A5EKL9 (ACPS_BRASB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Holo-[acyl-carrier-protein] synthase
Short name=Holo-ACP synthase
EC=2.7.8.7

Alternative name(s):
4'-phosphopantetheinyl transferase AcpS

Gene names

Name:	acpS
Ordered Locus Names:	BBta_4685

Organism

Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182) [Complete proteome] [HAMAP]

Taxonomic identifier

288000 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	139 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transfers the 4'-phosphopantetheine moiety from coenzyme A to a Ser of acyl-carrier-protein By similarity. HAMAP-Rule MF_00101
Catalytic activity	CoA-(4'-phosphopantetheine) + apo-[acyl-carrier-protein] = adenosine 3',5'-bisphosphate + holo-[acyl-carrier-protein]. HAMAP-Rule MF_00101
Cofactor	Magnesium By similarity. HAMAP-Rule MF_00101
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00101.
Sequence similarities	Belongs to the P-Pant transferase superfamily. AcpS family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Fatty acid metabolism Lipid biosynthesis Lipid metabolism
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	fatty acid biosynthetic process Inferred from electronic annotation. Source: HAMAP macromolecule biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	holo-[acyl-carrier-protein] synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 139	139	Holo-[acyl-carrier-protein] synthase HAMAP-Rule MF_00101		PRO_1000008391
Sites
	Metal binding	8	1	Magnesium By similarity
	Metal binding	61	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5EKL9 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 15A5C307F813F237	FASTA	139	15,212
	10 20 30 40 50 60 MIIGIGSDLI DIRRVAEVIE RHGDRFLNRI FTEAERAKAE RRAKNEKMVV ATYAKRFAAK 70 80 90 100 110 120 EACSKALGTG IRHGVWWRDM GVVNLPGGRP TMQLTGGAAE RLKALTPAGH DARIDLSITD 130 DWPLAQAFVI ISAVSLATS

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BTAi1 / ATCC BAA-1182.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000494 Genomic DNA. Translation: ABQ36713.1.
RefSeq	YP_001240619.1. NC_009485.1.
3D structure databases
ProteinModelPortal	A5EKL9.
ModBase	Search...
Protein-protein interaction databases
STRING	288000.BBta_4685.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABQ36713; ABQ36713; BBta_4685.
GeneID	5151985.
KEGG	bbt:BBta_4685.
PATRIC	21210127. VBIBraSp29847_4727.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0736.
HOGENOM	HOG000014316.
KO	K00997.
OMA	FWKDMGV.
ProtClustDB	PRK00070.
Enzyme and pathway databases
BioCyc	BSP288000:GJBR-4481-MONOMER.
Family and domain databases
Gene3D	3.90.470.20. 1 hit.
HAMAP	MF_00101. AcpS.
InterPro	IPR008278. 4-PPantetheinyl_Trfase_SF. IPR002582. ACPS. IPR004568. PPantethiene-prot_Trfase_dom. [Graphical view]
Pfam	PF01648. ACPS. 1 hit. [Graphical view]
ProDom	PD004282. PPantethiene-prot_Trfase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF56214. 4-PPT_transf. 1 hit.
TIGRFAMs	TIGR00516. acpS. 1 hit. TIGR00556. pantethn_trn. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACPS_BRASB

Accession

Primary (citable) accession number: A5EKL9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 12, 2007
Last modified:	May 1, 2013
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program