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Reviewed, UniProtKB/Swiss-Prot A5EK14 (QUEF_BRASB)

Last modified February 9, 2010. Version 26. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    NADPH-dependent 7-cyano-7-deazaguanine reductase
    EC=1.7.1.13
Alternative name(s):
    7-cyano-7-carbaguanine reductase
    PreQ(0) reductase
    NADPH-dependent nitrile oxidoreductase
Gene names
Name: queF
Ordered Locus Names: BBta_4472
OrganismBradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182) [Complete proteome] [HAMAP]
Taxonomic identifier288000 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium

Protein attributes

Sequence length158 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

General annotation (Comments)

Function

Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP MF_00818

Catalytic activity

7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00818

Pathway

tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00818

Subcellular location

Cytoplasm Probable HAMAP MF_00818.

Sequence similarities

Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 158158NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00818
PRO_1000062377

Sequences

Sequence LengthMass (Da)Tools
A5EK14-1 [UniParc].

Last modified June 12, 2007. Version 1.
Checksum: 824DEA2EF6555DE6

FASTA15817,675
        10         20         30         40         50         60 
MAKRSIKSET SPELQLGRPV TAPDSPETAR LDRVPNPQKD TDYLARFTVP EFTSLCPVTG 

        70         80         90        100        110        120 
QPDFAHLVID YAPGPWLVES KSLKLYLASF RNHGAFHEDC TVAIGKRLAT ELKPKWLRIG 

       130        140        150 
GYWYPRGGIP IDVFWQTGKL PKGLWVPDQG VAPYRGRG 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000494 Genomic DNA. Translation: ABQ36508.1.
RefSeqYP_001240414.1.

3D structure databases

SMRA5EK14. Positions 58-139.
ModBaseSearch...

Protein-protein interaction databases

STRINGA5EK14.

Genome annotation databases

GeneID5155577.
GenomeReviewsGene locus BBta_4472 in contig CP000494_GR.
KEGGbbt:BBta_4472.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0780.
HOGENOMHBG294103.
OMALYLASFR.

Family and domain databases

HAMAPMF_00818. QueF_type1.
[Tree]
InterProIPR016856. CN_OxRdtase_NADPH-dep_QueF.
IPR020602. GTP_CycHdrlase_I/CN_OxRdtase.
[Graphical view]
PfamPF01227. GTP_cyclohydroI. 1 hit.
[Graphical view]
PIRSFPIRSF027377. Nitrile_oxidored_QueF. 1 hit.
TIGRFAMsTIGR03139. QueF-II. 1 hit.
ProtoNetSearch...

Entry information

Entry nameQUEF_BRASB
AccessionPrimary (citable) accession number: A5EK14
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: June 12, 2007
Last modified: February 9, 2010
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents