Reviewed, UniProtKB/Swiss-Prot A5EQ67 (F16A2_BRASB)

Names and origin

Protein names

Recommended name:
    Fructose-1,6-bisphosphatase class 1 2
      Short name=FBPase class 1 2
    EC=3.1.3.11
Alternative name(s):
    D-fructose-1,6-bisphosphate 1-phosphohydrolase class 1 2

Gene names

Name:	fbp2
Ordered Locus Names:	BBta_6401

Organism

Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182) [Complete proteome] [HAMAP]

Taxonomic identifier

288000 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate. HAMAP MF_01855
Cofactor	Binds 2 magnesium ions per subunit By similarity.
Pathway	Carbohydrate biosynthesis; Calvin cycle. HAMAP MF_01855
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the FBPase class 1 family.

Ontologies

Keywords
Biological process	Calvin cycle Carbohydrate metabolism
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	fructose 1,6-bisphosphate 1-phosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	Fructose-1,6-bisphosphatase class 1 2 HAMAP MF_01855		PRO_0000364474
Regions
	Region	114 – 117	4	Substrate binding By similarity
Sites
	Metal binding	92	1	Magnesium 1 By similarity
	Metal binding	111	1	Magnesium 1 By similarity
	Metal binding	111	1	Magnesium 2 By similarity
	Metal binding	113	1	Magnesium 1; via carbonyl oxygen By similarity
	Metal binding	114	1	Magnesium 2 By similarity
	Metal binding	274	1	Magnesium 2 By similarity
	Binding site	202	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5EQ67-1 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 3CF38AC1700237E1	FASTA	347	37,935
	10 20 30 40 50 60 MAREWPMEQS PTLQAHLALQ NADADCAAVI EALADAAREL ARQIAIAPLA GFDEGAATVN 70 80 90 100 110 120 ADGDVQKALD IVADNLMRDA LRKAPVAGIL SEEVDRPETV NAAAPLCVAI DPLDGSSNLQ 130 140 150 160 170 180 NNISVGTIFS IRPRGRDVLS SFFEPGTAQR AAGFFVYGPQ TCLVLAIDHR VDLYVLHPTL 190 200 210 220 230 240 REFVLARSGL RIPQDTPEFA INASNRRHWS GTVRNYVDEC LAGAAGPRGR DFNMRWIASL 250 260 270 280 290 300 VAEAYRILMR GGVFLYPADS RPGYREGRLR LVYEAHPMAL IMEWAGGSAS SGRSRILELS 310 320 330 340 ARSPHQRAPL IMGDVRLVRD VDQLHEGVEP LFETSDAPLF ARRGLFR

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000494 Genomic DNA. Translation: ABQ38311.1.
RefSeq	YP_001242217.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A5EQ67.
Genome annotation databases
GeneID	5153621.
GenomeReviews	Gene locus BBta_6401 in contig CP000494_GR.
KEGG	bbt:BBta_6401.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	QACKTIS.
Family and domain databases
HAMAP	MF_01855. [Tree]
InterPro	IPR000146. Fructose_bisphosphatase. IPR020548. Fructose_bisphosphatase_AS. [Graphical view]
PANTHER	PTHR11556. In_FB_phphtase. 1 hit.
Pfam	PF00316. FBPase. 1 hit. [Graphical view]
PRINTS	PR00115. F16BPHPHTASE.
ProDom	PD001491. In_FB_phphtase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00124. FBPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

F16A2_BRASB

Accession

Primary (citable) accession number: A5EQ67

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	June 12, 2007
Last modified:	November 3, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)