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A5EMF5 (DDL_BRASB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:BBta_5374
OrganismBradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182) [Complete proteome] [HAMAP]
Taxonomic identifier288000 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium

Protein attributes

Sequence length367 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 367367D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341064

Regions

Domain145 – 351207ATP-grasp
Nucleotide binding174 – 22956ATP By similarity

Sites

Metal binding3061Magnesium or manganese 1 By similarity
Metal binding3181Magnesium or manganese 1 By similarity
Metal binding3181Magnesium or manganese 2 By similarity
Metal binding3201Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A5EMF5 [UniParc].

Last modified June 12, 2007. Version 1.
Checksum: B41FF9648B202E9F

FASTA36738,938
        10         20         30         40         50         60 
MAHNTTRVAI LFGGRSAEHD VSRASAANIF RSLDASRYAV TLIGITPEGR WVLADAVNAR 

        70         80         90        100        110        120 
TAQALTVPPD GPQIVLLPAG QGRALVLGDG AAAPRELTFD VIFPVLHGPN GEDGTMQGAL 

       130        140        150        160        170        180 
ELADVAYVGA RVMGSAAAMD KDVAKRLLRD AGLPIVPFVT MTAAAPVSYE DAVHAVGCSE 

       190        200        210        220        230        240 
LFIKPANLGS SVGISKARTP QEFAAACDLA LRFDGKILIE RCISPVREIE CAVLEHADGE 

       250        260        270        280        290        300 
VKASELGEIV PASSHGFYSY AAKYTDASGA SLHVPAQVEP GLAQRIRKMA TEVFGVLCCE 

       310        320        330        340        350        360 
SLARVDFFVH GEEVYVNEVN TLPGFTNISM YPKMWEATGL PQPALMDALI AHALARHARL 


RQLASQR 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000494 Genomic DNA. Translation: ABQ37349.1.
RefSeqYP_001241255.1. NC_009485.1.

3D structure databases

ProteinModelPortalA5EMF5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING288000.BBta_5374.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABQ37349; ABQ37349; BBta_5374.
GeneID5150179.
KEGGbbt:BBta_5374.
PATRIC21211447. VBIBraSp29847_5379.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAQIDVIFP.
OrthoDBEOG64BQ73.

Enzyme and pathway databases

BioCycBSP288000:GJBR-5139-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_BRASB
AccessionPrimary (citable) accession number: A5EMF5
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 12, 2007
Last modified: May 14, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways