Reviewed, UniProtKB/Swiss-Prot A5EKQ8 (PYRB_BRASB)

Names and origin

Protein names

Recommended name:
    Aspartate carbamoyltransferase
    EC=2.1.3.2
Alternative name(s):
    Aspartate transcarbamylase
      Short name=ATCase

Gene names

Name:	pyrB
Ordered Locus Names:	BBta_4725

Organism

Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182) [Complete proteome] [HAMAP]

Taxonomic identifier

288000 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 2/6.
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro amino acid metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro aspartate carbamoyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 317	317	Aspartate carbamoyltransferase		PRO_0000301557

Sequences

	Sequence		Length	Mass (Da)
	A5EKQ8-1 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 448395D1D4B601C7	FASTA	317	34,506
	10 20 30 40 50 60 MATSPKSTFV LGHRHLLGIE GLSAHDITGL LDLSEEYVEL NRQVDKKRTV LRGRTQVNLF 70 80 90 100 110 120 FEASTRTQSS FELAGKRLGA DVMNMSVSSS SIKKGETLMD TAVTLNAMHP DILVVRHHAS 130 140 150 160 170 180 GAVELLARKV DGSVINAGDG AHEHPTQALL DALTIRRNKG RLEGLVIAIC GDVLHSRVAR 190 200 210 220 230 240 SNILLLNTMG ARVRVVAPST LLPPGIERMG VEVARDMREG LNGADIVMML RLQRERMNGS 250 260 270 280 290 300 FVPSSSEYFQ YFGLDQKKLA YAKPDALVMH PGPMNRGVEI DSIVADGAQS LIREQVEMGV 310 AVRMAVLEAL ARNLPNA

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000494 Genomic DNA. Translation: ABQ36752.1.
RefSeq	YP_001240658.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5151413.
GenomeReviews	Gene locus BBta_4725 in contig CP000494_GR.
KEGG	bbt:BBta_4725.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00001. [Tree]
InterPro	IPR006130. Asp/Orn_carbamoyltranf. IPR006132. Asp/Orn_carbamoyltranf_P_bd. IPR006131. Asp_carbamoyltransf_Asp/Orn_bd. IPR002082. Aspartate_carbamoyltransf_euk. [Graphical view]
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00101. ATCASE.
TIGRFAMs	TIGR00670. asp_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRB_BRASB

Accession

Primary (citable) accession number: A5EKQ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	June 12, 2007
Last modified:	November 25, 2008
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)