 hit.

Sequencei

Sequence statusi: Complete.

A5EQ83-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNAHAQACAV TSAASPDGVL RERGQREVFC GLTGIVWLHR KIQDAFFLVV
60 70 80 90 100
GSRTCAHLIQ SAAGVMIFAE PRFATAIMEE RDLAGLVDAN DELDRIVTQL
110 120 130 140 150
LARRPEIKLL FLVGSCPSEV IKLDLSRAAL RLSQRFSPGV RVLNYSGSGI
160 170 180 190 200
ETTFTQGEDA CLASLVPVLP AADRSARPSL LVVGALADVV EDQFKRTFAA
210 220 230 240 250
LGIDQVAFLP PRRSSELPAI GPETRVLLAQ PFLGDTARAL EERGCRRIAA
260 270 280 290 300
PFPLGGEGTA LWLAAAADAF GVSHAHVERT IAPLKARAER ALARYRAQLA
310 320 330 340 350
GKRVFLFPDS QIEIPLARFL SREIGMELVE VGTPYLHRDH LASELALLPG
360 370 380 390 400
GTQLSEGQDV ERQLDRCRDA RPDLVVCGLG LANPLEAEGL TTKWSIELIF
410 420
TPIQGFEQAG DLAELFARPL LRQTRLAV
Length:428
Mass (Da):46,434
Last modified:June 12, 2007 - v1
Checksum:i862B4901E0761FB1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000494 Genomic DNA. Translation: ABQ38327.1.
RefSeqiWP_012046268.1. NC_009485.1.

Genome annotation databases

EnsemblBacteriaiABQ38327; ABQ38327; BBta_6417.
KEGGibbt:BBta_6417.
PATRICi21213517. VBIBraSp29847_6412.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000494 Genomic DNA. Translation: ABQ38327.1.
RefSeqiWP_012046268.1. NC_009485.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi288000.BBta_6417.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ38327; ABQ38327; BBta_6417.
KEGGibbt:BBta_6417.
PATRICi21213517. VBIBraSp29847_6412.

Phylogenomic databases

eggNOGiENOG4105C78. Bacteria.
COG2710. LUCA.
HOGENOMiHOG000265994.
KOiK04038.
OMAiIFAEPRF.
OrthoDBiEOG6PZX7T.

Enzyme and pathway databases

UniPathwayiUPA00671.
BioCyciBSP288000:GJBR-6152-MONOMER.

Family and domain databases

HAMAPiMF_00352. ChlN_BchN.
InterProiIPR000510. Nase/OxRdtase_comp1.
IPR005970. Protochl_reductN.
[Graphical view]
PfamiPF00148. Oxidored_nitro. 1 hit.
[Graphical view]
PIRSFiPIRSF000162. P_chlorophyll_rd. 1 hit.
TIGRFAMsiTIGR01279. DPOR_bchN. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BTAi1 / ATCC BAA-1182.

Entry informationi

Entry nameiBCHN_BRASB
AccessioniPrimary (citable) accession number: A5EQ83
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: June 12, 2007
Last modified: November 11, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.