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A5EIB4

- AMPA_BRASB

UniProt

A5EIB4 - AMPA_BRASB

Protein

Probable cytosol aminopeptidase

Gene

pepA

Organism
Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 51 (01 Oct 2014)
      Sequence version 1 (12 Jun 2007)
      Previous versions | rss
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    • Comment

    Functioni

    Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.UniRule annotation

    Catalytic activityi

    Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.UniRule annotation
    Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.UniRule annotation

    Cofactori

    Binds 2 manganese ions per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi263 – 2631Manganese 2UniRule annotation
    Metal bindingi268 – 2681Manganese 1UniRule annotation
    Metal bindingi268 – 2681Manganese 2UniRule annotation
    Active sitei275 – 2751UniRule annotation
    Metal bindingi286 – 2861Manganese 2UniRule annotation
    Metal bindingi345 – 3451Manganese 1UniRule annotation
    Metal bindingi347 – 3471Manganese 1UniRule annotation
    Metal bindingi347 – 3471Manganese 2UniRule annotation
    Active sitei349 – 3491UniRule annotation

    GO - Molecular functioni

    1. aminopeptidase activity Source: UniProtKB-HAMAP
    2. manganese ion binding Source: UniProtKB-HAMAP
    3. metalloexopeptidase activity Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminopeptidase, Hydrolase, Protease

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciBSP288000:GJBR-3656-MONOMER.

    Protein family/group databases

    MEROPSiM17.003.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable cytosol aminopeptidaseUniRule annotation (EC:3.4.11.1UniRule annotation)
    Alternative name(s):
    Leucine aminopeptidaseUniRule annotation (EC:3.4.11.10UniRule annotation)
    Short name:
    LAPUniRule annotation
    Leucyl aminopeptidaseUniRule annotation
    Gene namesi
    Name:pepAUniRule annotation
    Ordered Locus Names:BBta_3832
    OrganismiBradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182)
    Taxonomic identifieri288000 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
    ProteomesiUP000000246: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 499499Probable cytosol aminopeptidasePRO_1000019887Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi288000.BBta_3832.

    Structurei

    3D structure databases

    ProteinModelPortaliA5EIB4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M17 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0260.
    HOGENOMiHOG000243129.
    KOiK01255.
    OMAiDSQFADM.
    OrthoDBiEOG6FV8B3.

    Family and domain databases

    HAMAPiMF_00181. Cytosol_peptidase_M17.
    InterProiIPR011356. Leucine_aapep/pepB.
    IPR000819. Peptidase_M17_C.
    IPR023042. Peptidase_M17_leu_NH2_pept.
    IPR008283. Peptidase_M17_N.
    [Graphical view]
    PfamiPF00883. Peptidase_M17. 1 hit.
    PF02789. Peptidase_M17_N. 1 hit.
    [Graphical view]
    PRINTSiPR00481. LAMNOPPTDASE.
    PROSITEiPS00631. CYTOSOL_AP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A5EIB4-1 [UniParc]FASTAAdd to Basket

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    MSDTVKVGFV PLSTAPRGIL VAFCDESLRL GAATDKALGG AAELIQRAAA    50
    AARFKGKDGA VLDLLAPQGI KIQRLLVIGT GKSGDHKEKD FLKYGGTLAG 100
    KLNAGTEPVM VMAELPSGPM TPDQASSLAA GIRLRAYRFD RYKTKKKDEE 150
    AAVSAAISLA VADVAAAKKA FAPRSHVTDG VILARDLVNE PPNVLYPEEF 200
    AKRASALRKL GVTVEILDVP AMKKLGMGAL LGVGQGSARP SRTVVMRYNG 250
    GKKGDQPVAF IGKGVCFDTG GISIKPAGSM EDMKGDMGGA ACVVGLMHAL 300
    AARKAKANVI GAIGLVENMP DGNAQRPGDI VTSMSGQTIE IINTDAEGRL 350
    VLADVLWYVA TKFKPKFMID LATLTGAIMV ALGTEHAGLF ANNDDLAEKL 400
    LKAGQETGEK VWRMPLGPEY DKLIDSQFAD MKNTGGRHGG SITAAQFLQR 450
    FVSDTPWAHL DIAGTAMGAP KSEVNQSWGS GYGVRLLDRL VWDCYEARS 499
    Length:499
    Mass (Da):52,627
    Last modified:June 12, 2007 - v1
    Checksum:i6CE6EBAE4B899EF5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000494 Genomic DNA. Translation: ABQ35908.1.
    RefSeqiWP_012043913.1. NC_009485.1.
    YP_001239814.1. NC_009485.1.

    Genome annotation databases

    EnsemblBacteriaiABQ35908; ABQ35908; BBta_3832.
    GeneIDi5154721.
    KEGGibbt:BBta_3832.
    PATRICi21208457. VBIBraSp29847_3903.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000494 Genomic DNA. Translation: ABQ35908.1 .
    RefSeqi WP_012043913.1. NC_009485.1.
    YP_001239814.1. NC_009485.1.

    3D structure databases

    ProteinModelPortali A5EIB4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 288000.BBta_3832.

    Protein family/group databases

    MEROPSi M17.003.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABQ35908 ; ABQ35908 ; BBta_3832 .
    GeneIDi 5154721.
    KEGGi bbt:BBta_3832.
    PATRICi 21208457. VBIBraSp29847_3903.

    Phylogenomic databases

    eggNOGi COG0260.
    HOGENOMi HOG000243129.
    KOi K01255.
    OMAi DSQFADM.
    OrthoDBi EOG6FV8B3.

    Enzyme and pathway databases

    BioCyci BSP288000:GJBR-3656-MONOMER.

    Family and domain databases

    HAMAPi MF_00181. Cytosol_peptidase_M17.
    InterProi IPR011356. Leucine_aapep/pepB.
    IPR000819. Peptidase_M17_C.
    IPR023042. Peptidase_M17_leu_NH2_pept.
    IPR008283. Peptidase_M17_N.
    [Graphical view ]
    Pfami PF00883. Peptidase_M17. 1 hit.
    PF02789. Peptidase_M17_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00481. LAMNOPPTDASE.
    PROSITEi PS00631. CYTOSOL_AP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: BTAi1 / ATCC BAA-1182.

    Entry informationi

    Entry nameiAMPA_BRASB
    AccessioniPrimary (citable) accession number: A5EIB4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: June 12, 2007
    Last modified: October 1, 2014
    This is version 51 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3