Reviewed, UniProtKB/Swiss-Prot A5ENA7 (SAHH_BRASB)

Names and origin

Protein names

Recommended name:
    Adenosylhomocysteinase
    EC=3.3.1.1
Alternative name(s):
    S-adenosyl-L-homocysteine hydrolase
      Short name=AdoHcyase

Gene names

Name:	ahcY
Ordered Locus Names:	BBta_5698

Organism

Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182) [Complete proteome] [HAMAP]

Taxonomic identifier

288000 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	473 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine. HAMAP MF_00563
Cofactor	Binds 1 NAD per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. HAMAP MF_00563
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the adenosylhomocysteinase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	adenosylhomocysteinase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 473	473	Adenosylhomocysteinase HAMAP MF_00563		PRO_1000024710
Regions
	Region	224 – 389	166	NAD binding By similarity
Sites
	Binding site	60	1	Substrate By similarity
	Binding site	135	1	Substrate By similarity
	Binding site	197	1	Substrate By similarity
	Binding site	227	1	Substrate By similarity
	Binding site	231	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5ENA7-1 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 17DACFD90AEC2D44	FASTA	473	51,751
	10 20 30 40 50 60 MTAKPGFNDY IVKDISLAEF GRKEISLAET EMPGLMATRE EYGPKQPLKG ARIAGSLHMT 70 80 90 100 110 120 IQTAVLIETL AALGADIRWV SCNIYSTQDH AAAAIAAAGI PVFAVKGETL ADYWDYTAKL 130 140 150 160 170 180 FDWHGGGHPN MILDDGGDAT MYVHLGLRAE NGDTAFLDKP ASEEEEVFFA LLKKQLKEKP 190 200 210 220 230 240 KGYFAAIAAS IKGVSEETTT GVHRLYDMQK AGTLLWPAIN VNDSVTKSKF DNLYGCRESL 250 260 270 280 290 300 VDGIRRGTDV MMSGKVAMVA GFGDVGKGSA ASLRQAGCRV LVSEIDPICA LQAAMEGYEV 310 320 330 340 350 360 VTMEDAAPRA DIFVTATGNK DIITIEHMRA MKDRAIVCNI GHFDNEIQVA SLKNLKWTNI 370 380 390 400 410 420 KPQVDEITFP DGKRMILLSE GRLVNLGNAM GHPSFVMSAS FTNQTLAQIE LYANNKDGKY 430 440 450 460 470 KKEVYVLPKS LDEKVARLHL AKIGVKLTEL RPDQAAYIGV KQEGPFKSDH YRY

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000494 Genomic DNA. Translation: ABQ37651.1.
RefSeq	YP_001241557.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A5ENA7.
Genome annotation databases
GeneID	5149355.
GenomeReviews	Gene locus BBta_5698 in contig CP000494_GR.
KEGG	bbt:BBta_5698.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	HMRAMKD.
Family and domain databases
HAMAP	MF_00563. [Tree]
InterPro	IPR015878. Ado_hCys_hydrolase_NAD-bd. IPR000043. S-Ado-L-homoCys_hydrolase. IPR020082. S-Ado-L-homoCys_hydrolase_CS. [Graphical view]
PANTHER	PTHR23420. Ad_hcy_hydrolase. 1 hit.
Pfam	PF05221. AdoHcyase. 1 hit. PF00670. AdoHcyase_NAD. 1 hit. [Graphical view]
PIRSF	PIRSF001109. Ad_hcy_hydrolase. 1 hit.
TIGRFAMs	TIGR00936. ahcY. 1 hit.
PROSITE	PS00738. ADOHCYASE_1. 1 hit. PS00739. ADOHCYASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAHH_BRASB

Accession

Primary (citable) accession number: A5ENA7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 12, 2007
Last modified:	November 3, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)