Reviewed, UniProtKB/Swiss-Prot A5EPK1 (MURC_BRASB)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramate--L-alanine ligase
    EC=6.3.2.8
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	BBta_6171

Organism

Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182) [Complete proteome] [HAMAP]

Taxonomic identifier

288000 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	467 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 467	467	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_1000004314
Regions
	Nucleotide binding	114 – 120	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A5EPK1-1 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 5F80B29F634098A9	FASTA	467	49,857
	10 20 30 40 50 60 MRLPREIGPI HFVGIGGIGM SGIAEVLCNL GYTVQGSDAS DNANVARLRE KGITVSVGHK 70 80 90 100 110 120 AENVAGADVV VVSTAIKRDN PELMTARAQR IPVVRRAEML AELMRLKSCV AIAGTHGKTT 130 140 150 160 170 180 TTSMVAALLD AGGIDPTVIN GGIINAYGTN ARLGAGDWMV VEADESDGTF LKLPADVAIV 190 200 210 220 230 240 TNVDPEHLDH FKTFDAVQDA FRAFVENVPF YGFAVMCIDH PVVQALVGKI EDRRIITYGV 250 260 270 280 290 300 NPQADVRLVD LSPANGSSRF KVVFRDRKSG ATHEISDITL PMPGRHNASN ATAAIAVAHE 310 320 330 340 350 360 LGISDEAIRK AIAGFGGVKR RFTRTGEWNG VTIIDDYGHH PVEIAAVLKA ARESTSGKVI 370 380 390 400 410 420 AVVQPHRYTR LQSLFEEFCT CFNDADAVIV ADVYPAGEAP IEGIDRDHFV LGLRAHGHRD 430 440 450 460 VLPLPQAADL AGIVKRLAKS GDLVVCLGAG NITQWAYALP NELKALG

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000494 Genomic DNA. Translation: ABQ38095.1.
RefSeq	YP_001242001.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A5EPK1.
Genome annotation databases
GeneID	5152035.
GenomeReviews	Gene locus BBta_6171 in contig CP000494_GR.
KEGG	bbt:BBta_6171.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	EWMVVEA.
Family and domain databases
HAMAP	MF_00046. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01082. murC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_BRASB

Accession

Primary (citable) accession number: A5EPK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 12, 2007
Last modified:	November 3, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)