Reviewed, UniProtKB/Swiss-Prot A5EK16 (KDSA_BRASB)

Names and origin

Protein names

Recommended name:
    2-dehydro-3-deoxyphosphooctonate aldolase
    EC=2.5.1.55
Alternative name(s):
    Phospho-2-dehydro-3-deoxyoctonate aldolase
    3-deoxy-D-manno-octulosonic acid 8-phosphate synthetase
    KDO-8-phosphate synthetase
      Short name=KDO 8-P synthase
      Short name=KDOPS

Gene names

Name:	kdsA
Ordered Locus Names:	BBta_4474

Organism

Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182) [Complete proteome] [HAMAP]

Taxonomic identifier

288000 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + D-arabinose 5-phosphate + H2O = 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate. HAMAP MF_00056
Pathway	Carbohydrate biosynthesis; 3-deoxy-D-manno-octulosonate biosynthesis; 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate: step 2/3. HAMAP MF_00056 Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. HAMAP MF_00056
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the kdsA family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	keto-3-deoxy-D-manno-octulosonic acid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-deoxy-8-phosphooctulonate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	2-dehydro-3-deoxyphosphooctonate aldolase HAMAP MF_00056		PRO_0000304433

Sequences

	Sequence		Length	Mass (Da)
	A5EK16-1 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: D1A597E1D718C7F9	FASTA	286	29,663
	10 20 30 40 50 60 MTTAATAAAI VTAGSVRFGN DLPLAVIAGP CQLESRGHAL EVASALKEIA TRLNIGLVYK 70 80 90 100 110 120 TSFDKANRTS GSAARGLGLA QSLPIFAEIR SSLGLPVLTD IHDASQCAEV AQAVDVLQIP 130 140 150 160 170 180 AFLCRQTDLL LAAAATGKVV NVKKGQFLAP WDMANVVAKI TGAGNPNVLA TERGVSFGYN 190 200 210 220 230 240 TLISDMRSLP IMARTTGAPV IFDATHSVQQ PGGQGTSSGG QREFVPVLAR AAVAVGVAGV 250 260 270 280 FIETHPDPDH APSDGPNMVP LAEFEGLLRR LMAFDKLAKA PATDTR

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000494 Genomic DNA. Translation: ABQ36510.1.
RefSeq	YP_001240416.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A5EK16.
Genome annotation databases
GeneID	5155571.
GenomeReviews	Gene locus BBta_4474 in contig CP000494_GR.
KEGG	bbt:BBta_4474.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VYKTSFD.
Family and domain databases
HAMAP	MF_00056. [Tree]
InterPro	IPR006218. DAHP1/KDSA. IPR006269. KDO8P_synthase. [Graphical view]
PANTHER	PTHR21057:SF2. KDO8P_synthase. 1 hit.
Pfam	PF00793. DAHP_synth_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01362. KDO8P_synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDSA_BRASB

Accession

Primary (citable) accession number: A5EK16

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	June 12, 2007
Last modified:	November 3, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)