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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei243ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:BBta_4501
OrganismiBradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182)
Taxonomic identifieri288000 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
Proteomesi
  • UP000000246 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000018761 – 474Glutamate--tRNA ligaseAdd BLAST474

Proteomic databases

PRIDEiA5EK39

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi288000.BBta_4501

Structurei

3D structure databases

ProteinModelPortaliA5EK39
SMRiA5EK39
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi11 – 21"HIGH" regionUniRule annotationAdd BLAST11
Motifi240 – 244"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252722
KOiK01885
OMAiAYYAFDT
OrthoDBiPOG091H021W

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A5EK39-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSPIVTRFA PSPTGFLHIG GARTALFNWL YARGRCGKML LRIEDTDRER
60 70 80 90 100
STTAAIDAIL DGLKWLELDW DGEVIYQYSR AARHREVAEQ LLASGMAYRC
110 120 130 140 150
YATAEELAAM REKARAEGRT RLYDGMWRDR DPKDAPGDVK PTIRLRAPLT
160 170 180 190 200
GETVIEDQVQ GRVVWQNENL DDLVLLRGDG NPTYMLAVVV DDHDMGVTHV
210 220 230 240 250
IRGDDHLINA ARQKQIYDAL GWTVPSMSHI PLIHGPDGSK LSKRHGALGV
260 270 280 290 300
DAYRAMGYLP SALRNYLVRL GWSHGDQEIF STEEMIKAFD LPAIGRSAAR
310 320 330 340 350
FDFAKLENLN GHYIRHSDDA ALVRQFEDVL NYVPNGATLK AKLNDATRAQ
360 370 380 390 400
LTQAMPSLKE RAKTLLELID GAAFLFADRP LPIDAKAAAL LTPDSRALIG
410 420 430 440 450
RLHDALAAVE PWSGPATEAA LRAFAETNNL KLGAVAQPLR AALTGRTTSP
460 470
GIFDVLAILG RDESLGRLKD QTTS
Length:474
Mass (Da):52,284
Last modified:June 12, 2007 - v1
Checksum:iB83AD3D87BC1F459
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000494 Genomic DNA Translation: ABQ36533.1
RefSeqiWP_012044529.1, NC_009485.1

Genome annotation databases

EnsemblBacteriaiABQ36533; ABQ36533; BBta_4501
KEGGibbt:BBta_4501

Similar proteinsi

Entry informationi

Entry nameiSYE_BRASB
AccessioniPrimary (citable) accession number: A5EK39
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 12, 2007
Last modified: May 23, 2018
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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