Reviewed, UniProtKB/Swiss-Prot A5EIE6 (SYD_BRASB)

Names and origin

Protein names

Recommended name:
    Aspartyl-tRNA synthetase
    EC=6.1.1.12
Alternative name(s):
    Aspartate--tRNA ligase
      Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	BBta_3864

Organism

Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182) [Complete proteome] [HAMAP]

Taxonomic identifier

288000 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	590 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 590	590	Aspartyl-tRNA synthetase HAMAP MF_00044		PRO_1000006641

Sequences

	Sequence		Length	Mass (Da)
	A5EIE6-1 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 512DE030B649AF4D	FASTA	590	66,606
	10 20 30 40 50 60 MHRYRTHTCG ALRDSNIGET VRLSGWCHRI RDHGGVLFVD LRDHYGITQC VVDPDSKAFG 70 80 90 100 110 120 LAEKLRSEWV VRMEGKVRRR PEGTDNAELP TGQVELYVAD IEVLGPAAEL PLPVFGEQEY 130 140 150 160 170 180 PEDIRLKYRF LDLRREKLHQ NIMTRGAIID SMRRRMKEQG FFEFQTPILT ASSPEGARDF 190 200 210 220 230 240 LVPSRIHPGK FYALPQAPQQ YKQLLMMSGF DRYFQIAPCF RDEDPRADRL PGEFYQLDVE 250 260 270 280 290 300 MSFVEQEDVF AAMEPVITGV FEDFAKGKPV TKGWPRIPFA EALRKYGTDK PDLRNPIEMQ 310 320 330 340 350 360 DVSEHFRGSG FKVFARMLED TKNQVWAIPA PGGGSRAFCD RMNSWAQGEG QPGLGYIMWR 370 380 390 400 410 420 EGGEGAGPLA NNIGPERTAA IRTQLGTKEG DAAFFVAGDP EKFWKFAGLA RTKVGEELNL 430 440 450 460 470 480 IDKDRFALAW IVDFPMYEYN EDDKKVDFSH NPFSMPQGGL EALQTKDPLT IKAFQYDIAC 490 500 510 520 530 540 NGYEIASGGI RNHKPEAMVK AFEIAGYGEQ EVVDRFGGMY RAFQYGAPPH GGMAAGVDRI 550 560 570 580 590 VMLLCGTTNL REISLFPMNQ QAMDLLMGAP SEATTKQLRE LHIRTNLPNK

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed: 17540897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000494 Genomic DNA. Translation: ABQ35940.1.
RefSeq	YP_001239846.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5150013.
GenomeReviews	Gene locus BBta_3864 in contig CP000494_GR.
KEGG	bbt:BBta_3864.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A5EIE6. YQLDVEM.
Family and domain databases
HAMAP	MF_00044. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR002312. Asp-tRNA-synth_IIb. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR018153. Asp-tRNA-synth_IIb_C_bac/mt. IPR004115. GAD. IPR012340. NA-bd_OB-fold. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00459. aspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_BRASB

Accession

Primary (citable) accession number: A5EIE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 12, 2007
Last modified:	June 16, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)