ID   ACSF_BRASB              Reviewed;         353 AA.
AC   A5EQ73;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   12-JUN-2007, sequence version 1.
DT   05-MAY-2009, entry version 13.
DE   RecName: Full=Aerobic magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase;
DE            Short=Aerobic Mg-protoporphyrin IX monomethyl ester oxidative cyclase;
DE            EC=1.14.13.81;
GN   Name=acsF; OrderedLocusNames=BBta_6407;
OS   Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium.
OX   NCBI_TaxID=288000;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=17540897; DOI=10.1126/science.1139548;
RA   Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C.,
RA   Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L.,
RA   Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S.,
RA   Rouy Z., Mangenot S., Segurens B., Dossat C., Franck W.L.,
RA   Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P.,
RA   Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A.,
RA   Medigue C., Sadowsky M.;
RT   "Legumes symbioses: absence of nod genes in photosynthetic
RT   bradyrhizobia.";
RL   Science 316:1307-1312(2007).
CC   -!- FUNCTION: Catalyzes the formation of the isocyclic ring in
CC       chlorophyll biosynthesis. Mediates the cyclase reaction, which
CC       results in the formation of divinylprotochlorophyllide (Pchlide)
CC       characteristic of all chlorophylls from magnesium-protoporphyrin
CC       IX 13-monomethyl ester (MgPMME) (By similarity).
CC   -!- CATALYTIC ACTIVITY: Magnesium-protoporphyrin IX 13-monomethyl
CC       ester + NADPH + O(2) = 13(1)-hydroxy-magnesium-protoporphyrin IX
CC       13-monomethyl ester + NADP(+) + H(2)O.
CC   -!- CATALYTIC ACTIVITY: 13(1)-hydroxy-magnesium-protoporphyrin IX 13-
CC       monomethyl ester + NADPH + O(2) = 13(1)-oxo-magnesium-
CC       protoporphyrin IX 13-monomethyl ester + NADP(+) + 2 H(2)O.
CC   -!- CATALYTIC ACTIVITY: 13(1)-oxo-magnesium-protoporphyrin IX 13-
CC       monomethyl ester + NADPH + O(2) = divinylprotochlorophyllide +
CC       NADP(+) + 2 H(2)O.
CC   -!- COFACTOR: Iron (By similarity).
CC   -!- PATHWAY: Porphyrin biosynthesis; bacteriochlorophyll biosynthesis
CC       (light-independent).
CC   -!- SIMILARITY: Belongs to the acsF family.
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DR   EMBL; CP000494; ABQ38317.1; -; Genomic_DNA.
DR   RefSeq; YP_001242223.1; -.
DR   GeneID; 5154687; -.
DR   GenomeReviews; CP000494_GR; BBta_6407.
DR   KEGG; bbt:BBta_6407; -.
DR   OMA; A5EQ73; TAEFSGC.
DR   GO; GO:0005506; F:iron ion binding; IEA:HAMAP.
DR   GO; GO:0048529; F:magnesium-protoporphyrin IX monomethyl este...; IEA:HAMAP.
DR   GO; GO:0030494; P:bacteriochlorophyll biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0015979; P:photosynthesis; IEA:HAMAP.
DR   HAMAP; MF_01840; -; 1.
DR   InterPro; IPR008434; AcsF.
DR   InterPro; IPR003251; Rubrerythrin.
DR   Pfam; PF02915; Rubrerythrin; 1.
DR   TIGRFAMs; TIGR02029; AcsF; 1.
PE   3: Inferred from homology;
KW   Bacteriochlorophyll biosynthesis; Chlorophyll biosynthesis;
KW   Complete proteome; Iron; Metal-binding; NADP; Oxidoreductase;
KW   Photosynthesis.
FT   CHAIN         1    353       Aerobic magnesium-protoporphyrin IX
FT                                monomethyl ester [oxidative] cyclase.
FT                                /FTId=PRO_1000070543.
SQ   SEQUENCE   353 AA;  41246 MW;  EFDCC63CEEA9326B CRC64;
     MIAMEGGAGN ISTKMALEDT ILTPRFYTTD FAAMDRLNVD LVRREWDAVM AELRADHNRK
     HFVRTPEFEK DLNELPEALR LEFKDFLVSS LTAEFSGCVL YAEIKKRITN PDIRELFSYM
     SRDEARHAGF INEILKDHGI GVDLGFLTKV KKYTYFKPKF IFYATYLSEK IGYARYITIY
     RQMERHPERQ FHPIFKWFER WCNDEFRHGE AFALLMRADP KLLSGLNKLW IKFFLLAVFA
     TMHVRDHMRP AFYEALGMPV DEYDMRVFRI TSEISRQVFP VMLDIDNPRF WAGLNQLRKV
     SEAIADAKAQ GGVMGAIKRA VLPLKAALTF GRLYMLPAKS NELPREIRLQ PAW
//