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Protein

Probable cytosol aminopeptidase

Gene

pepA

Organism
Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.UniRule annotation

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.UniRule annotation
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 2 manganese ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi263 – 2631Manganese 2UniRule annotation
Metal bindingi268 – 2681Manganese 1UniRule annotation
Metal bindingi268 – 2681Manganese 2UniRule annotation
Active sitei275 – 2751UniRule annotation
Metal bindingi286 – 2861Manganese 2UniRule annotation
Metal bindingi345 – 3451Manganese 1UniRule annotation
Metal bindingi347 – 3471Manganese 1UniRule annotation
Metal bindingi347 – 3471Manganese 2UniRule annotation
Active sitei349 – 3491UniRule annotation

GO - Molecular functioni

  1. aminopeptidase activity Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. metalloexopeptidase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciBSP288000:GJBR-3656-MONOMER.

Protein family/group databases

MEROPSiM17.013.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cytosol aminopeptidaseUniRule annotation (EC:3.4.11.1UniRule annotation)
Alternative name(s):
Leucine aminopeptidaseUniRule annotation (EC:3.4.11.10UniRule annotation)
Short name:
LAPUniRule annotation
Leucyl aminopeptidaseUniRule annotation
Gene namesi
Name:pepAUniRule annotation
Ordered Locus Names:BBta_3832
OrganismiBradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182)
Taxonomic identifieri288000 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
ProteomesiUP000000246 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 499499Probable cytosol aminopeptidasePRO_1000019887Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi288000.BBta_3832.

Structurei

3D structure databases

ProteinModelPortaliA5EIB4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M17 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0260.
HOGENOMiHOG000243129.
KOiK01255.
OMAiGGMEDMK.
OrthoDBiEOG6FV8B3.

Family and domain databases

HAMAPiMF_00181. Cytosol_peptidase_M17.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A5EIB4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDTVKVGFV PLSTAPRGIL VAFCDESLRL GAATDKALGG AAELIQRAAA
60 70 80 90 100
AARFKGKDGA VLDLLAPQGI KIQRLLVIGT GKSGDHKEKD FLKYGGTLAG
110 120 130 140 150
KLNAGTEPVM VMAELPSGPM TPDQASSLAA GIRLRAYRFD RYKTKKKDEE
160 170 180 190 200
AAVSAAISLA VADVAAAKKA FAPRSHVTDG VILARDLVNE PPNVLYPEEF
210 220 230 240 250
AKRASALRKL GVTVEILDVP AMKKLGMGAL LGVGQGSARP SRTVVMRYNG
260 270 280 290 300
GKKGDQPVAF IGKGVCFDTG GISIKPAGSM EDMKGDMGGA ACVVGLMHAL
310 320 330 340 350
AARKAKANVI GAIGLVENMP DGNAQRPGDI VTSMSGQTIE IINTDAEGRL
360 370 380 390 400
VLADVLWYVA TKFKPKFMID LATLTGAIMV ALGTEHAGLF ANNDDLAEKL
410 420 430 440 450
LKAGQETGEK VWRMPLGPEY DKLIDSQFAD MKNTGGRHGG SITAAQFLQR
460 470 480 490
FVSDTPWAHL DIAGTAMGAP KSEVNQSWGS GYGVRLLDRL VWDCYEARS
Length:499
Mass (Da):52,627
Last modified:June 12, 2007 - v1
Checksum:i6CE6EBAE4B899EF5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000494 Genomic DNA. Translation: ABQ35908.1.
RefSeqiWP_012043913.1. NC_009485.1.
YP_001239814.1. NC_009485.1.

Genome annotation databases

EnsemblBacteriaiABQ35908; ABQ35908; BBta_3832.
KEGGibbt:BBta_3832.
PATRICi21208457. VBIBraSp29847_3903.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000494 Genomic DNA. Translation: ABQ35908.1.
RefSeqiWP_012043913.1. NC_009485.1.
YP_001239814.1. NC_009485.1.

3D structure databases

ProteinModelPortaliA5EIB4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi288000.BBta_3832.

Protein family/group databases

MEROPSiM17.013.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABQ35908; ABQ35908; BBta_3832.
KEGGibbt:BBta_3832.
PATRICi21208457. VBIBraSp29847_3903.

Phylogenomic databases

eggNOGiCOG0260.
HOGENOMiHOG000243129.
KOiK01255.
OMAiGGMEDMK.
OrthoDBiEOG6FV8B3.

Enzyme and pathway databases

BioCyciBSP288000:GJBR-3656-MONOMER.

Family and domain databases

HAMAPiMF_00181. Cytosol_peptidase_M17.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BTAi1 / ATCC BAA-1182.

Entry informationi

Entry nameiAMPA_BRASB
AccessioniPrimary (citable) accession number: A5EIB4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 12, 2007
Last modified: April 1, 2015
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.