ID   A5ENT6_BRASB            Unreviewed;       250 AA.
AC   A5ENT6;
DT   12-JUN-2007, integrated into UniProtKB/TrEMBL.
DT   12-JUN-2007, sequence version 1.
DT   27-MAR-2024, entry version 66.
DE   RecName: Full=carboxymethylenebutenolidase {ECO:0000256|ARBA:ARBA00044062};
DE            EC=3.1.1.45 {ECO:0000256|ARBA:ARBA00044062};
GN   OrderedLocusNames=BBta_5888 {ECO:0000313|EMBL:ABQ37830.1};
OS   Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182).
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC   Nitrobacteraceae; Bradyrhizobium.
OX   NCBI_TaxID=288000 {ECO:0000313|EMBL:ABQ37830.1, ECO:0000313|Proteomes:UP000000246};
RN   [1] {ECO:0000313|EMBL:ABQ37830.1, ECO:0000313|Proteomes:UP000000246}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=BTAi1 / ATCC BAA-1182 {ECO:0000313|Proteomes:UP000000246};
RX   PubMed=17540897; DOI=10.1126/science.1139548;
RA   Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.C.,
RA   Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L.,
RA   Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z.,
RA   Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.S., Saunders E.,
RA   Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G.,
RA   Emerich D., Vermeglio A., Medigue C., Sadowsky M.;
RT   "Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia.";
RL   Science 316:1307-1312(2007).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-(5-oxo-2,5-dihydrofuran-2-ylidene)acetate + H2O = 4-oxohex-
CC         2-enedioate + H(+); Xref=Rhea:RHEA:12372, ChEBI:CHEBI:12040,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57263; EC=3.1.1.45;
CC         Evidence={ECO:0000256|ARBA:ARBA00043789};
CC   -!- SIMILARITY: Belongs to the dienelactone hydrolase family.
CC       {ECO:0000256|ARBA:ARBA00008456}.
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DR   EMBL; CP000494; ABQ37830.1; -; Genomic_DNA.
DR   RefSeq; WP_012045787.1; NC_009485.1.
DR   AlphaFoldDB; A5ENT6; -.
DR   STRING; 288000.BBta_5888; -.
DR   ESTHER; brasb-a5ent6; Dienelactone_hydrolase.
DR   KEGG; bbt:BBta_5888; -.
DR   eggNOG; COG0412; Bacteria.
DR   HOGENOM; CLU_054590_7_3_5; -.
DR   OrthoDB; 9771666at2; -.
DR   Proteomes; UP000000246; Chromosome.
DR   GO; GO:0008806; F:carboxymethylenebutenolidase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR002925; Dienelactn_hydro.
DR   PANTHER; PTHR46623; CARBOXYMETHYLENEBUTENOLIDASE-RELATED; 1.
DR   PANTHER; PTHR46623:SF11; CARBOXYMETHYLENEBUTENOLIDASE-RELATED; 1.
DR   Pfam; PF01738; DLH; 1.
DR   SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
PE   3: Inferred from homology;
KW   Hydrolase {ECO:0000313|EMBL:ABQ37830.1}.
FT   DOMAIN          17..213
FT                   /note="Dienelactone hydrolase"
FT                   /evidence="ECO:0000259|Pfam:PF01738"
SQ   SEQUENCE   250 AA;  26215 MW;  0125EE417B513E19 CRC64;
     MPTESTIKLT AGDGHTFQAF RADPTETPKG AIVVLPDDND GPRRLRKVAD TFTASGYVVI
     TPELSAMAKP APDSEPSAAV PDPAERLLAE IQSTVDSVKD CGKVAVIGYG AGGGLAYDAA
     NRVSGLACAV SYYATGVVEA AGAKRKVPTL LHFGEADAVV PFPAVSMFRA HHPEVSAFSY
     PHAAHEFDGD GDTYDEAAAA LAQQRTLAWI SQYVVGQPPI SLKNAGAYAL AKVDKKKKKK
     ADDDMGPPME
//