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A5EPZ7 (GLGB_BRASB) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:BBta_6323
OrganismBradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182) [Complete proteome] [HAMAP]
Taxonomic identifier288000 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium

Protein attributes

Sequence length712 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7127121,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_1000061987

Sites

Active site3971Nucleophile By similarity
Active site4501Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
A5EPZ7 [UniParc].

Last modified June 12, 2007. Version 1.
Checksum: 6427EB512CB6A65C

FASTA71281,072
        10         20         30         40         50         60 
MTHLSPEAFA IIEGRHADPF RYLGQHDVDG RTVIRAFLPE ASRVEAVGDN GEVAPLARIH 

        70         80         90        100        110        120 
DAGLFTGTMS GPQRYRLRAT FGDHVTDLED PYRFLPILTD FDLHLLGEGN HERLYDKLGA 

       130        140        150        160        170        180 
HPMVLDGVDG VGFVVLAPNA RRVSVVGDFN FWNARRHPMR VRGNGYWELF IPRAKAGDHY 

       190        200        210        220        230        240 
KFDIIGPQGE HLPLKSDPMA FAAEMRPKTA SIVVDETRLP RPRPAPSDIN KLDRPVSIYE 

       250        260        270        280        290        300 
VHLGSWRRKD NNQWLTYREL AEQLPAYARD MGFTHIEFLP VSEHPFDGSW GYQPTGLFAP 

       310        320        330        340        350        360 
TSRFGSPEDF CALVDACHRE GLAVWLDWVP GHFPDDPHGL GHFDGTALYE HANPMQGRHL 

       370        380        390        400        410        420 
DWGTLIYNYG RTEVANFLRS NALFWLERYG IDGLRVDAVA SMLYLDYSRP SGGWIPNKYG 

       430        440        450        460        470        480 
GRENLEAIEF LRRTNIEVFG HFPQATTAAE ESTAWPQVSR PVDIGGLGFG YKWNMGWMHD 

       490        500        510        520        530        540 
TLRYVGKDPI HRKYHHGEIL FGLHYAFSEN FILPLSHDEV VHGKRSILGR MPGDDWQRFA 

       550        560        570        580        590        600 
NLRAYYSFMF GHPGKKLLFM GCEIAQEREW NHDSSLDWHL LGDAKYAGIQ ALIRDLNHLY 

       610        620        630        640        650        660 
RNQPALHERD CEPEGFDWLI TEDADRNVFA WVRKGFDERA RCVVVVNFSP NVYYNYRVRV 

       670        680        690        700        710 
PLGGTWREVF NSDSSHYGGS NVGNVGQVHA SEDQQLNLIL PPMAAVFLVP EA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000494 Genomic DNA. Translation: ABQ38241.1.
RefSeqYP_001242147.1. NC_009485.1.

3D structure databases

ProteinModelPortalA5EPZ7.
SMRA5EPZ7. Positions 110-712.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING288000.BBta_6323.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABQ38241; ABQ38241; BBta_6323.
GeneID5152404.
KEGGbbt:BBta_6323.
PATRIC21213337. VBIBraSp29847_6323.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAAKLLFMG.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycBSP288000:GJBR-6063-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_BRASB
AccessionPrimary (citable) accession number: A5EPZ7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: June 12, 2007
Last modified: June 11, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries