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A5EKR5 (A5EKR5_BRASB) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesSubmitted name:
DNA topoisomerase I EMBL ABQ36759.1
EC=5.99.1.2 EMBL ABQ36759.1
Gene names
Name:topA EMBL ABQ36759.1
Ordered Locus Names:BBta_4733
OrganismBradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182) [Complete proteome] [HAMAP] EMBL ABQ36759.1
Taxonomic identifier288000 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length916 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. SAAS SAAS005733

Sequence similarities

Contains 1 Toprim domain. SAAS SAAS023406

Sequences

Sequence LengthMass (Da)Tools
A5EKR5 [UniParc].

Last modified June 12, 2007. Version 1.
Checksum: 607602473A9DF102

FASTA91699,821
        10         20         30         40         50         60 
MNIVIVESPA KAKTINKYLG SSYEVLASFG HVRDLPAKNG SVDPDENFRM IWEVDPKAAG 

        70         80         90        100        110        120 
RLNDIAKSLK NADRLILATD PDREGEAISW HVLEVLKEKR ALKDQKIERV VFNAITKQAV 

       130        140        150        160        170        180 
SEAMKHPRQI DGALVDAYMA RRALDYLVGF TLSPVLWRKL PGARSAGRVQ SVALRLVCDR 

       190        200        210        220        230        240 
ELEIEKFVPR EYWSLIATLA TPRGDTFEAR LVGADGKKIQ RLDIGTGAEA EDFKQALNAA 

       250        260        270        280        290        300 
SYTVATVDAK PARRNPQAPF TTSTLQQEAS RKLGFAPAHT MRIAQRLYEG IDIGGETTGL 

       310        320        330        340        350        360 
ITYMRTDGVQ IDGSAITQAR KVIGEIYGNK YVPDSPRQYQ TKAKNAQEAH EAIRPTDLSR 

       370        380        390        400        410        420 
KPADLRKRLD DDQAKLYELI WTRTIASQME SAELERTTVD IIAKAGGRTL ELRATGQVVK 

       430        440        450        460        470        480 
FDGFLALYQE GRDDEEDEDS RRLPQMSPNE PLKRQNLAVT QHFTEPPPRF SEASLVKRME 

       490        500        510        520        530        540 
ELGIGRPSTY ASILDVLKAR GYVKLEKKRL HGEDKGRVVI AFLENFFRRY VEYDFTADLE 

       550        560        570        580        590        600 
EQLDRISNNE IAWQQVLKDF WTGFIGAVDD IKDLRVAQVL DALDEMLGPH IYPPRADGGD 

       610        620        630        640        650        660 
VRQCPTCGTG RLNLKAGKFG AFVGCSNYPE CRYTRPLAAD SEAAADRILG QDPDSGLDVA 

       670        680        690        700        710        720 
VKAGRFGPYI QLGEQKDYAE GEKPKRAGIP KGTQPSDVDL DLALKLLSLP REIGKHPETG 

       730        740        750        760        770        780 
LPITAGLGRF GPFVKHDKTY ASLEAGDEVF DIGLNRAVTL IAEKVAKGPS KRFGADPGKA 

       790        800        810        820        830        840 
LGDHPSLGPV AVKAGRYGAY VTAGGVNATI PGDKEKDTIT LAEAIALLDE RAAKGGGKAK 

       850        860        870        880        890        900 
GAKKAAKPAK AAAKPKAGGD DDSPKPAKKA VAKKAATKPK SESTSKARAP VAKAAKTSAK 

       910 
PATKATPKKS AGKARG

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000494 Genomic DNA. Translation: ABQ36759.1.
RefSeqYP_001240665.1. NC_009485.1.

3D structure databases

ProteinModelPortalA5EKR5.
ModBaseSearch...

Protein-protein interaction databases

STRINGA5EKR5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5153124.
GenomeReviewsGene locus BBta_4733 in contig CP000494_GR.
KEGGbbt:BBta_4733.
PATRIC21210219. VBIBraSp29847_4772.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0550.
HOGENOMHBG512935.
OMAKKVMPRL.
ProtClustDBPRK07561.

Family and domain databases

InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR006171. Toprim_domain.
[Graphical view]
Gene3DG3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits.
G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KOK03168.
PANTHERPTHR11390. Topo_IA. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 1 hit.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. Topo_IA_core. 1 hit.
TIGRFAMsTIGR01051. TopA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameA5EKR5_BRASB
AccessionPrimary (citable) accession number: A5EKR5
Entry history
Integrated into UniProtKB/TrEMBL: June 12, 2007
Last sequence update: June 12, 2007
Last modified: January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry info