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A5EIB4 (AMPA_BRASB) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable cytosol aminopeptidase

EC=3.4.11.1
Alternative name(s):
Leucine aminopeptidase
Short name=LAP
EC=3.4.11.10
Leucyl aminopeptidase
Gene names
Name:pepA
Ordered Locus Names:BBta_3832
OrganismBradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182) [Complete proteome] [HAMAP]
Taxonomic identifier288000 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium

Protein attributes

Sequence length499 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP-Rule MF_00181

Catalytic activity

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP-Rule MF_00181

Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.

Cofactor

Binds 2 manganese ions per subunit By similarity. HAMAP-Rule MF_00181

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00181.

Sequence similarities

Belongs to the peptidase M17 family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandManganese
Metal-binding
   Molecular functionAminopeptidase
Hydrolase
Protease
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processproteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionaminopeptidase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

manganese ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metalloexopeptidase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 499499Probable cytosol aminopeptidase HAMAP-Rule MF_00181
PRO_1000019887

Sites

Active site2751 Potential
Active site3491 Potential
Metal binding2631Manganese 2 By similarity
Metal binding2681Manganese 1 By similarity
Metal binding2681Manganese 2 By similarity
Metal binding2861Manganese 2 By similarity
Metal binding3451Manganese 1 By similarity
Metal binding3471Manganese 1 By similarity
Metal binding3471Manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A5EIB4 [UniParc].

Last modified June 12, 2007. Version 1.
Checksum: 6CE6EBAE4B899EF5

FASTA49952,627
        10         20         30         40         50         60 
MSDTVKVGFV PLSTAPRGIL VAFCDESLRL GAATDKALGG AAELIQRAAA AARFKGKDGA 

        70         80         90        100        110        120 
VLDLLAPQGI KIQRLLVIGT GKSGDHKEKD FLKYGGTLAG KLNAGTEPVM VMAELPSGPM 

       130        140        150        160        170        180 
TPDQASSLAA GIRLRAYRFD RYKTKKKDEE AAVSAAISLA VADVAAAKKA FAPRSHVTDG 

       190        200        210        220        230        240 
VILARDLVNE PPNVLYPEEF AKRASALRKL GVTVEILDVP AMKKLGMGAL LGVGQGSARP 

       250        260        270        280        290        300 
SRTVVMRYNG GKKGDQPVAF IGKGVCFDTG GISIKPAGSM EDMKGDMGGA ACVVGLMHAL 

       310        320        330        340        350        360 
AARKAKANVI GAIGLVENMP DGNAQRPGDI VTSMSGQTIE IINTDAEGRL VLADVLWYVA 

       370        380        390        400        410        420 
TKFKPKFMID LATLTGAIMV ALGTEHAGLF ANNDDLAEKL LKAGQETGEK VWRMPLGPEY 

       430        440        450        460        470        480 
DKLIDSQFAD MKNTGGRHGG SITAAQFLQR FVSDTPWAHL DIAGTAMGAP KSEVNQSWGS 

       490 
GYGVRLLDRL VWDCYEARS 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000494 Genomic DNA. Translation: ABQ35908.1.
RefSeqYP_001239814.1. NC_009485.1.

3D structure databases

ProteinModelPortalA5EIB4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING288000.BBta_3832.

Protein family/group databases

MEROPSM17.003.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABQ35908; ABQ35908; BBta_3832.
GeneID5154721.
KEGGbbt:BBta_3832.
PATRIC21208457. VBIBraSp29847_3903.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0260.
HOGENOMHOG000243129.
KOK01255.
OMATENAISK.
OrthoDBEOG6FV8B3.
ProtClustDBPRK00913.

Enzyme and pathway databases

BioCycBSP288000:GJBR-3656-MONOMER.

Family and domain databases

HAMAPMF_00181. Cytosol_peptidase_M17.
InterProIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PANTHERPTHR11963:SF3. PTHR11963:SF3. 1 hit.
PfamPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSPR00481. LAMNOPPTDASE.
PROSITEPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAMPA_BRASB
AccessionPrimary (citable) accession number: A5EIB4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 12, 2007
Last modified: February 19, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries