Reviewed, UniProtKB/Swiss-Prot A5EK49 (DXR_BRASB)

Names and origin

Protein names

Recommended name:
    1-deoxy-D-xylulose 5-phosphate reductoisomerase
      Short name=DXP reductoisomerase
    EC=1.1.1.267
Alternative name(s):
    1-deoxyxylulose-5-phosphate reductoisomerase
    2-C-methyl-D-erythritol 4-phosphate synthase

Gene names

Name:	dxr
Ordered Locus Names:	BBta_4511

Organism

Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182) [Complete proteome] [HAMAP]

Taxonomic identifier

288000 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity.
Catalytic activity	2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH. HAMAP MF_00183
Cofactor	Divalent cation By similarity.
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 1/6. HAMAP MF_00183
Sequence similarities	Belongs to the DXR family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1-deoxy-D-xylulose-5-phosphate reductoisomerase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 407	407	1-deoxy-D-xylulose 5-phosphate reductoisomerase HAMAP MF_00183		PRO_1000020221
Regions
	Nucleotide binding	22 – 51	30	NADP By similarity
Sites
	Metal binding	162	1	Divalent metal cation By similarity
	Metal binding	164	1	Divalent metal cation By similarity
	Metal binding	233	1	Divalent metal cation By similarity
	Binding site	137	1	Substrate By similarity
	Binding site	164	1	Substrate By similarity
	Binding site	188	1	Substrate By similarity
	Binding site	211	1	Substrate By similarity
	Binding site	233	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5EK49-1 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 8CE3F275055AE2A9	FASTA	407	42,610
	10 20 30 40 50 60 MSAVPLRNNK PGRAEVRSIT VLGATGSIGE STMDLLRGAP ERYRVEALTG NSNVQGLAKL 70 80 90 100 110 120 AREFKPGFVA VADPARLNEL RDALAGTGIA CGAGESAIIE AAARPADWVM AAVSGAAGLK 130 140 150 160 170 180 PALAAVDRGA TVALANKECL VCAGDFFMQR AAQAGACILP ADSEHNALFQ ALSSGNREEL 190 200 210 220 230 240 VRVIITASGG PFRTWAAADI EQATLAQALK HPNWSMGQKI TIDSASMMNK GLEVIEASYL 250 260 270 280 290 300 FALSPDEIDV LVHPQSIVHG MVEFSDRSVV AQLGAPDMRI PIAHCLGWPD RIAGPSARLD 310 320 330 340 350 360 LAKIGTLTFE APDFVRFPGL RLAYDSLRTG RGATTVYNAA NEVAVAAFAA GQIRFGAIAR 370 380 390 400 LVEATLEHWT RSGNQAPLTS ADDAIAIDHD ARKTAAGLLP QIAAKAS

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000494 Genomic DNA. Translation: ABQ36543.1.
RefSeq	YP_001240449.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A5EK49.
Genome annotation databases
GeneID	5154549.
GenomeReviews	Gene locus BBta_4511 in contig CP000494_GR.
KEGG	bbt:BBta_4511.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	IHSMVEY.
Family and domain databases
HAMAP	MF_00183. [Tree]
InterPro	IPR003821. DXP_reductoisomerase. IPR013644. DXP_reductoisomerase_C. IPR013512. DXP_reductoisomerase_N. [Graphical view]
Pfam	PF08436. DXP_redisom_C. 1 hit. PF02670. DXP_reductoisom. 1 hit. [Graphical view]
PIRSF	PIRSF006205. Dxp_reductismrs. 1 hit.
TIGRFAMs	TIGR00243. Dxr. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DXR_BRASB

Accession

Primary (citable) accession number: A5EK49

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 12, 2007
Last modified:	November 3, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)