A5EIB4 (AMPA_BRASB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable cytosol aminopeptidase
EC=3.4.11.1

Alternative name(s):
Leucine aminopeptidase
Short name=LAP
EC=3.4.11.10
Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	BBta_3832

Organism

Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182) [Complete proteome] [HAMAP]

Taxonomic identifier

288000 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP-Rule MF_00181
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP-Rule MF_00181 Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
Cofactor	Binds 2 manganese ions per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aminopeptidase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP metalloexopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 499	499	Probable cytosol aminopeptidase HAMAP-Rule MF_00181		PRO_1000019887
Sites
	Active site	275	1	Potential
	Active site	349	1	Potential
	Metal binding	263	1	Manganese 2 By similarity
	Metal binding	268	1	Manganese 1 By similarity
	Metal binding	268	1	Manganese 2 By similarity
	Metal binding	286	1	Manganese 2 By similarity
	Metal binding	345	1	Manganese 1 By similarity
	Metal binding	347	1	Manganese 1 By similarity
	Metal binding	347	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	A5EIB4 [UniParc]. Last modified June 12, 2007. Version 1. Checksum: 6CE6EBAE4B899EF5	FASTA	499	52,627
	10 20 30 40 50 60 MSDTVKVGFV PLSTAPRGIL VAFCDESLRL GAATDKALGG AAELIQRAAA AARFKGKDGA 70 80 90 100 110 120 VLDLLAPQGI KIQRLLVIGT GKSGDHKEKD FLKYGGTLAG KLNAGTEPVM VMAELPSGPM 130 140 150 160 170 180 TPDQASSLAA GIRLRAYRFD RYKTKKKDEE AAVSAAISLA VADVAAAKKA FAPRSHVTDG 190 200 210 220 230 240 VILARDLVNE PPNVLYPEEF AKRASALRKL GVTVEILDVP AMKKLGMGAL LGVGQGSARP 250 260 270 280 290 300 SRTVVMRYNG GKKGDQPVAF IGKGVCFDTG GISIKPAGSM EDMKGDMGGA ACVVGLMHAL 310 320 330 340 350 360 AARKAKANVI GAIGLVENMP DGNAQRPGDI VTSMSGQTIE IINTDAEGRL VLADVLWYVA 370 380 390 400 410 420 TKFKPKFMID LATLTGAIMV ALGTEHAGLF ANNDDLAEKL LKAGQETGEK VWRMPLGPEY 430 440 450 460 470 480 DKLIDSQFAD MKNTGGRHGG SITAAQFLQR FVSDTPWAHL DIAGTAMGAP KSEVNQSWGS 490 GYGVRLLDRL VWDCYEARS

References

[1]

"Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia." Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C., Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L., Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z. , Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A., Medigue C., Sadowsky M. Science 316:1307-1312(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BTAi1 / ATCC BAA-1182.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000494 Genomic DNA. Translation: ABQ35908.1.
RefSeq	YP_001239814.1. NC_009485.1.
3D structure databases
ProteinModelPortal	A5EIB4.
ModBase	Search...
Protein-protein interaction databases
STRING	288000.BBta_3832.
Protein family/group databases
MEROPS	M17.003.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABQ35908; ABQ35908; BBta_3832.
GeneID	5154721.
KEGG	bbt:BBta_3832.
PATRIC	21208457. VBIBraSp29847_3903.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0260.
HOGENOM	HOG000243129.
KO	K01255.
OMA	TPVDFAN.
ProtClustDB	PRK00913.
Enzyme and pathway databases
BioCyc	BSP288000:GJBR-3656-MONOMER.
Family and domain databases
HAMAP	MF_00181. Cytosol_peptidase_M17.
InterPro	IPR011356. Leucine_aapep/pepB. IPR000819. Peptidase_M17_C. IPR023042. Peptidase_M17_leu_NH2_pept. IPR008283. Peptidase_M17_N. [Graphical view]
PANTHER	PTHR11963:SF3. PTHR11963:SF3. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_BRASB

Accession

Primary (citable) accession number: A5EIB4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 12, 2007
Last modified:	May 1, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program