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A5EIE6 (SYD_BRASB) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Aspartate--tRNA ligase
EC=6.1.1.12
Alternative name(s):
Aspartyl-tRNA synthetase
Short name=AspRS
Gene names
Name:aspS
Ordered Locus Names:BBta_3864
OrganismBradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182) [Complete proteome] [HAMAP]
Taxonomic identifier288000 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length590 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044_B

Subunit structure

Homodimer By similarity. HAMAP MF_00044_B

Subcellular location

Cytoplasm By similarity HAMAP MF_00044_B.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processtRNA aminoacylation for protein translation

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

aspartate-tRNA ligase activity

Inferred from electronic annotation. Source: EC

nucleic acid binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 590590Aspartate--tRNA ligase HAMAP MF_00044_B
PRO_1000006641

Sequences

Sequence LengthMass (Da)Tools
A5EIE6 [UniParc].

Last modified June 12, 2007. Version 1.
Checksum: 512DE030B649AF4D

FASTA59066,606
        10         20         30         40         50         60 
MHRYRTHTCG ALRDSNIGET VRLSGWCHRI RDHGGVLFVD LRDHYGITQC VVDPDSKAFG 

        70         80         90        100        110        120 
LAEKLRSEWV VRMEGKVRRR PEGTDNAELP TGQVELYVAD IEVLGPAAEL PLPVFGEQEY 

       130        140        150        160        170        180 
PEDIRLKYRF LDLRREKLHQ NIMTRGAIID SMRRRMKEQG FFEFQTPILT ASSPEGARDF 

       190        200        210        220        230        240 
LVPSRIHPGK FYALPQAPQQ YKQLLMMSGF DRYFQIAPCF RDEDPRADRL PGEFYQLDVE 

       250        260        270        280        290        300 
MSFVEQEDVF AAMEPVITGV FEDFAKGKPV TKGWPRIPFA EALRKYGTDK PDLRNPIEMQ 

       310        320        330        340        350        360 
DVSEHFRGSG FKVFARMLED TKNQVWAIPA PGGGSRAFCD RMNSWAQGEG QPGLGYIMWR 

       370        380        390        400        410        420 
EGGEGAGPLA NNIGPERTAA IRTQLGTKEG DAAFFVAGDP EKFWKFAGLA RTKVGEELNL 

       430        440        450        460        470        480 
IDKDRFALAW IVDFPMYEYN EDDKKVDFSH NPFSMPQGGL EALQTKDPLT IKAFQYDIAC 

       490        500        510        520        530        540 
NGYEIASGGI RNHKPEAMVK AFEIAGYGEQ EVVDRFGGMY RAFQYGAPPH GGMAAGVDRI 

       550        560        570        580        590 
VMLLCGTTNL REISLFPMNQ QAMDLLMGAP SEATTKQLRE LHIRTNLPNK

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000494 Genomic DNA. Translation: ABQ35940.1.
RefSeqYP_001239846.1. NC_009485.1.

3D structure databases

ProteinModelPortalA5EIE6.
ModBaseSearch...

Protein-protein interaction databases

STRINGA5EIE6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5150013.
GenomeReviewsGene locus BBta_3864 in contig CP000494_GR.
KEGGbbt:BBta_3864.
PATRIC21208521. VBIBraSp29847_3935.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0173.
HOGENOMHBG396032.
OMAYQLDVEM.
ProtClustDBPRK00476.

Family and domain databases

HAMAPMF_00044_B. Asp_tRNA_synth_B.
[Tree]
InterProIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004524. Asp-tRNA-synth_IIb_bac/mt.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR004115. GAD_dom.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR004365. NA-bd_OB_tRNA-helicase.
[Graphical view]
Gene3DG3DSA:3.30.1360.30. GAD_dom. 1 hit.
G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KOK01876.
PANTHERPTHR22594. aa-tRNA-synt_II. 1 hit.
PTHR22594:SF5. AspS_bac. 1 hit.
PfamPF02938. GAD. 1 hit.
PF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti. 1 hit.
[Graphical view]
PRINTSPR01042. TRNASYNTHASP.
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
SSF55261. SSF55261. 1 hit.
TIGRFAMsTIGR00459. AspS_bact. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYD_BRASB
AccessionPrimary (citable) accession number: A5EIE6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 12, 2007
Last modified: January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

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